Gill:TSB (Tryptone Soy Broth)
- 15 g TSB (Bacto)
- 500 ml dH2O
- 30 g TSB (Bacto)
- 1000 ml dH2O
Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.
Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
- Don't tighten caps directly after autoclaving. This creates a vacuum that draws contaminants into the bottle when opened and may crack the glass.