Gill:LB broth

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1000 mL:

  • 10 g Bacto tryptone
  • 10 g NaCl (5 g NaCl for LB Lennox)
  • 5 g Yeast extract
  • 1000 ml dH2O


Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!