Gill:S liquid medium
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For Bcc EPS production.
- 1 g Yeast extract
- 10 g Na2HPO4
- 3 g KH2PO4
- 1 g K2SO4
- 1 g NaCl
- 900 mL Water
Dissolve above components in 900 ml water and autoclave. Before use, add the following components from sterile stock solutions:
|20 % Glucose||100 ml||(2% final)|
|10 % Casamino acids||10 ml||(0.1% final)|
|MgSO4 (from 1 M stock)||1 ml||(1 mM final)|
|CaCl2 (from 1 M stock)||0.1 ml||(0.1 mM final)|
|FeSO4 (from 0.1 M stock)||50 µl||(5 µM final)|
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!