Difference between revisions of "Gill:Protocols"

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{{Gill}}
 
{{Gill}}
  
===Protocols===
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==Protocols==
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===Microbiology===
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[[Gill:Chung cells |Chung cells]] <br>
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[[Gill:Making frozen permanents |Making frozen permanents]] <br>
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===Solutions and Media Preparation===
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 +
[[Gill:TNA (Tryptone Nutrient Agar) |TNA (Tryptone Nutrient Agar)]]
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[[Gill:TNB (Tryptone Nutrient Broth) |TNB (Tryptone Nutrient Broth)]]
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[[Gill:TNA-SA |TNA-SA]]
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[[Gill:Tris-HCl |Tris-HCl]]
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[[Gill:Lambda diluent (SM buffer) |Lambda diluent (SM buffer)]]
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[[Gill:10x Lambda diluent (no gelatin) |10x Lambda diluent (no gelatin)]]
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[[Gill:TSB (Tryptone Soy Broth) |TSB (Tryptone Soy Broth)]]
 +
 
 +
[[Gill:TSA (Tryptone Soy Agar) |TSA (Tryptone Soy Agar)]]
 +
 
 +
[[Gill:LB broth |LB broth]]
 +
 
 +
[[Gill:LB agar |LB agar]]
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 +
[[Gill:S liquid medium |S liquid medium]]
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 +
[[Gill:T-top (0.5% agar) |T-top (0.5% agar)]]
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[[Gill:10 X M9 salts |10 X M9 salts]]
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[[Gill:10 X M9 salts + glucose |10 X M9 salts + glucose]]
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[[Gill:M9 agar |M9 agar]]
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 +
[[Gill:Supplemented M9 agar (M9sup) |Supplemented M9 agar (M9sup)]]
 +
 
 +
[[Gill:Supplemented M9 broth (M9sup) |Supplemented M9 broth (M9sup)]]
 +
 
 +
[[Gill:Vogel-Bonner glucose (VBG) minimal medium |Vogel-Bonner glucose (VBG) minimal medium]]
 +
 
 +
[[Gill:50X Vogel-Bonner salts |50X Vogel-Bonner salts]]

Revision as of 10:46, 20 August 2013

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Protocols

Microbiology

Chung cells
Making frozen permanents

Solutions and Media Preparation

TNA (Tryptone Nutrient Agar)

TNB (Tryptone Nutrient Broth)

TNA-SA

Tris-HCl

Lambda diluent (SM buffer)

10x Lambda diluent (no gelatin)

TSB (Tryptone Soy Broth)

TSA (Tryptone Soy Agar)

LB broth

LB agar

S liquid medium

T-top (0.5% agar)

10 X M9 salts

10 X M9 salts + glucose

M9 agar

Supplemented M9 agar (M9sup)

Supplemented M9 broth (M9sup)

Vogel-Bonner glucose (VBG) minimal medium

50X Vogel-Bonner salts