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- 08:54, 25 January 2023 BioMicroCenter:PricingFY2023b (hist | edit) [25,442 bytes] Stuart S. Levine (talk | contribs) (Created page with "''' PRICING UPDATE 2/1/2023 ''' == SEQUENCING == Please note that samples submitted from '''CORE LAB'''s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR> === NOVASEQ SEQUENCING === {| class="wikitable" border=1 ! NOVASEQ SEQUENCING !width=80| CORE LAB !width=80| MIT !width=80| Non-MIT !width=80| uni...")
- 05:38, 20 January 2023 UA Biophysics: Water Purification System (hist | edit) [1,066 bytes] Elizabeth Suesca (talk | contribs) (Created page with " '''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' <h2>OPERATION</h2> #Remove the cap and place your flask. #Press “DISP” #Press “DISP” (RO, destilled) o “FLUSH” (UP, deionized) to choose the water quality. Ensure that the pure water at lea...")
- 16:31, 19 January 2023 Dropbase: spinDrop picoinjector (hist | edit) [917 bytes] Florian Hollfelder (talk | contribs) (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 800px|left '''Description''' Blue colour denotes fluidic channels, black – channels for ground electrode, red – signal electrode. Design of the picoinjector device, used to inject the RT enzyme mix. 1) Input channel for droplet reinjection, 2) emulsion diluting oil inlet, 3) droplet spacing oil inlet, 4) inlet for the RT mix to be picoinject...")
- 16:09, 19 January 2023 Dropbase: spinDrop sorter (hist | edit) [1,238 bytes] Florian Hollfelder (talk | contribs) (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 400px|left '''Description''' Blue colour denotes fluidic channels, black – channels for ground electrode, red – signal electrode and the green colour indicates auxiliary channels for insertion of optical fibres. Design of the integrated device used for barcoded bead and single-cell coencapsulation followed by fluorescence-activated droplet sorting...")
- 13:18, 17 January 2023 UA Biophysics: Spectrophotometer (hist | edit) [1,233 bytes] Elizabeth Suesca (talk | contribs) (Created page with "'''To get authorization to use it, please send an email to [mailto:biofisica@uniandes.edu.co Lab manager].''' '''Before beginning any work on the equipment, make sure it is in good condition. If something does not seem right, should be reported to the lab manager. ''' <h2>OPERATION</h2> #The cell holder have to be empty before turning on the instrument. #Turn on the instrument using the power switch on the back panel. #Wait almost 20 min before using it. Spectrometer...")
- 08:46, 16 January 2023 JCAOligoTutorial2b prompt (hist | edit) [1,669 bytes] JCAnderson (talk | contribs) (Created page with "<pre>I am trying to understand a tutorial about the recombinant DNA technique SOEing. The technique is used to silently remove a restriction site when cloning a gene to make a BioBrick part. The experiment involves design of 2 oligos to PCR the region up to the restriction site, and then 2 oligos to amplify the region after the site. Those two PCRs are joined in a SOEing reaction to make a new PCR product without the restriction site. This is then then digested with...")
- 08:31, 16 January 2023 JCAOligoTutorial1b prompt (hist | edit) [943 bytes] JCAnderson (talk | contribs) (Created page with "<code>I am trying to learn recombinant DNA technology. The experiment involves designing 2 oligonucleotides composed of a 5 bp 5' tail of arbitrary sequence, a restriction site polylinker, and the annealing region to amplify a template sequence. I then need to document my experiment in this format: "PCR ca1067F/ca1067R on pSB1AK3-b0015 (1054bp, pcrpdt) Digest pcrpdt (EcoRI/SpeI, 10+1038+6, 1, pcrdig) Digest pSB1A2-I13521 (EcoRI...")
- 15:19, 9 January 2023 DropBase:agarose bead generator (hist | edit) [644 bytes] Florian Hollfelder (talk | contribs) (Created page with " {{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 220px|left '''Description''' Chip for encapsulation of single cells in BG-agarose beads <br> '''Reference''' K. Fischer et al,<i>unpublished</i>, 2023 Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies. '''Designed by''': Tomasz Ka...")
- 15:12, 9 January 2023 Dropbase: agarose bead generator (hist | edit) [651 bytes] Florian Hollfelder (talk | contribs) (Created page with " {{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 180px|left '''Description''' Chip for encapsulation of single cells in BG-agarose beads <br> '''Reference''' K. Fischer et al, bioRxiv, 2023 Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies. <i>unpublished</i> '''Designed by''': T...")
