Koch Lab

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Welcome to the KochLab wiki!

We are a research lab at the University of New Mexico, in the Center for High Technology Materials (CHTM) and Physics Department. We are experimental biophysicists, which to us means we use our skills and talents as physical scientists to provide new answers for important questions in cellular and molecular biology. For example, developing new techniques to probe protein-DNA interactions by unzipping single DNA molecules with optical tweezers. You can learn more about our interests on our Research page.

In addition, we are committed to being open scientists and sharing our results, methods, and ideas as quickly, clearly, and openly as possible. However, being a very new lab, we are still learning how to do this and are well behind the curve set by others at OpenWetWare. Therefore, we welcome any requests for information or improvements to add to the wiki, see our Contact page.

We're all over the internet

You can follow our latest endeavors here on OWW or you can follow us in other ways too. Check us out:

KochLab Research... our lab research blog
KochLab Youtube... awesome science videos
Steve's science blog, Steve's research blog, Steve's teaching blog
Flavors of Kochlab... compiling the best of the web

Recent updates to the lab wiki

List of abbreviations:

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5 June 2023

19:44

Upload log Xinyu Liu talk contribs uploaded File:AS2023.jpg ‎

19:41

Gao lab:Publications‎ diffhist +400‎ Xinyu Liu talk contribs (→‎Publications)

4 June 2023

m   07:44

User:Richard J. Abbott‎ diffhist +8‎ Richard J. Abbott talk contribs (→‎Publications)

3 June 2023

			23:44		Lee:JC‎‎ 6 changes history +26‎ [Wooin Lee‎ (6×)]
					23:44 (cur | prev) +9‎ Wooin Lee talk contribs (→‎Schedule)
					23:23 (cur | prev) −10‎ Wooin Lee talk contribs (→‎Schedule)
					23:22 (cur | prev) +6‎ Wooin Lee talk contribs (→‎Schedule)
					23:21 (cur | prev) +7‎ Wooin Lee talk contribs (→‎Schedule)
					23:01 (cur | prev) +7‎ Wooin Lee talk contribs (→‎Schedule)
					22:53 (cur | prev) +7‎ Wooin Lee talk contribs (→‎Schedule)

1 June 2023

			23:23		Lee:Lab Members‎‎ 4 changes history +169‎ [Wooin Lee‎ (4×)]
					23:23 (cur | prev) +164‎ Wooin Lee talk contribs (→‎Research rotation/internship students (past))
					23:21 (cur | prev) +26‎ Wooin Lee talk contribs (→‎Graduate students)
					23:20 (cur | prev) +31‎ Wooin Lee talk contribs (→‎Alumni)
					23:19 (cur | prev) −52‎ Wooin Lee talk contribs (→‎Graduate students)

17:46

BioMicroCenter:Pricing‎ diffhist −79‎ Stuart S. Levine talk contribs (→‎SHORT READ LIBRARY - RNA)

			09:04		(Upload log)‎ [Elizabeth Suesca‎ (2×)]
					09:04 Elizabeth Suesca talk contribs uploaded File:Cloroformo.png ‎
					08:25 Elizabeth Suesca talk contribs uploaded File:Acetato.png ‎

31 May 2023

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UA Biophysics:Protocols:Carotenoid Extration ESP‎ diffhist +423‎ Elizabeth Suesca talk contribs (Created page with "==Materiales para 6 gramos de célula== * Preparar centrífuga a 4 grados * 200 ml Metanol. Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * 500 NaCl 1,7 M (99.348 g/1 L) * 200 Acetato de etilo (Mejor si esta destilado ya que tiende a ser muy hidrófilo) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Cloroformo")

14:24

UA Biophysics:Protocols:K2HPO4 KH2PO4 Buffer‎ diffhist +54‎ Elizabeth Suesca talk contribs

14:23

UA Biophysics:Protocols‎ diffhist −7‎ Elizabeth Suesca talk contribs (→‎BUFFERS)

			N    14:22		UA Biophysics:Protocols:Elution Buffer‎‎ 2 changes history +1,055‎ [Elizabeth Suesca‎ (2×)]
					14:22 (cur | prev) +467‎ Elizabeth Suesca talk contribs
			N		14:07 (cur | prev) +588‎ Elizabeth Suesca talk contribs (Created page with " '''Buffer HEPES: 2 L a 400 mOsM pH 7.4 ==Materiales== MW (g/mol) [] mM mOsM M HEPES 238.30 20 20 9.532 g NaCl 58.44 170 340 19.869 g NaOH (1 M) 8 8 16 ml NaCl 16 32 1.870 g Colocar 1.6 litros de agua deionizada en la botella Pesar el HEPES y el NaCl, diluir en los 1,6 L de dH2O Ajustar pH usando NaOH a 7.4. Calcular la osmolaridad y completar los 400 mOsm con NaCl Completar los dos litros UA Biop...")

			N    14:03		UA Biophysics:Protocols:Calcein ESP‎‎ 3 changes history +1,249‎ [Elizabeth Suesca‎ (3×)]
					14:03 (cur | prev) +43‎ Elizabeth Suesca talk contribs (→‎Procedimiento:)
					14:02 (cur | prev) +63‎ Elizabeth Suesca talk contribs
			N		14:00 (cur | prev) +1,143‎ Elizabeth Suesca talk contribs (Created page with "'''Calceina: 400 mOsM, 50 mM, 100 ml, pH 7.4 ''' ==Materiales: == * Probeta 100 ml * 30 ml de NaOH (1M) * HCl (1M) * 3,113 g de calceina * 40 ml Buffer H: ** 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) ** HEPES 238.3 mg (para que quede a 10 mM en la solución final). ==Procedimiento: == # En la probeta medir 20 ml de NaOH 1M y colocar agitador # Disolver en el NaOH 3,113 g de calceina # Agregar los 40 ml del Buffer H a la calceina # A...")

12:48

BioMicroCenter:QC‎ diffhist −421‎ Stuart S. Levine talk contribs (→‎AGILENT BIOANALYZER 2100)

			08:34		UA Biophysics:Protocols:Calcein‎‎ 2 changes history −751‎ [Elizabeth Suesca‎ (2×)]
					08:34 (cur | prev) +53‎ Elizabeth Suesca talk contribs
					08:33 (cur | prev) −804‎ Elizabeth Suesca talk contribs (Replaced content with " Calcein_ESP<br>") Tag: Replaced

N    08:31

UA Biophysics:Protocols:Bacteria for Lipid Extraction ESP‎ diffhist +1,206‎ Elizabeth Suesca talk contribs (Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo...")

06:42

UA Biophysics:Protocols:Lipid Extraction‎ diffhist −9‎ Elizabeth Suesca talk contribs

30 May 2023

23:52

Hexlab‎ diffhist +440‎ Ximiao He talk contribs

20:13

Holcombe:EyeTrackers‎ diffhist −1‎ Alex O. Holcombe talk contribs (→‎Eyelink 1000 (scattered around the department))

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