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1M Tris-HCl, pH 7.5


1000 mL:

  • 121.12 g Tris base
  • 800 mL dH2O
  • HCl


Dissolve in 800 ml dH2O, adjust pH to 7.5 with HCl, add water to a final volume of 1000 ml. Aliquot as appropriate, autoclave, store at room temp.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  • If you overshoot the pH, don't add another base. You can add more Tris, but it will change the final concentration.