Gill:TNA (Tryptone Nutrient Agar)

Materials

500 mL:

• 2.5 g Tryptone
• 1.25 g Yeast extract
• 0.5 g Glucose
• 4.25 g NaCl
• 7.5 g Agar
• 500 ml dH₂O

Procedure

Mix all ingredients in a 1 litre bottle or flask, cover and autoclave. Label caps with “TNA” to avoid confusion. Immediately after autoclaving, swirl media to dissolve the melted agar. Tighten caps and place bottles in the water bath. Pour plates when the agar temperature is 50-60 ºC.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!