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- 13:58, 12 February 2025 Schumer lab: HSV transduction (hist | edit) [3,084 bytes] Schumer (talk | contribs) (Created page with "HSV transduction in Xiphophorus primary embryo cells Last updated April 2024 A few days before transduction: Passage cells into the plates/flask you’d like to use and maintain until 70-80% confluency. Make sure to passage at least 24 hours prior to transduction to avoid stressing the cells as much as possible. I will try to seed around 100k cells in a few wells of a 12 well plate and let them grow for a few days before infection Day of transduction: 1. Treat cell...")
- 13:49, 12 February 2025 Schumer lab: Electroporation (hist | edit) [588 bytes] Schumer (talk | contribs) (Created page with "To electroporate using 100 uL tips: Dissociate and count 600k cells. Pellet and resuspend in 120 uL buffer R. Add 2.4 ug plasmid and mix. Draw up 100uL in the electroporation tip, being careful to avoid bubbles. (Bubbles will cause electrical arcs and kill the cells) This allows for 20 uL overage so that 500k cells and 2 ug plasmid to be in the tip. Electroporate at 1400v, 20ms and 2 pulses. Dispense tip into a flask with regular media + ROCKi. Change media the next d...")
- 18:37, 22 January 2025 BioMicroCenter:PricingFY2025b (hist | edit) [28,526 bytes] Stuart S. Levine (talk | contribs) (Created page with "{{BioMicroCenter}} == SEQUENCING == Please note that samples submitted from '''CORE LAB'''s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR> === NOVASEQ SEQUENCING === {| class="wikitable" border=1 ! NOVASEQ SEQUENCING !width=80| CORE LAB !width=80| MIT !width=80| Non-MIT !width=80| unit ! Notes |...")
- 16:09, 8 January 2025 BIOL388/S25:Week 15 (hist | edit) [83 bytes] Kam D. Dahlquist (talk | contribs) (template and placeholder)
- 16:09, 8 January 2025 BIOL388/S25:Week 14 (hist | edit) [57 bytes] Kam D. Dahlquist (talk | contribs) (template and placeholder)
- 16:08, 8 January 2025 BIOL388/S25:Week 12 (hist | edit) [51 bytes] Kam D. Dahlquist (talk | contribs) (template and placeholder)
- 16:07, 8 January 2025 BIOL388/S25:Week 11 (hist | edit) [70 bytes] Kam D. Dahlquist (talk | contribs) (template and placeholder)
- 16:07, 8 January 2025 BIOL388/S25:Week 10 (hist | edit) [70 bytes] Kam D. Dahlquist (talk | contribs) (template and placeholder)
- 16:06, 8 January 2025 BIOL388/S25:Week 9 (hist | edit) [63 bytes] Kam D. Dahlquist (talk | contribs) (template and placeholder)
- 16:06, 8 January 2025 BIOL388/S25:Week 8 (hist | edit) [54 bytes] Kam D. Dahlquist (talk | contribs) (template and placeholder)
- 16:05, 8 January 2025 BIOL388/S25:Week 6 (hist | edit) [75 bytes] Kam D. Dahlquist (talk | contribs) (template and placeholder)
- 16:05, 8 January 2025 BIOL388/S25:Week 5 (hist | edit) [54 bytes] Kam D. Dahlquist (talk | contribs) (template and placeholder)
- 16:04, 8 January 2025 BIOL388/S25:Week 4 (hist | edit) [212 bytes] Kam D. Dahlquist (talk | contribs) (template and placeholder)
- 16:02, 8 January 2025 BIOL388/S25:Week 3 (hist | edit) [212 bytes] Kam D. Dahlquist (talk | contribs) (templates and placeholder)
- 16:00, 8 January 2025 BIOL388/S25:Week 2 (hist | edit) [6,792 bytes] Kam D. Dahlquist (talk | contribs) (templates and placeholder for assignment)
- 15:38, 8 January 2025 BIOL388/S25:Week 1 (hist | edit) [19,006 bytes] Kam D. Dahlquist (talk | contribs) (add under construction flag)
- 13:30, 7 January 2025 BIOL388/S25:Help (hist | edit) [4,317 bytes] Kam D. Dahlquist (talk | contribs) (begin page with under construction and help)
- 13:29, 7 January 2025 BIOL388/S25:People (hist | edit) [390 bytes] Kam D. Dahlquist (talk | contribs) (begin page with under construction and template)
- 11:18, 6 January 2025 BIOL388/S25 (hist | edit) [25,207 bytes] Kam D. Dahlquist (talk | contribs) (add under construction flag)
