Gill:Protocols

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Protocols

Microbiology

Making frozen permanents

Confirming phage via gel electrophoresis

Solutions and Media Preparation

TNA (Tryptone Nutrient Agar)

TNB (Tryptone Nutrient Broth)

TNA-SA

Tris-HCl

Lambda diluent (SM buffer)

10x Lambda diluent (no gelatin)

TSB (Tryptone Soy Broth)

TSA (Tryptone Soy Agar)

LB broth

LB agar

S liquid medium

T-top (0.5% agar)

10 X M9 salts

10 X M9 salts + glucose

M9 agar

Supplemented M9 agar (M9sup)

Supplemented M9 broth (M9sup)

Vogel-Bonner glucose (VBG) minimal medium

50X Vogel-Bonner salts

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