Gill:Lambda diluent (SM buffer)

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1000 mL
1M Tris-HCl, pH 7.5 50 ml (25 mM)
NaCl 5.8 g (100 mM)
MgSO4 1 g (8 mM)
Gelatin (2% sol’n) 5 ml (0.01 %)
dH2O 475 ml


Mix all ingredients, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at room temp.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!