- 15:07, 9 January 2023 Dropbase: 35um bead generator (hist | edit) [650 bytes] Florian Hollfelder (talk | contribs) (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 180px|left '''Description''' Chip for encapsulation of single cells in BG-agarose beads <br> '''Reference''' K. Fischer et al, bioRxiv, 2023 Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies. <i>unpublished</i> '''Designed by''': To...")
- 14:32, 9 January 2023 Dropbase: DEVICE (hist | edit) [0 bytes] Florian Hollfelder (talk | contribs) (Created page with "{{DropBase}} Category:Protocol Category:Microfluidics <div ...> =Overview= 180px|left '''Description''' Microfluidic chip device for fluorescence-activated droplet sorting (FADS) of ≈ 3 pL-sized droplets (offline-incubation). Blue: silanised area for emulsion handling, depth: 21.5–23 μm. Red: signal electrode (-). Black: ground electrode (+). <br> '''Reference''' D. Schnettler Fernández, O. J. Klein, T. S. Kamin...")
- 12:14, 19 December 2022 RAVE:fragility:fragility:output currentlyloadedtrials (hist | edit) [438 bytes] Zhou.oliverfr (talk | contribs) (Created page with "==Currently Loaded Trials== This box shows which trials you currently have loaded.")
- 10:00, 9 December 2022 Griffin:Proteomics (hist | edit) [527 bytes] Korey Griffin (talk | contribs) (Created page with " ==Single Cell Proteomics== *[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643348/ Single Cell ProtEomics (SCoPE2)] *[https://pubmed.ncbi.nlm.nih.gov/34716448/#&gid=article-figures&pid=fig-1-uid-1 SCoPE2 workflow]")
- 08:55, 2 December 2022 RAVE:realtime (hist | edit) [358 bytes] Michael S Beauchamp (talk | contribs) (Created page with "{{RAVE_Navigation_Bar}}")
- 13:18, 13 November 2022 Juan Sebastian Osorio Vivas (hist | edit) [646 bytes] Js.osoriov (talk | contribs) (Created page with ".")
- 12:49, 13 November 2022 UA Biophysics:Protocols:K2HPO4 KH2PO4 Buffer (hist | edit) [517 bytes] Js.osoriov (talk | contribs) (Created page with "For the preparation of 1 liter of potassium phosphate solution the final desired concentrations are: *0.17 Molar monobasic potassium phospate (23.135 grams/liter KH2PO4, molecular weight=136.09 grams/mole) *0.72 Molar dibasic potassium phosphate (125.410 grams/liter K2HPO4, molecular weight=174.18 grams/mole). Add the dry ingredients to a 1 liter graduated cylinder and added double distilled water until a final volume of 1 liter is reached. Sterile filter this solution...")
- 12:46, 13 November 2022 UA Biophysics:Protocols:TB 1L (hist | edit) [600 bytes] Js.osoriov (talk | contribs) (Created page with "'''Terrific broth (TB, not to be confused with T Broth)''' == Ingredients == #12 g yryptone. #24 g yeast extract. #4 mL Glycerol. #100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, to be prepared separately from the tryptone, yeast extract, and glycerol solution. ==Protocol== #Mix tryptone, yeast extract and glycerol, and add distilled water up to 900mL. #Pour into 1 L flask (or greater). '''Autoclave ''' #Allow liquid to cool to less than 60 degrees Centrigrade. #Add 100...")
- 07:13, 9 November 2022 Beauchamp:PennMcGurkBattery (hist | edit) [2,286 bytes] Michael S Beauchamp (talk | contribs) (Created page with "{{Beauchamp Navigation Bar}} 50 px|link=https://creativecommons.org/publicdomain/zero/1.0/ [https://creativecommons.org/publicdomain/zero/1.0/ To the extent possible under law, we waive all copyright and related or neighboring rights to the materials on this page.] (Of course, we appreciate citations to the relevant papers.) ===Penn McGurk Battery=== To make it easier to study the McGurk effect, the Beauchamp Lab has released the Penn McGurk Battery...")
- 14:47, 30 October 2022 Y3 BME Automation Group Project : References (hist | edit) [6,103 bytes] Jyf20 (talk | contribs) (Created page with "== References == === Generic Automation === *Jessop-Fabre, M.M. and Sonnenschein, N. (2019) ‘Improving Reproducibility in Synthetic Biology’, Frontiers in Bioengineering and Biotechnology, 7, p. 18. Available at: https://doi.org/10.3389/fbioe.2019.00018. *Manyika, J. et al. (2017) A future that works: Automation, employment, and productivity, Mckinsey & Company. Available at: https://www.mckinsey.com/featured-insights/digital-disruption/harnessing-automation-for-a-...")
