User contributions for Elizabeth Suesca
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26 June 2023
- 13:1913:19, 26 June 2023 diff hist +197 UA Biophysics:Protocols:Carotenoid Extration No edit summary current
- 13:1613:16, 26 June 2023 diff hist +40 UA Biophysics:Protocols:Lipid Extration ESP →Procedimiento current
- 13:1513:15, 26 June 2023 diff hist +1,529 N UA Biophysics:Protocols:Lipid Extration ESP Created page with "==Materials for 1 g of Cell== * Células SA401 pesadas y maceradas con mortero * Cloroformo * Metanol Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * Perlas de vidrio * Solución de NaCl 1.7 M (10% W/V) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Fascos de vidrio de 50 ml '''Nota:''' Preparar una mezcla de cloroformo:metanol (2:1) (20 ml de cloroformo/10 m..."
- 13:1513:15, 26 June 2023 diff hist −1,453 UA Biophysics:Protocols:Lipid Extraction No edit summary current
- 13:1213:12, 26 June 2023 diff hist +161 UA Biophysics:Protocols:Lipid Extraction →Procedimiento
- 12:4712:47, 26 June 2023 diff hist +149 UA Biophysics:Protocols:Lipid Extraction No edit summary
- 08:2108:21, 26 June 2023 diff hist +855 UA Biophysics:Protocols:Lipid Extraction No edit summary
1 June 2023
- 09:0609:06, 1 June 2023 diff hist +53 UA Biophysics:Protocols:Carotenoid Extration ESP No edit summary current
- 09:0509:05, 1 June 2023 diff hist +59 UA Biophysics:Protocols:Carotenoid Extration ESP No edit summary
- 09:0409:04, 1 June 2023 diff hist 0 N File:Cloroformo.png No edit summary current
- 09:0409:04, 1 June 2023 diff hist +474 UA Biophysics:Protocols:Carotenoid Extration ESP No edit summary
- 08:4308:43, 1 June 2023 diff hist +781 UA Biophysics:Protocols:Carotenoid Extration ESP →Procedimiento
- 08:2508:25, 1 June 2023 diff hist 0 N File:Acetato.png No edit summary current
- 08:2508:25, 1 June 2023 diff hist +1,023 UA Biophysics:Protocols:Carotenoid Extration ESP →Materiales para 6 gramos de célula
31 May 2023
- 14:3914:39, 31 May 2023 diff hist +423 N UA Biophysics:Protocols:Carotenoid Extration ESP Created page with "==Materiales para 6 gramos de célula== * Preparar centrífuga a 4 grados * 200 ml Metanol. Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * 500 NaCl 1,7 M (99.348 g/1 L) * 200 Acetato de etilo (Mejor si esta destilado ya que tiende a ser muy hidrófilo) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Cloroformo"
- 14:3414:34, 31 May 2023 diff hist −2 UA Biophysics:Protocols:Carotenoid Extration No edit summary
- 14:3414:34, 31 May 2023 diff hist +88 UA Biophysics:Protocols:Carotenoid Extration No edit summary
- 14:2414:24, 31 May 2023 diff hist +54 UA Biophysics:Protocols:K2HPO4 KH2PO4 Buffer No edit summary current
- 14:2314:23, 31 May 2023 diff hist −7 UA Biophysics:Protocols →BUFFERS current
- 14:2214:22, 31 May 2023 diff hist +467 UA Biophysics:Protocols:Elution Buffer No edit summary current
- 14:0714:07, 31 May 2023 diff hist +588 N UA Biophysics:Protocols:Elution Buffer Created page with " '''Buffer HEPES: 2 L a 400 mOsM pH 7.4 ==Materiales== MW (g/mol) [] mM mOsM M HEPES 238.30 20 20 9.532 g NaCl 58.44 170 340 19.869 g NaOH (1 M) 8 8 16 ml NaCl 16 32 1.870 g Colocar 1.6 litros de agua deionizada en la botella Pesar el HEPES y el NaCl, diluir en los 1,6 L de dH2O Ajustar pH usando NaOH a 7.4. Calcular la osmolaridad y completar los 400 mOsm con NaCl Completar los dos litros UA Biop..."
- 14:0314:03, 31 May 2023 diff hist +43 UA Biophysics:Protocols:Calcein ESP →Procedimiento: current
- 14:0214:02, 31 May 2023 diff hist +63 UA Biophysics:Protocols:Calcein ESP No edit summary
- 14:0014:00, 31 May 2023 diff hist +1,143 N UA Biophysics:Protocols:Calcein ESP Created page with "'''Calceina: 400 mOsM, 50 mM, 100 ml, pH 7.4 ''' ==Materiales: == * Probeta 100 ml * 30 ml de NaOH (1M) * HCl (1M) * 3,113 g de calceina * 40 ml Buffer H: ** 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) ** HEPES 238.3 mg (para que quede a 10 mM en la solución final). ==Procedimiento: == # En la probeta medir 20 ml de NaOH 1M y colocar agitador # Disolver en el NaOH 3,113 g de calceina # Agregar los 40 ml del Buffer H a la calceina # A..."
