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Knight Lab
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31 May 2023
| N 14:39 | UA Biophysics:Protocols:Carotenoid Extration ESP diffhist +423 Elizabeth Suesca talk contribs (Created page with "==Materiales para 6 gramos de célula== * Preparar centrífuga a 4 grados * 200 ml Metanol. Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * 500 NaCl 1,7 M (99.348 g/1 L) * 200 Acetato de etilo (Mejor si esta destilado ya que tiende a ser muy hidrófilo) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Cloroformo") | ||||
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14:34 | UA Biophysics:Protocols:Carotenoid Extration 2 changes history +86 [Elizabeth Suesca (2×)] | |||
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14:34 (cur | prev) −2 Elizabeth Suesca talk contribs | ||||
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14:34 (cur | prev) +88 Elizabeth Suesca talk contribs | ||||
| 14:24 | UA Biophysics:Protocols:K2HPO4 KH2PO4 Buffer diffhist +54 Elizabeth Suesca talk contribs | ||||
| 14:23 | UA Biophysics:Protocols diffhist −7 Elizabeth Suesca talk contribs (→BUFFERS) | ||||
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N 14:22 | UA Biophysics:Protocols:Elution Buffer 2 changes history +1,055 [Elizabeth Suesca (2×)] | |||
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14:22 (cur | prev) +467 Elizabeth Suesca talk contribs | ||||
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14:07 (cur | prev) +588 Elizabeth Suesca talk contribs (Created page with " '''Buffer HEPES: 2 L a 400 mOsM pH 7.4 ==Materiales== MW (g/mol) [] mM mOsM M HEPES 238.30 20 20 9.532 g NaCl 58.44 170 340 19.869 g NaOH (1 M) 8 8 16 ml NaCl 16 32 1.870 g Colocar 1.6 litros de agua deionizada en la botella Pesar el HEPES y el NaCl, diluir en los 1,6 L de dH2O Ajustar pH usando NaOH a 7.4. Calcular la osmolaridad y completar los 400 mOsm con NaCl Completar los dos litros UA Biop...") | |||
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N 14:03 | UA Biophysics:Protocols:Calcein ESP 3 changes history +1,249 [Elizabeth Suesca (3×)] | |||
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14:03 (cur | prev) +43 Elizabeth Suesca talk contribs (→Procedimiento:) | ||||
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14:02 (cur | prev) +63 Elizabeth Suesca talk contribs | ||||
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14:00 (cur | prev) +1,143 Elizabeth Suesca talk contribs (Created page with "'''Calceina: 400 mOsM, 50 mM, 100 ml, pH 7.4 ''' ==Materiales: == * Probeta 100 ml * 30 ml de NaOH (1M) * HCl (1M) * 3,113 g de calceina * 40 ml Buffer H: ** 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) ** HEPES 238.3 mg (para que quede a 10 mM en la solución final). ==Procedimiento: == # En la probeta medir 20 ml de NaOH 1M y colocar agitador # Disolver en el NaOH 3,113 g de calceina # Agregar los 40 ml del Buffer H a la calceina # A...") | |||
| 12:48 | BioMicroCenter:QC diffhist −421 Stuart S. Levine talk contribs (→AGILENT BIOANALYZER 2100) | ||||
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08:34 | UA Biophysics:Protocols:Calcein 2 changes history −751 [Elizabeth Suesca (2×)] | |||
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08:34 (cur | prev) +53 Elizabeth Suesca talk contribs | ||||
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08:33 (cur | prev) −804 Elizabeth Suesca talk contribs (Replaced content with " Calcein_ESP<br>") Tag: Replaced | ||||
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08:32 | UA Biophysics:Protocols:Bacteria for Lipid Extraction 4 changes history −1,049 [Elizabeth Suesca (4×)] | |||
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08:32 (cur | prev) +3 Elizabeth Suesca talk contribs | ||||
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08:31 (cur | prev) −1,205 Elizabeth Suesca talk contribs | ||||
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08:30 (cur | prev) +47 Elizabeth Suesca talk contribs | ||||
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08:29 (cur | prev) +106 Elizabeth Suesca talk contribs | ||||
| N 08:31 | UA Biophysics:Protocols:Bacteria for Lipid Extraction ESP diffhist +1,206 Elizabeth Suesca talk contribs (Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo...") | ||||
| 06:42 | UA Biophysics:Protocols:Lipid Extraction diffhist −9 Elizabeth Suesca talk contribs | ||||
30 May 2023
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23:52 | Hexlab 2 changes history +900 [Ximiao He (2×)] | |||
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23:52 (cur | prev) +440 Ximiao He talk contribs | ||||
