Knight
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Knight Lab
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About Us
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Technical Info |
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Recent changes
List of abbreviations:
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3 June 2023
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23:44 | Lee:JC 6 changes history +26 [Wooin Lee (6×)] | |||
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23:44 (cur | prev) +9 Wooin Lee talk contribs (→Schedule) | ||||
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23:23 (cur | prev) −10 Wooin Lee talk contribs (→Schedule) | ||||
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23:22 (cur | prev) +6 Wooin Lee talk contribs (→Schedule) | ||||
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23:21 (cur | prev) +7 Wooin Lee talk contribs (→Schedule) | ||||
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23:01 (cur | prev) +7 Wooin Lee talk contribs (→Schedule) | ||||
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22:53 (cur | prev) +7 Wooin Lee talk contribs (→Schedule) | ||||
1 June 2023
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23:23 | Lee:Lab Members 4 changes history +169 [Wooin Lee (4×)] | |||
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23:23 (cur | prev) +164 Wooin Lee talk contribs (→Research rotation/internship students (past)) | ||||
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23:21 (cur | prev) +26 Wooin Lee talk contribs (→Graduate students) | ||||
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23:20 (cur | prev) +31 Wooin Lee talk contribs (→Alumni) | ||||
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23:19 (cur | prev) −52 Wooin Lee talk contribs (→Graduate students) | ||||
| 17:46 | BioMicroCenter:Pricing diffhist −79 Stuart S. Levine talk contribs (→SHORT READ LIBRARY - RNA) | ||||
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09:06 | UA Biophysics:Protocols:Carotenoid Extration ESP 5 changes history +2,390 [Elizabeth Suesca (5×)] | |||
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09:06 (cur | prev) +53 Elizabeth Suesca talk contribs | ||||
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09:05 (cur | prev) +59 Elizabeth Suesca talk contribs | ||||
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09:04 (cur | prev) +474 Elizabeth Suesca talk contribs | ||||
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08:43 (cur | prev) +781 Elizabeth Suesca talk contribs (→Procedimiento) | ||||
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08:25 (cur | prev) +1,023 Elizabeth Suesca talk contribs (→Materiales para 6 gramos de célula) | ||||
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09:04 | (Upload log) [Elizabeth Suesca (2×)] | |||
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09:04 Elizabeth Suesca talk contribs uploaded File:Cloroformo.png | ||||
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08:25 Elizabeth Suesca talk contribs uploaded File:Acetato.png | ||||
31 May 2023
| N 14:39 | UA Biophysics:Protocols:Carotenoid Extration ESP diffhist +423 Elizabeth Suesca talk contribs (Created page with "==Materiales para 6 gramos de célula== * Preparar centrífuga a 4 grados * 200 ml Metanol. Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * 500 NaCl 1,7 M (99.348 g/1 L) * 200 Acetato de etilo (Mejor si esta destilado ya que tiende a ser muy hidrófilo) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Cloroformo") | ||||
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14:34 | UA Biophysics:Protocols:Carotenoid Extration 2 changes history +86 [Elizabeth Suesca (2×)] | |||
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14:34 (cur | prev) −2 Elizabeth Suesca talk contribs | ||||
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14:34 (cur | prev) +88 Elizabeth Suesca talk contribs | ||||
| 14:24 | UA Biophysics:Protocols:K2HPO4 KH2PO4 Buffer diffhist +54 Elizabeth Suesca talk contribs | ||||
| 14:23 | UA Biophysics:Protocols diffhist −7 Elizabeth Suesca talk contribs (→BUFFERS) | ||||
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N 14:22 | UA Biophysics:Protocols:Elution Buffer 2 changes history +1,055 [Elizabeth Suesca (2×)] | |||
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14:22 (cur | prev) +467 Elizabeth Suesca talk contribs | ||||
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14:07 (cur | prev) +588 Elizabeth Suesca talk contribs (Created page with " '''Buffer HEPES: 2 L a 400 mOsM pH 7.4 ==Materiales== MW (g/mol) [] mM mOsM M HEPES 238.30 20 20 9.532 g NaCl 58.44 170 340 19.869 g NaOH (1 M) 8 8 16 ml NaCl 16 32 1.870 g Colocar 1.6 litros de agua deionizada en la botella Pesar el HEPES y el NaCl, diluir en los 1,6 L de dH2O Ajustar pH usando NaOH a 7.4. Calcular la osmolaridad y completar los 400 mOsm con NaCl Completar los dos litros UA Biop...") | |||
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N 14:03 | UA Biophysics:Protocols:Calcein ESP 3 changes history +1,249 [Elizabeth Suesca (3×)] | |||
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14:03 (cur | prev) +43 Elizabeth Suesca talk contribs (→Procedimiento:) | ||||
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14:02 (cur | prev) +63 Elizabeth Suesca talk contribs | ||||
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14:00 (cur | prev) +1,143 Elizabeth Suesca talk contribs (Created page with "'''Calceina: 400 mOsM, 50 mM, 100 ml, pH 7.4 ''' ==Materiales: == * Probeta 100 ml * 30 ml de NaOH (1M) * HCl (1M) * 3,113 g de calceina * 40 ml Buffer H: ** 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) ** HEPES 238.3 mg (para que quede a 10 mM en la solución final). ==Procedimiento: == # En la probeta medir 20 ml de NaOH 1M y colocar agitador # Disolver en el NaOH 3,113 g de calceina # Agregar los 40 ml del Buffer H a la calceina # A...") | |||
| 12:48 | BioMicroCenter:QC diffhist −421 Stuart S. Levine talk contribs (→AGILENT BIOANALYZER 2100) | ||||
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08:34 | UA Biophysics:Protocols:Calcein 2 changes history −751 [Elizabeth Suesca (2×)] | |||
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08:34 (cur | prev) +53 Elizabeth Suesca talk contribs | ||||
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08:33 (cur | prev) −804 Elizabeth Suesca talk contribs (Replaced content with " Calcein_ESP<br>") Tag: Replaced | ||||
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08:32 | UA Biophysics:Protocols:Bacteria for Lipid Extraction 4 changes history −1,049 [Elizabeth Suesca (4×)] | |||
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08:32 (cur | prev) +3 Elizabeth Suesca talk contribs | ||||
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08:31 (cur | prev) −1,205 Elizabeth Suesca talk contribs | ||||
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08:30 (cur | prev) +47 Elizabeth Suesca talk contribs | ||||
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08:29 (cur | prev) +106 Elizabeth Suesca talk contribs | ||||
| N 08:31 | UA Biophysics:Protocols:Bacteria for Lipid Extraction ESP diffhist +1,206 Elizabeth Suesca talk contribs (Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo...") | ||||
| 06:42 | UA Biophysics:Protocols:Lipid Extraction diffhist −9 Elizabeth Suesca talk contribs | ||||
30 May 2023
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23:52 | Hexlab 2 changes history +900 [Ximiao He (2×)] | |||
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23:52 (cur | prev) +440 Ximiao He talk contribs | ||||
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18:46 (cur | prev) +460 Ximiao He talk contribs | ||||
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20:13 | Holcombe:EyeTrackers 3 changes history −109 [Alex O. Holcombe (3×)] | |||
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20:13 (cur | prev) −1 Alex O. Holcombe talk contribs (→Eyelink 1000 (scattered around the department)) | ||||
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20:12 (cur | prev) +130 Alex O. Holcombe talk contribs (→Eyelink 1000 (scattered around the department)) | ||||
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20:11 (cur | prev) −238 Alex O. Holcombe talk contribs (→Eyelink 1000 (scattered around the department)) | ||||
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