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- 14:34, 14 February 2024 UA Biophysics: Extruder Cleaning Instructons ESP (hist | edit) [601 bytes] Elizabeth Suesca (talk | contribs) (Created page with "Tenga en cuenta que nunca se deben sonicar los émbolos. #Enjuagar con abundante agua los tambores, jeringas y el cilindro metálico #Sonicar con agua desionizada los tambores (10 minutos), si nota que el agua sale muy verde, cambie el agua y sonique nuevamente. #Sonicar con etanol 95% los tambores (10 minutos), #Purgar 10 veces las jeringas conectadas a los tambores con agua desionizada y 10 veces más con alcohol al 95%. #En caso de que no se logre limpiar por comple...")
- 08:54, 12 February 2024 DropBase:sorter and FF devices (hist | edit) [416 bytes] Florian Hollfelder (talk | contribs) (Created page with "=Overview= '''Description''' Flow focussing chip and dielectrophoretic sorter <br> '''Reference''' Nikolic, N., Anagnostidis, V., Tiwari, A., Chait, R. & Gielen, F. Investigating bacteria-phage interaction dynamics using droplet-based technology. BioRxiv, doi:10.1101/2023.07.14.549014 (2023) <br> <br>")
- 12:08, 6 February 2024 Misb23 (hist | edit) [24,700 bytes] Pablo Carbonell (talk | contribs) (Created page with "= Computational Enzyme Design: A Practical Exercise = Pablo Carbonell, [https://www.upv.es/ Universitat Politècnica de València] == Objectives == In this exercise, you will learn about bioinformatics tools and databases that can guide you in order to perform an ''in silico'' protein design. New tools are continuously becoming available. It is advisable, thus, to check always for the latest developments. For instance, take a look [http://www.ncbi.nlm.nih.gov/pubmed?t...")
- 12:48, 5 February 2024 McClean: Tester Protocol (hist | edit) [8,210 bytes] Megan N McClean (talk | contribs) (Created page with "# **EMSA Protocol** **Overview** This is a protocol for the LightShift Chemiluminescent EMSA Kit from Thermo Scientific (cat # 20148). It is highly recommended to read the instructions that come with the kit. They have a lot of good information that will help with decision making. **Materials** - LightShift EMSA Optimization and Control Kit (20148X) - Chemiluminescent Nucleic Acid Detection Module (89880) - Biotin 3' or 5' end-labeled DNA target - Unlabeled DNA targ...")
- 08:50, 28 January 2024 Checking the RNA integrity on gel electrophoresis (hist | edit) [1,705 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "===Preparation of the gel=== * Prepare a 1.5-2% gel electrophoresis. * For example, for a small gel, add 0.53 g agarose and 35 ml TBE/TAE to an Erlenmeyer flask. * Microwave for 30-45 seconds, stop and swirl, and then continue towards a boil until the agarose is completely dissolved. * Let the agarose solution cool down and add 3 µl of 'redsafe' (the amount depends on the 'redsafe' company). * Pour the agarose into a gel tray with the well comb in place. * Use the s...")
- 08:39, 28 January 2024 How to interpret nano-drop results (hist | edit) [786 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "===260/280 Ratio=== * The ratio of absorbance at 260 nm and 280 nm is used to assess the purity of DNA and RNA. * A ratio of ~2.0 is generally accepted as 'pure' for RNA. * If the ratio is appreciably lower in either case, it may indicate the presence of protein, phenol, or other contaminants that absorb strongly at or near 280 nm. ===260/230 Ratio=== *This ratio is used as a secondary measure of nucleic acid purity. * Expected 260/230 values are commonly in the ra...")
- 04:36, 19 December 2023 Analysis LCMS results (hist | edit) [317 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "* Workflow for analysis using MetaboAnalyst [https://www.dropbox.com/scl/fi/7tcri1zvj5zlbaf5u2xjr/Metaboanalyst-workflow.docx?rlkey=vf2b9771nic2dgmjnnsx70ymm&dl=0] * Information about MetaboAnalyst [https://www.dropbox.com/scl/fi/w8ldsd44jod8ahbxksftc/MEtaboanalyst-5.pdf?rlkey=jbhtvlxy2fp22msfcpgxpjfmz&dl=0]")
- 04:24, 19 December 2023 Analysing NGS (hist | edit) [125 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "* R script [https://www.dropbox.com/scl/fi/jyndvcutv5p3wkrx9yr6v/Itai_Dada2_unite.R?rlkey=pgdvz039mjhvsocprx91vs469&dl=0]")
- 04:02, 19 December 2023 How to interoperate nano-drop results (hist | edit) [148 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "[https://www.dropbox.com/scl/fi/38zhpeuyzf4m6dfbd0b86/InterpretingSpecNanodrop.pdf?rlkey=cgb5t71cwgqqcaxxrksie2seu&dl=0]")