Gill:LB broth

Materials

1000 mL:

• 10 g Bacto tryptone
• 10 g NaCl (5 g NaCl for LB Lennox)
• 5 g Yeast extract
• 1000 ml dH₂O

Procedure

Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.

Notes

Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!