Gill:S liquid medium

Overview

For Bcc EPS production.

Materials

1000 mL:

• 1 g Yeast extract
• 10 g Na₂HPO₄
• 3 g KH₂PO₄
• 1 g K₂SO₄
• 1 g NaCl
• 900 mL Water

Procedure

Dissolve above components in 900 ml water and autoclave. Before use, add the following components from sterile stock solutions:

20 % Glucose	100 ml	(2% final)
10 % Casamino acids	10 ml	(0.1% final)
MgSO4 (from 1 M stock)	1 ml	(1 mM final)
CaCl2 (from 1 M stock)	0.1 ml	(0.1 mM final)
FeSO4 (from 0.1 M stock)	50 µl	(5 µM final)

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!