User:Andy Maloney/Surface passivation study
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Introduction
Below you will find the notebook entries that I believe are the most relevant ones for the surface passivation paper. Some entries show the days I was taking data, some show my preparations for data taking, and others show spectacular failures that occurred in the lab that I had to fix in order to run my experiments. I am hopeful that this information will be useful to at least one person on the planet now, or in the future.
Notebook entries
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/24/Objective temperature control
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Mapping out what I should do about stabilizing the temperature of the objective. Also, this page describes my initial study on temperature affects.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/25/More on kinesin speed effects due to temperature
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: his is a follow up to the initial study of temperature issues.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/27/Casein data revisited
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules, Surface passivation
- Description: Reanalyzing the casein data I took to incorporate the times I took the data at. This shows a temperature affect going on.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/07/LED for microscope temperature control
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: This is my first look at how the Thorlabs LED controller works. The goal is to replace the existing LED with another LED so that I can get a broad spectrum of colors in the microscope.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/17/Temperature stabilization
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Objective temperature stabilization build, slide warmer, and LED discussions.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/23/Temperature data
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Posting data with temperature stabilization as well as looking more at the LED from Bridgelux. Also, an experiment where I look at only one spot in the sample.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/25/LED and Spot test
- Categories: AM Surface passivation paper, Kinesin and microtubules
- Description: Data from the other day where I looked at GMA with an LED and continued illumination with the Hg lamp.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/29/Whole casein with temperature stabilization
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: I now have repeated experiments where I have stabilized the temperature of the objective and use whole casein as the passivation.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/19
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Beginning to notice that my casein solutions are no longer working. All I changed was the temperature stabilization.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/20
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules
- Description: Making new solutions.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/26/Whole casein failure
- Categories: AM Engineering, AM SurfacePassivationMTs&Kinesin, AM Surface passivation paper, Kinesin and microtubule basics, Kinesin and microtubules
- Description: Making W-PEM and the failures that I encountered making it.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/26/Microscope repair
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Optical tweezers
- Description: Repairing an IX71 microscope.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/27/Reboot
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Initial test to see if all my new solutions work. No data.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/28/Caseins
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Looking at casein passivation with all the new solutions made. Beta, alpha, whole so far. Not looking so good.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/29/Beta casein test
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Trying the beta casein assay without putting it in the freezer and without filtering it and not putting it in the freezer. Guess what, it was the filtering.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/03/Temperature probe
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules
- Description: Getting the temperature probe working for the temperature stabilized objective.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/04/Solutions
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Again with the remaking of buffers and solutions.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/05/Solutions
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Finished with remaking solutions.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/05/Casein experiments
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Noting that the temperature I have the objective at is not hot enough to have a stable speed measurement.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/06/Temperature stabilization
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules
- Description: Epoxied the second thermistor to the objective since the oil used kept seeping under the tape.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/06/Casein studies
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: More casein studies with temperature stabilization. The beta casein assay is still acting "funny", or so I think.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/07/Passivation study
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: More surface passivation studies. I've figured out that the harder it is for a casein constituent to go into solution, the better it acts as a surface passivator. Plus, whole and alpha casein work great. Beta and kappa, not so much. Whole and alpha have motility at the center of the flow cell, beta and kappa has it only near the tape.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/10/Casein study
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Continued experiments using the different caseins as surface passivators.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/11/More caseins
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Taking the last bits of data for the bovine casein comparison experiment.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/12/Casein study
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Continuing the data taking for my passivation study. I've noticed that kappa and beta casein don't work very well when incubated at room temp. So, I'm going to try the cold thing again.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/13/More casein data taking
- Categories: AM Engineering, AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Getting the last data points for the passivation study. Also, dealing with the virtualbox update. And, making a stage micrometer.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/09/07/Casein data
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Looking at the movies I've made for the passivation study. I'm going to try an catalog everything I see in them.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/23
- Categories: AM Surface passivation paper, Kinesin and microtubules, Surface passivation
- Description: Today I tagged all my notebook entries that pertain to the surface passivation paper.
