Gill:Supplemented M9 broth (M9sup)

Materials

	50 mL	500 mL	1000 mL
dH2O	43 ml	430 ml	860 ml
10 X M9 salts + glucose	5 ml	50 ml	100 ml
10% Casamino acids	1 ml	10 ml	20 ml
1% Thiamine	50 µl	0.5 ml	1 ml
1 M MgSO4	100 µl	1 ml	2 ml
1 M CaCl2	5 µl	50 µl	0.1 ml

Procedure

Pre-sterilize all component solutions and aseptically add to sterile water. Alternatively, mix all components and then filter-sterilize through a 0.22 µm filter. Store at 4 ºC.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!