Gill:TNB (Tryptone Nutrient Broth)

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500 mL:

  • 2.5 g Tryptone
  • 1.25 g Yeast extract
  • 0.5 g Glucose
  • 4.25 g NaCl
  • 500 ml dH2O


Mix all ingredients in a 1 litre bottle or flask, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!