- 08:3408:34, 31 May 2023 diff hist +53 UA Biophysics:Protocols:Calcein No edit summary current
- 08:3308:33, 31 May 2023 diff hist −804 UA Biophysics:Protocols:Calcein Replaced content with " Calcein_ESP<br>" Tag: Replaced
- 08:3208:32, 31 May 2023 diff hist +3 UA Biophysics:Protocols:Bacteria for Lipid Extraction No edit summary current
- 08:3108:31, 31 May 2023 diff hist +1,206 N UA Biophysics:Protocols:Bacteria for Lipid Extraction ESP Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo..." current
- 08:3108:31, 31 May 2023 diff hist −1,205 UA Biophysics:Protocols:Bacteria for Lipid Extraction No edit summary
- 08:3008:30, 31 May 2023 diff hist +47 UA Biophysics:Protocols:Bacteria for Lipid Extraction No edit summary
- 08:2908:29, 31 May 2023 diff hist +106 UA Biophysics:Protocols:Bacteria for Lipid Extraction No edit summary
- 06:4206:42, 31 May 2023 diff hist −9 UA Biophysics:Protocols:Lipid Extraction No edit summary
30 May 2023
- 06:4406:44, 30 May 2023 diff hist +89 UA Biophysics:Protocols:Lipid Extraction No edit summary
29 May 2023
- 13:0913:09, 29 May 2023 diff hist +333 UA Biophysics:Protocols:Bacteria for Lipid Extraction No edit summary
- 13:0313:03, 29 May 2023 diff hist −4 UA Biophysics:Protocols →EXTRACTION Tag: Manual revert
- 13:0213:02, 29 May 2023 diff hist +4 UA Biophysics:Protocols →EXTRACTION
- 12:5912:59, 29 May 2023 diff hist −10 UA Biophysics:Protocols:Bacteria for Lipid Extraction No edit summary
- 12:5812:58, 29 May 2023 diff hist +53 UA Biophysics:Protocols:Bacteria for Lipid Extraction No edit summary
- 12:5712:57, 29 May 2023 diff hist +1,104 N UA Biophysics:Protocols:Bacteria for Lipid Extraction Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo..."
- 12:3012:30, 29 May 2023 diff hist +100 UA Biophysics:Protocols →EXTRACTION
- 12:2912:29, 29 May 2023 diff hist +11 UA Biophysics:Protocols:Lipid Extraction No edit summary
- 12:2412:24, 29 May 2023 diff hist +2 UA Biophysics:Protocols:Erythromycin No edit summary current
- 12:2012:20, 29 May 2023 diff hist +41 UA Biophysics:Protocols:Erythromycin No edit summary
- 12:1812:18, 29 May 2023 diff hist +1 UA Biophysics:Protocols:Erythromycin →PROTOCOL (STORAGE)
- 12:1712:17, 29 May 2023 diff hist +1,186 N UA Biophysics:Protocols:Erythromycin Created page with "Erythromycin has been used as a motilin receptor agonist, to block respiratory glycoconjugate secretion in human airways in vitro, and for selecting plasmid-cured and recombinant lactococcus lactis MG1363 strains. ==STOCK SOLUTION== Erythromycin is used in the biophysics lab at a 2 ug/ml. Stock solutions are at 1 mg/mL ==PROTOCOL (STORAGE)== According to desired amount of antibiotics, calculate the amount in grams that has to be weighed. For example, for 20mL of 25mg..."
- 12:0912:09, 29 May 2023 diff hist +2 UA Biophysics:Protocols →ANTIBIOTICS STOCK
- 12:0912:09, 29 May 2023 diff hist +57 UA Biophysics:Protocols →ANTIBIOTICS STOCK
- 11:5811:58, 29 May 2023 diff hist +860 N UA Biophysics:Protocols:Calcein Created page with "Receta para 100 ml, 50mM, 400 mOsM, pH 7,4 Materiales: Probeta 100 ml 30 ml de NaOH 3,113 g de calceina Procedimiento: En probeta 20 ml de NaOH 1M (colocque 21 ml) Disolver en el NaOH 3,113 g de calceina Mezclar 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) y HEPES 238.3 mg (para que quede a 10 mM).completar un volumen de 40 ml Agregar la mezcla a la calceina Ajustar pH con HCl 1M (2 ml) Entre HCl y NaOH se agregaron 2..."
- 08:5608:56, 29 May 2023 diff hist +9 UA Biophysics:Protocols: GUV No edit summary current
25 May 2023
- 13:0113:01, 25 May 2023 diff hist +11 UA Biophysics:Protocols No edit summary