TNA soft agar, 0.5% agar
Make 500 ml of TNB (Gill:TNB (Tryptone Nutrient Broth)) in a 1 litre bottle or flask. Add 0.5 g agar to 5 small (200-250 ml) bottles and add 100 ml of the TNB to each bottle, then autoclave. Let bottles cool and tighten caps, store at room temp.
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!