Gill:TSB (Tryptone Soy Broth)

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500 mL:

  • 15 g TSB (Bacto)
  • 500 ml dH2O

1000 mL:

  • 30 g TSB (Bacto)
  • 1000 ml dH2O


Dissolve medium in water, then aliquot 100 ml into square milk bottles & autoclave. Let bottles cool and tighten caps, store at 4 ºC in the cold room.


Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!

  • Don't tighten caps directly after autoclaving. This creates a vacuum that draws contaminants into the bottle when opened and may crack the glass.