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- 12:01, 9 September 2024 UA Biophysics:Protocols:SCD Aminoacids (10x) 1L (hist | edit) [393 bytes] Elizabeth Suesca (talk | contribs) (Created page with "'''Specific amino acids and supplements (10X) 1L''' * 900 mL distilled water * L-Histidine: 200 mg * L-Leucine: 300 mg * L-Lysine: 300 mg * L-Methionine: 200 mg * L-Phenylalanine: 500 mg * L-Threonine: 2 g * L-Tryptophan: 200 mg * L-Tyrosine: 300 mg * L-Valine: 1.5 g * Uracil: 200 mg Fill the bottle with MilliQ until 1 L Store at 4°C Return to Protocols<br>")
- 01:11, 9 September 2024 Prepering a standard curve to quantify the total yeast (hist | edit) [375 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "Image:Standarde_curve.png")
- 00:57, 9 September 2024 Preforming the PCR (hist | edit) [1,047 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "===Perform qPCR with 2X Hy-SYBR power mix (using a different kit requires protocol adjustments) on a 96-well plate=== * Prepare a separate mix for each primer you are using. The SYBR mix is very viscous, so always prepare extra mix (~ x1.2) and mix well * Primers for total yeast quantification: YEASTF (5-GAGTCGAGTTGTTTGGGAATGC-3); YEASTR (5-TCTCTTTCCAAAGTTCTTTTCATCTT T-3)[https://www.dropbox.com/scl/fi/f0khsskz124gi5m7tvenu/Real-Time-Quantitative-PCR.pdf?rlkey=nd0jyn8...")
- 23:47, 8 September 2024 Preparing the plate (hist | edit) [496 bytes] Insect Nutrition and Metabolism (talk | contribs) (Created page with "* First, plan your plate, then prepare your SYBR-primer mix, and dilute your DNA accordingly. * An example for a planned plate: Usually three technical triplicates are used.")
- 09:46, 1 August 2024 UA Biophysics: Sonicador ESP (hist | edit) [1,468 bytes] Elizabeth Suesca (talk | contribs) (Created page with "'''Si no tiene autorización para usarlo por favor comunicarse con biofisica@uniandes.edu.co <Br><Br> Al momento de usar este equipo verifique el estado en que lo encuentra. Si el equipo no está en condiciones óptimas debe reportarlo Y ABSTENERSE DE USARLO. ''' <h2>OPERACIÓN</h2> #Llene el reservorio con agua desionizada. <span style="color:red">Nunca llene por encima de la línea de operación.</span> #Encienda el switch principal que está localizado al lado de...")
- 09:26, 1 August 2024 UA Biophysics: Water Purification System ESP (hist | edit) [1,091 bytes] Elizabeth Suesca (talk | contribs) (Created page with "'''Si no tiene autorización para usarlo por favor comunicarse con biofisica@uniandes.edu.co <Br> Al momento de usar este equipo verifique el estado en que lo encuentra. Si el equipo no está en condiciones óptimas debe reportarlo Y ABSTENERSE DE USARLO. ''' <h2>DISPENSAR </h2> #Remueva la tapa y coloque el recipiente. #Oprima el botón “DISP” #Presiones “DISP” (RO, destilada) o “FLUSH” (UP, desionizada) para seleccionar la calidad del agua. Asegúrese q...")
- 08:07, 1 August 2024 UA Biophysics: Cabinet Biosafety ESP (hist | edit) [1,733 bytes] Elizabeth Suesca (talk | contribs) (Created page with " '''Si no tiene autorización para usarlo por favor comunicarse con biofisica@uniandes.edu.co <Br> Al momento de usar este equipo verifique el estado en que lo encuentra. Si el equipo no está en condiciones óptimas debe reportarlo Y ABSTENERSE DE USARLO. ''' <h2>PREPARACIÓN</h2> #Lentamente levante la compuerta hasta que quede entre las marcas ubicadas al costado izquierdo del área de trabajo. En la pantalla aparecerá un anuncio de espera “WAIT” #Revise que...")
- 12:42, 26 July 2024 UA Biophysics: Analytical Balance Instructions ESP (hist | edit) [1,106 bytes] Elizabeth Suesca (talk | contribs) (Created page with "'''Si no tiene autorización para usarlo por favor comunicarse con biofisica@uniandes.edu.co''' '''Al momento de usar este equipo verifique el estado en que lo encuentra. Si el equipo no está en condiciones óptimas debe reportarlo y abstenerse de usarlo. ''' <h2>Uso de la balanza</h2> #La balanza solo debe ser usada para pesar materiales solidos o líquidos en recientes bien cerrados. #Esta balanza no se usará para pesar medios de cultivo celular. #El peso (materi...")
- 09:28, 26 July 2024 UA Biophysics:Protocols:YPD ESP (hist | edit) [476 bytes] Elizabeth Suesca (talk | contribs) (Created page with "'''YPD 1L''' ''' Materiales:''' *10 G de extracto de levadura *20 G de peptona *Si hace placas, agregue 20 g de agar *Solución de glucosa filtrada al 40% p/v (20g en 50 ml de agua destilada, caliente para diluir). '''Protocolo:''' *Mezcle los ingredientes secos y agregue agua destilada hasta 950 ml *Autoclavar *Añadir los 50 ml de la solución de glucosa para obtener una concentración final de 2% (p/v). Return to Protocols<br>")
- 09:20, 26 July 2024 UA Biophysics:Protocols:YPD 1L (hist | edit) [486 bytes] Elizabeth Suesca (talk | contribs) (Created page with " <br> YPD_ESP<br> Return to Protocols<br>")
- 08:34, 26 July 2024 UA Biophysics:Protocols:Glass Cleaning ESP (hist | edit) [1,259 bytes] Elizabeth Suesca (talk | contribs) (Created page with "Limpieza laminillas con Solución piraña '''Materiales:''' • Ácido sulfúrico H2SO4 • Peróxido H2O2 al 50% • Isopropanol '''Procedimiento:''' '''Día 1:''' En cabina de extracción con guantes de nitrilo de alto calibre: *En un vaso de 25 ml de vidrio preparar 20 ml de Solución H2SO4: H2O2 al 30% agregando el peróxido al agua (8 ml de agua y 12 ml de peróxido). *En vaso de 250 ml colocar 60 ml de Ácido sulfúrico *Se le agregan los 20 ml del peróxido m...")
- 08:28, 26 July 2024 UA Biophysics:Protocols: Glass Cleaning (hist | edit) [1,271 bytes] Elizabeth Suesca (talk | contribs) (Created page with "== Materials == == Protocol == *The samples are dissolve in a mixture of chloroform/methanol/water (3:6:1 v/v):<br> Add chloroform, vortex 5 minutes<br> Add methanol vortex 5 minutes<br> Add water vortex 1 minute<br> and then vortexed every 15min for 4h(solution have to be homogeneous<br> *The samples are then allowed to stand for 2 days to separate the three phases, after which the lower phase of chloroform and lipid is drawn off by aspiration and collected in a clean...")
- 11:59, 20 June 2024 Karas Lab:FREEZ Module (hist | edit) [519 bytes] Aclesage (talk | contribs) (Created page with "{{Karas_Lab}} <div style="padding: 8px; color: #000000; background-color: #ffffff; width: 900px; border: 2px solid #666666;"> =Fragility Processing Pipeline= ==Preface== First things first: if you don't have RAVE installed, follow these steps to do so: RAVE:Install Next, you'll have to pre-process your patient for RAVE. Tutorials for that are available here: RAVE:ravetutorials ==Pre-Processing the Patient for Fragility==")
