Gill:10x Lambda diluent (no gelatin)

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1000 mL:

  • 60.56 g Tris base
  • 58.0 g NaCl
  • 10 g MgSO4
  • dH2O to 1000 ml


Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Autoclave, store at room temp.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!