User contributions for Elizabeth Suesca

26 June 2023

• 13:1913:19, 26 June 2023 diff hist +197‎ UA Biophysics:Protocols:Carotenoid Extration ‎No edit summary current
• 13:1613:16, 26 June 2023 diff hist +40‎ UA Biophysics:Protocols:Lipid Extration ESP ‎ →‎Procedimiento current
• 13:1513:15, 26 June 2023 diff hist +1,529‎ N UA Biophysics:Protocols:Lipid Extration ESP ‎ Created page with "==Materials for 1 g of Cell== * Células SA401 pesadas y maceradas con mortero * Cloroformo * Metanol Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * Perlas de vidrio * Solución de NaCl 1.7 M (10% W/V) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Fascos de vidrio de 50 ml '''Nota:''' Preparar una mezcla de cloroformo:metanol (2:1) (20 ml de cloroformo/10 m..."
• 13:1513:15, 26 June 2023 diff hist −1,453‎ UA Biophysics:Protocols:Lipid Extraction ‎No edit summary current
• 13:1213:12, 26 June 2023 diff hist +161‎ UA Biophysics:Protocols:Lipid Extraction ‎ →‎Procedimiento
• 12:4712:47, 26 June 2023 diff hist +149‎ UA Biophysics:Protocols:Lipid Extraction ‎No edit summary
• 08:2108:21, 26 June 2023 diff hist +855‎ UA Biophysics:Protocols:Lipid Extraction ‎No edit summary

1 June 2023

• 09:0609:06, 1 June 2023 diff hist +53‎ UA Biophysics:Protocols:Carotenoid Extration ESP ‎No edit summary current
• 09:0509:05, 1 June 2023 diff hist +59‎ UA Biophysics:Protocols:Carotenoid Extration ESP ‎No edit summary
• 09:0409:04, 1 June 2023 diff hist 0‎ N File:Cloroformo.png ‎No edit summary current
• 09:0409:04, 1 June 2023 diff hist +474‎ UA Biophysics:Protocols:Carotenoid Extration ESP ‎No edit summary
• 08:4308:43, 1 June 2023 diff hist +781‎ UA Biophysics:Protocols:Carotenoid Extration ESP ‎ →‎Procedimiento
• 08:2508:25, 1 June 2023 diff hist 0‎ N File:Acetato.png ‎No edit summary current
• 08:2508:25, 1 June 2023 diff hist +1,023‎ UA Biophysics:Protocols:Carotenoid Extration ESP ‎ →‎Materiales para 6 gramos de célula

31 May 2023

• 14:3914:39, 31 May 2023 diff hist +423‎ N UA Biophysics:Protocols:Carotenoid Extration ESP ‎ Created page with "==Materiales para 6 gramos de célula== * Preparar centrífuga a 4 grados * 200 ml Metanol. Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * 500 NaCl 1,7 M (99.348 g/1 L) * 200 Acetato de etilo (Mejor si esta destilado ya que tiende a ser muy hidrófilo) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Cloroformo"
• 14:3414:34, 31 May 2023 diff hist −2‎ UA Biophysics:Protocols:Carotenoid Extration ‎No edit summary
• 14:3414:34, 31 May 2023 diff hist +88‎ UA Biophysics:Protocols:Carotenoid Extration ‎No edit summary
• 14:2414:24, 31 May 2023 diff hist +54‎ UA Biophysics:Protocols:K2HPO4 KH2PO4 Buffer ‎No edit summary current
• 14:2314:23, 31 May 2023 diff hist −7‎ UA Biophysics:Protocols ‎ →‎BUFFERS current
• 14:2214:22, 31 May 2023 diff hist +467‎ UA Biophysics:Protocols:Elution Buffer ‎No edit summary current
• 14:0714:07, 31 May 2023 diff hist +588‎ N UA Biophysics:Protocols:Elution Buffer ‎ Created page with " '''Buffer HEPES: 2 L a 400 mOsM pH 7.4 ==Materiales== MW (g/mol) [] mM mOsM M HEPES 238.30 20 20 9.532 g NaCl 58.44 170 340 19.869 g NaOH (1 M) 8 8 16 ml NaCl 16 32 1.870 g Colocar 1.6 litros de agua deionizada en la botella Pesar el HEPES y el NaCl, diluir en los 1,6 L de dH2O Ajustar pH usando NaOH a 7.4. Calcular la osmolaridad y completar los 400 mOsm con NaCl Completar los dos litros UA Biop..."
• 14:0314:03, 31 May 2023 diff hist +43‎ UA Biophysics:Protocols:Calcein ESP ‎ →‎Procedimiento: current
• 14:0214:02, 31 May 2023 diff hist +63‎ UA Biophysics:Protocols:Calcein ESP ‎No edit summary
• 14:0014:00, 31 May 2023 diff hist +1,143‎ N UA Biophysics:Protocols:Calcein ESP ‎ Created page with "'''Calceina: 400 mOsM, 50 mM, 100 ml, pH 7.4 ''' ==Materiales: == * Probeta 100 ml * 30 ml de NaOH (1M) * HCl (1M) * 3,113 g de calceina * 40 ml Buffer H: ** 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) ** HEPES 238.3 mg (para que quede a 10 mM en la solución final). ==Procedimiento: == # En la probeta medir 20 ml de NaOH 1M y colocar agitador # Disolver en el NaOH 3,113 g de calceina # Agregar los 40 ml del Buffer H a la calceina # A..."
• 08:3408:34, 31 May 2023 diff hist +53‎ UA Biophysics:Protocols:Calcein ‎No edit summary current
• 08:3308:33, 31 May 2023 diff hist −804‎ UA Biophysics:Protocols:Calcein ‎ Replaced content with " Calcein_ESP<br>" Tag: Replaced
• 08:3208:32, 31 May 2023 diff hist +3‎ UA Biophysics:Protocols:Bacteria for Lipid Extraction ‎No edit summary current
• 08:3108:31, 31 May 2023 diff hist +1,206‎ N UA Biophysics:Protocols:Bacteria for Lipid Extraction ESP ‎ Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo..." current
• 08:3108:31, 31 May 2023 diff hist −1,205‎ UA Biophysics:Protocols:Bacteria for Lipid Extraction ‎No edit summary
• 08:3008:30, 31 May 2023 diff hist +47‎ UA Biophysics:Protocols:Bacteria for Lipid Extraction ‎No edit summary
• 08:2908:29, 31 May 2023 diff hist +106‎ UA Biophysics:Protocols:Bacteria for Lipid Extraction ‎No edit summary
• 06:4206:42, 31 May 2023 diff hist −9‎ UA Biophysics:Protocols:Lipid Extraction ‎No edit summary

