Gill:TNB (Tryptone Nutrient Broth)

Materials

500 mL:

• 2.5 g Tryptone
• 1.25 g Yeast extract
• 0.5 g Glucose
• 4.25 g NaCl
• 500 ml dH₂O

Procedure

Mix all ingredients in a 1 litre bottle or flask, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!