Gill:Making frozen permanents

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  • Cryo vials
  • Overnight bacterial culture
  • LB (50% glycerol)


Pipette 400 μL LB 50% glycerol and 600 μL overnight culture into a cryo vial. Vortex, put in a freezer box and store in the -80 degree freezer.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  • When making LB in 50% glycerol, measure out the glycerol in a graduated cylinder before adding water. To get the last of the glycerol out of the cylinder, add the required amount of water, cover the top with parafilm, and shake before pouring out.