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18:46 (cur | prev) +460 Ximiao He talk contribs | ||||
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20:13 | Holcombe:EyeTrackers 3 changes history −109 [Alex O. Holcombe (3×)] | |||
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20:13 (cur | prev) −1 Alex O. Holcombe talk contribs (→Eyelink 1000 (scattered around the department)) | ||||
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20:12 (cur | prev) +130 Alex O. Holcombe talk contribs (→Eyelink 1000 (scattered around the department)) | ||||
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20:11 (cur | prev) −238 Alex O. Holcombe talk contribs (→Eyelink 1000 (scattered around the department)) | ||||
| 16:25 | Lee:JC diffhist +7 Wooin Lee talk contribs (→Schedule) | ||||
| 14:31 | Tomlinson:LabMeeting diffhist −726 Ryan E. Tomlinson talk contribs | ||||
| 06:44 | UA Biophysics:Protocols:Lipid Extraction diffhist +89 Elizabeth Suesca talk contribs | ||||
29 May 2023
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15:53 | CHEM-ENG590E:Wiki Textbook 9 changes history −311 [Sarah L. Perry (9×)] | |||
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15:53 (cur | prev) −59 Sarah L. Perry talk contribs (→Chapter 3 - 3D Printing) | ||||
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15:48 (cur | prev) −53 Sarah L. Perry talk contribs (→Chapter 3 - 3D Printing) | ||||
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15:48 (cur | prev) −91 Sarah L. Perry talk contribs (→Chapter 3 - 3D Printing) | ||||
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15:47 (cur | prev) −26 Sarah L. Perry talk contribs (→Chapter 3 - 3D Printing) | ||||
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15:46 (cur | prev) +27 Sarah L. Perry talk contribs (→Chapter 3 - 3D Printing) | ||||
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15:45 (cur | prev) +59 Sarah L. Perry talk contribs (→Chapter 3 - 3D Printing) | ||||
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15:42 (cur | prev) −101 Sarah L. Perry talk contribs (→Chapter 3 - 3D Printing) | ||||
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15:40 (cur | prev) +4 Sarah L. Perry talk contribs (→Chapter 3 - 3D Printing) | ||||
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15:26 (cur | prev) −71 Sarah L. Perry talk contribs (→Chapter 3 - 3D Printing) | ||||
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15:53 | Applications of 3D Printing 2 changes history −187 [Sarah L. Perry (2×)] | |||
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15:53 (cur | prev) −15 Sarah L. Perry talk contribs (→Soft Robotics) | ||||
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15:44 (cur | prev) −172 Sarah L. Perry talk contribs | ||||
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15:37 | Common 3D Printing Materials 10 changes history +1,516 [Sarah L. Perry (10×)] | |||
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15:37 (cur | prev) 0 Sarah L. Perry talk contribs (→Bioprinting) | ||||
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15:37 (cur | prev) +9 Sarah L. Perry talk contribs (→Bioprinting) | ||||
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15:36 (cur | prev) +1,735 Sarah L. Perry talk contribs | ||||
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15:25 (cur | prev) +11 Sarah L. Perry talk contribs (→Polydimethylsiloxane (PDMS)) | ||||
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15:24 (cur | prev) +11 Sarah L. Perry talk contribs (→Acrylonitrile Butadiene Styrene (ABS)) | ||||
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15:22 (cur | prev) +1 Sarah L. Perry talk contribs (→Acrylonitrile Butadiene Styrene (ABS)) | ||||
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15:21 (cur | prev) −12 Sarah L. Perry talk contribs (→Acrylonitrile Butadiene Styrene (ABS)) | ||||
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15:20 (cur | prev) −223 Sarah L. Perry talk contribs (→Polydimethylsiloxane (PDMS)) | ||||
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15:17 (cur | prev) −10 Sarah L. Perry talk contribs (→Polylactic Acid (PLA)) | ||||
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15:17 (cur | prev) −6 Sarah L. Perry talk contribs (→Polylactic Acid (PLA)) | ||||
| 15:25 | BioMicroCenter:HT Tech diffhist +57 Stuart S. Levine talk contribs | ||||
| 15:22 | BioMicroCenter:Sequencing diffhist −1 Stuart S. Levine talk contribs (→SHORT READ SEQUENCING) | ||||
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