- 10 g Bacto tryptone
- 10 g NaCl (5 g NaCl for LB Lennox)
- 5 g Yeast extract
- 1000 ml dH2O
Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!