Everything you should really know when to tackle a problem in Synthetic Biology - Expand your knowledge about engineering concepts and biological systems when browsing through our collection of lecture notes
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Do you want to get to know the celebrities working on this project? Click the link above to learn more about all the members of I.CoLi 2006 (Imperial College London iGEM 2006)
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Even more celebrities are involved in this exciting project. Find out more with the link above
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Acknowledgements__HIDER__
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Throughout the project, many people have helped us in various ways - we are very thankful for all their precious time & efforts spent to support iGEM at Imperial ! Special thanks to everyone on the Acknowledgements Page.
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UA Biophysics:Protocols:SCD Aminoacids (10x) 1Ldiffhist+393Elizabeth Suescatalkcontribs(Created page with "'''Specific amino acids and supplements (10X) 1L''' * 900 mL distilled water * L-Histidine: 200 mg * L-Leucine: 300 mg * L-Lysine: 300 mg * L-Methionine: 200 mg * L-Phenylalanine: 500 mg * L-Threonine: 2 g * L-Tryptophan: 200 mg * L-Tyrosine: 300 mg * L-Valine: 1.5 g * Uracil: 200 mg Fill the bottle with MilliQ until 1 L Store at 4°C Return to Protocols<br>")
00:57 (cur | prev) +501 Insect Nutrition and Metabolismtalkcontribs(Created page with "===Perform qPCR with 2X Hy-SYBR power mix (using a different kit requires protocol adjustments) on a 96-well plate=== * Prepare a separate mix for each primer you are using. The SYBR mix is very viscous, so always prepare extra mix (~ x1.2) and mix well * Primers for total yeast quantification: YEASTF (5-GAGTCGAGTTGTTTGGGAATGC-3); YEASTR (5-TCTCTTTCCAAAGTTCTTTTCATCTT T-3)[https://www.dropbox.com/scl/fi/f0khsskz124gi5m7tvenu/Real-Time-Quantitative-PCR.pdf?rlkey=nd0jyn8...")
23:47 (cur | prev) +173 Insect Nutrition and Metabolismtalkcontribs(Created page with "* First, plan your plate, then prepare your SYBR-primer mix, and dilute your DNA accordingly. * An example for a planned plate: Usually three technical triplicates are used.")
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