When you create a new box, label it on all four sides so that the label can be seen regardless of which orientation the box is put back in the freezer.
Large -20°C freezer
Box Numbering: numeric shelf (top to bottom), alpha rack (left to right), numeric drawer (top to bottom), alpha box (front to back)
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|
Rack a
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Rack b
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Rack c
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Rack d
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| Shelf 1
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iGEM, UROPs and Julie
1a1a
Restriction enzymes
|
1a1b
MIT iGEM
|
1a1c
iGEM primers
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| 1a2a
|
1a2b
|
1a2c
iGEM/Randy primers?
|
1a3a
Julie
|
1a3b
Julie
|
1a3c
Julie's -20°C box
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1a4a
Julie's -20°C box 2
|
1a4b
Julie KAN
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1a4c
Julie's oligos and other stuff
|
|
|
|
Meagan and Austin
| 1d1a
|
1d1b Meagan's -20 box
|
1d1c
|
1d2a
Lysozyme
|
1d2b
Buffers
|
1d2c
|
1d3a
Pyrrolo-dC oligos
|
1d3b
Transcription
|
1d3c
Pyrophosphate assay
|
1d4a
Austin's Box
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1d4b
reserved for Austin
|
1d4c
Non-useful primers
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|
| Shelf 2
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Reshma
2a1a
synthetic transcription factors
|
2a1b
STF: device outputs
|
2a1c
STF: oligos
|
2a2a
working
|
2a2b
crosstalk assemblies
|
2a2c
crosstalk parts and subassemblies
|
2a3a
STF: device outputs with readout
|
2a3b
plasmid engineering
|
2a3c
plasmid engineering oligos
|
2a4a
STF subassemblies
|
2a4b
Device inputs
|
2a4c
Device low state
|
|
Tom
2b1a
Three antibiotic plasmid PCR
|
2b1b
Mesoplasma florum Transposases
|
2b1c
|
2b2a
Misc box
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2b2b
|
2b2c
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| 2b3a
|
2b3b
|
2b3c
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| 2b4a
|
2b4b
|
2b4c
|
|
Common lab reagents
2c1a
Common primers
|
2c1b
Common DNA
|
2c1c
|
2c2a
Restriction Enzymes 1 (A-B)
|
2c2b
Restriction Enzymes 2 (C-N)
|
2c2c
Restriction Enzymes 3 (O-Z)
|
2c3a
Polymerases
|
2c3b
BioBricks enzymes and buffers
|
2c3c
DNA and Protein ladders
|
2c4a
Ligases, phosphatases and kinases
|
2c4b
Antibiotic medium prep stocks
|
2c4c
RNAses and DNAses
|
|
Austin
2d1a
Ribozyme Constructs
|
2d1b
Ribozyme
|
2d1c
Ribozyme Primers
|
2d2a
BioBricks++ Plasmids
|
2d2b
BioBricks++ Parts
|
2d2c
BioBricks++ Primers
|
2d3a
BioBricks Plasmids
|
2d3b
BioBricks Parts
|
2d3c
Austin's Primers
|
2d4a
Light-control
|
2d4b
Promoters
|
2d4c
Empty
|
|
| Shelf 3
|
Old stuff
| 3a1a
|
3a1b
|
3a1c
Dig/Biotin labelled dNTPS, markers
|
3a2a
Randy's box
|
3a2b
Randy BioBricks
|
3a2c
|
3a3a
lacZ gene synthesis
|
3a3b
cI and derivatives
|
3a3c
arcT and unc 3030 dig probes
|
3a4a
|
3a4b
|
3a4c
|
|
|
|
Misc
3d1a
NEBuffers 1,2,3,4, BSA, MgSO4
|
3d1b
|
3d1c
|
3d2a
|
3d2b
|
3d2c
|
3d3a
|
3d3b
|
3d3c
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| 3d4a
|
3d4b
|
3d4c
|
|
| Shelf 4
|
Coolers
|
Coolers
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|
|
Reagents stored in boxes
- Amphoteracin B solution (Sigma)
- Antibiotic Antimycotic solution (Sigma)
- ATP (Amersham) 100 mM solution
- ATP A7699 1g
- L-Arginininamide A3913
- BCIP fast tablets B5655
- BCIP tablets B6024
- Benchmark HIS tagged protein standards
- BSA A7030
- bis-(2-mercaptoethyl) sulfone [NucleixPlus]
- Catalase C9322
- Catalase C40
- dl-p-chlorophenylalanine Aldrich 130710
- dl-p-chlorophenylalanine C6506
- N6 2' o-dibutyrl adenosine 3':5' cyclic monophosphate D0627
- DIG Chem-link (Roche)
- DIG-11-dUTP (Roche)
- DNAse I RNAse free Roche
- DTT (NEB)
- DTT
- 5' fluorescein phosphoramidite (2x)
- Iodoacetic acid I9148
- IPTG (5g)
- a-Ketoglutaric acid K1128
- Lysozyme L6876
- 4-methylumbeliferyl B-D-glucopyranoside
- 4-methylumbeliferyl b-D-glucosaminide
- N-(beta-keto caproyl) L-homoserine lactone K3007
- N-acetyl glucosamine A3286
- b-NAD N7004
- Oleic acid O1630
- Oligo dT-cellulose O3131
- PABA A9878
- d-Panothenic Acid P2250
- Pellet Paint NF (Novagen)
- Phenol, acid Ambion
- Protease P6911
- Protease inhibitor cocktail P8465
- Proteinase K (shelton scientific)
- Pyridoxal P9130
- Pyridoxaminhe P9380
- Ribonuclease A R4875
- Ribonuclease T1 R1003
- Rifampicin
- RNA Vanadyl complexes R3380
- RNAse, DNAse free Boehringer-Mannheim 1119915
- RNAse A R4642
- Spermidine S2501
- Spermine S1141 (2x)
- Stratagene Perfect Match PCR enhancer
- Streptavidin S4762
- SYBR Gold stain
- SybrGreen I nucleic acid stain
- SybrGreen II RNA stain
- Tetracycline
- Transfer RNA R8759
