Gill:Vogel-Bonner glucose (VBG) minimal medium

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100 mL 500 mL
50X VBG salts 2 ml 10 ml
20% glucose 5 ml 25 ml
Noble agar 2 g 10 g
dH2O 93 ml 465 ml


Add agar to water, autoclave, cool slightly (about 5-10 min at RT); pre-warm 50X VBG salts to 50 – 55 ºC. While gently mixing, slowly aseptically add VBG salts and glucose, mix and pour.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!