Gill:TNA (Tryptone Nutrient Agar)

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500 mL:

  • 2.5 g Tryptone
  • 1.25 g Yeast extract
  • 0.5 g Glucose
  • 4.25 g NaCl
  • 7.5 g Agar
  • 500 ml dH2O


Mix all ingredients in a 1 litre bottle or flask, cover and autoclave. Label caps with “TNA” to avoid confusion. Immediately after autoclaving, swirl media to dissolve the melted agar. Tighten caps and place bottles in the water bath. Pour plates when the agar temperature is 50-60 ºC.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!