30 May 2023

• 06:4406:44, 30 May 2023 diff hist +89‎ UA Biophysics:Protocols:Lipid Extraction ‎No edit summary

29 May 2023

• 13:0913:09, 29 May 2023 diff hist +333‎ UA Biophysics:Protocols:Bacteria for Lipid Extraction ‎No edit summary
• 13:0313:03, 29 May 2023 diff hist −4‎ UA Biophysics:Protocols ‎ →‎EXTRACTION Tag: Manual revert
• 13:0213:02, 29 May 2023 diff hist +4‎ UA Biophysics:Protocols ‎ →‎EXTRACTION
• 12:5912:59, 29 May 2023 diff hist −10‎ UA Biophysics:Protocols:Bacteria for Lipid Extraction ‎No edit summary
• 12:5812:58, 29 May 2023 diff hist +53‎ UA Biophysics:Protocols:Bacteria for Lipid Extraction ‎No edit summary
• 12:5712:57, 29 May 2023 diff hist +1,104‎ N UA Biophysics:Protocols:Bacteria for Lipid Extraction ‎ Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo..."
• 12:3012:30, 29 May 2023 diff hist +100‎ UA Biophysics:Protocols ‎ →‎EXTRACTION
• 12:2912:29, 29 May 2023 diff hist +11‎ UA Biophysics:Protocols:Lipid Extraction ‎No edit summary
• 12:2412:24, 29 May 2023 diff hist +2‎ UA Biophysics:Protocols:Erythromycin ‎No edit summary current
• 12:2012:20, 29 May 2023 diff hist +41‎ UA Biophysics:Protocols:Erythromycin ‎No edit summary
• 12:1812:18, 29 May 2023 diff hist +1‎ UA Biophysics:Protocols:Erythromycin ‎ →‎PROTOCOL (STORAGE)
• 12:1712:17, 29 May 2023 diff hist +1,186‎ N UA Biophysics:Protocols:Erythromycin ‎ Created page with "Erythromycin has been used as a motilin receptor agonist, to block respiratory glycoconjugate secretion in human airways in vitro, and for selecting plasmid-cured and recombinant lactococcus lactis MG1363 strains. ==STOCK SOLUTION== Erythromycin is used in the biophysics lab at a 2 ug/ml. Stock solutions are at 1 mg/mL ==PROTOCOL (STORAGE)== According to desired amount of antibiotics, calculate the amount in grams that has to be weighed. For example, for 20mL of 25mg..."
• 12:0912:09, 29 May 2023 diff hist +2‎ UA Biophysics:Protocols ‎ →‎ANTIBIOTICS STOCK
• 12:0912:09, 29 May 2023 diff hist +57‎ UA Biophysics:Protocols ‎ →‎ANTIBIOTICS STOCK
• 11:5811:58, 29 May 2023 diff hist +860‎ N UA Biophysics:Protocols:Calcein ‎ Created page with "Receta para 100 ml, 50mM, 400 mOsM, pH 7,4 Materiales: Probeta 100 ml 30 ml de NaOH 3,113 g de calceina Procedimiento: En probeta 20 ml de NaOH 1M (colocque 21 ml) Disolver en el NaOH 3,113 g de calceina Mezclar 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) y HEPES 238.3 mg (para que quede a 10 mM).completar un volumen de 40 ml Agregar la mezcla a la calceina Ajustar pH con HCl 1M (2 ml) Entre HCl y NaOH se agregaron 2..."
• 08:5608:56, 29 May 2023 diff hist +9‎ UA Biophysics:Protocols: GUV ‎No edit summary current

25 May 2023

• 13:0113:01, 25 May 2023 diff hist +11‎ UA Biophysics:Protocols ‎No edit summary