Lidstrom:Pouring Media Plates
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- Put date on plates. Some antibiotics aren't very long lasting.
- For example, Ampicillin plates only last 2-3 weeks. (is there a citation for this? -Janet 2012/07/03)
- Wait to put the antibiotic in until it is cool enough to touch the bottle to your cheek.
- This prevents thermal degradation.
- If you tend to get bubbles appearing in the agar plates after incubating at 37oC:
- Let the media cool more before pouring plates
- "plates that have been stored in the refrigerator should be separated on a bench top and allowed to warm gradually to room temperature for several hours before incubating at 37 °C." -- Sturdier 2014
Drew Endy has all the basics here