BioMicroCenter:Pricing

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HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

PRICING UPDATE 6/15/2020

HISEQ2000 SEQUENCING

HISEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
Single End 40nt $1,200 $1,320 $1,800 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1y.
Other read lengths 7/1/18: Custom only. Requires purchase of full flowcell.

NEXTSEQ500 SEQUENCING

NEXTSEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
91nt kit
(eg. 40PE or 75SE)
$2,270 $2,497 $3,405 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
166nt kit
(eg. 80PE or 150SE)
$3,460 $3,807 $5,190
316nt kit
(eg. 150PE or 300SE)
$5,300 $5,830 $7,950

MISEQ SEQUENCING

MISEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
70nt V2 kit
(eg. 30PE or 70SE)
$1,250 $1,375 $1,875 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
150nt V3 kits
(eg. 75PE)
$1,350 $1,485 $2,025
300nt V2 kit
(eg. 150PE)
$1,475 $1,623 $2,213
500nt V2 kit
(eg. 250PE)
$1,703 $1,874 $2,553
600nt V3 kit
(eg. 300PE)
$2,043 $2,248 $3,065
500nt NANO Run
(eg. 250PE)
$730 $803 $1,095

NOVASEQ SEQUENCING

NOVASEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
NovaSeq SP 100nt $3,533 $3,886 $5,300 Per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
NovaSeq SP 300nt $5,545 $6,100 $8,316
NovaSeq SP 500nt $7,613 $8,375 $11,420
NovaSeq S1 100nt $5,565 $6,121 $8,348
NovaSeq S1 200nt $7,245 $7,970 $10,868
NovaSeq S1 300nt $8,610 $9,471 $12,915
NovaSeq S2 100nt $11,445 $12,590 $17,168
NovaSeq S2 200nt $15,435 $16,800 $23,153
NovaSeq S2 300nt $18,060 $19,866 $27,090
NovaSeq S4 300nt Contact BioMicro NA

OTHER ILLUMINA SERVICES

ILLUMINA SUPPORTING SERVICES CORE LAB MIT Non-MIT unit Notes
Quality Control Only -only one QC is included per sequencing lane. $20 $26 $30 per sample Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. $10 $13 $15 per sample Includes Fluorometric quantification and Frag.Analyzer
qPCR only (Illumina) $15 $16.5 $22.5 per sample Includes qPCR values from standard QC only
qPCR only - full plate(Illumina) $300 $330 $450 per sample excludes column 1 (used for controls)

LONG READ SEQUENCING

SEQUEL SEQUENCING CORE LAB MIT Non-MIT unit Notes
10 hour v3 Chemistry $1,340 $1,475 $2,010 per SMRTCell Includes polymerase binding and movie.
20 hour v3 Chemistry $1,470 $1,617 $2,205
PROMETHION SEQUENCING CORE LAB MIT Non-MIT unit Notes
72 hour Flowcell $2,000 $2,200 $3,000 per Flowcell Includes 1-2 nuclease wash & reload.
Fastq stored for 1y. Trace data stored only 30d.

LIBRARY GENERATION

ILLUMINA LIBRARY - DNA

ILLUMINA Sample Preparation Type Batch Size CORE LAB MIT Non-MIT Notes
STANDARD THROUGHPUT DNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
HIGH THROUGHPUT DNA
Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
LIGATION BASED
NEB UltraII
STD per sample $105 $115 $157 Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
HTL 24 $790 $869 $1185 Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
96 $1575 $1732 $2362
Tagmentation
NEXTERA-XT
STD per Sample $105 $115 $157 Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
Tagmentation
NEXTERA-FLEX
$157 $173 $236
NEXTERA-XT OR FLEX HTL 24 $790 $869 $1185 Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
96 $1575 $1732 $2362
16S Amplicon HTL 48 $1,890 $2,080 $2,835 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
add'l 48 $630 $693 $945
Other Amplicon HTL 48 $1,512 $1,663 $2,268 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
Samples *must* be submitted amplified with appropriate linkers.
add'l 48 $630 $693 $945
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $50 $55 $75
Sample Cleanup per sample $5 $5.5 $7.5 SPRI cleanup of samples to remove impurities.
Quantification per sample $5 $5.5 $7.5 Quantification of samples (FA) prior to arraying.

ILLUMINA LIBRARY - RNA

ILLUMINA Sample Preparation Type Batch Size CORE LAB MIT Non-MIT Notes
STANDARD RNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
HIGH THROUGHPUT RNA
Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included
polyA based isolation

Kapa mRNA Hyperprep
OR
NEB UltraII RNAseq
STD per sample $157 $173 $236 Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
HTL
(NEB only)
24 $1,310 $1,444 $1,969 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $2,620 $2,888 $3,937 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
High Throughput 3' Digital Gene Expression (HT3DGE) HTL 24 $1050 $1155 $1575 Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
96 $2100 $2310 $3150
Total RNA sequencing:Mammalian

Kapa Hyperprep + RiboErase
OR
NEB RNAseq +Ribodepletion
STD per sample $235 $258.5 $352.5 Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
HTL
(NEB only)
24 $2100 $2310 $3150 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $4200 $4620 $6300
Low Input polyA+
Clontech SMARTseq + NexteraXT or Flex
STD
v4
per sample $262 $289 $394 Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
HTL
v2
24 $1680 $1848 $2520
96 $2625 $2888 $3938
Low Input Mammalian Total
Clontech ZapR Kit
STD per sample $210 $231 $315 Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
Small RNA STD per sample $289 $318 $433 Uses NEB, BIOO or QIAGEN small RNA kit
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $50 $55 $75
Sample Cleanup per sample $5 $5.5 $7.5 SPRI cleanup of samples to remove impurities.
Quantification per sample $5 $5.5 $7.5 Quantification of samples (FA) prior to arraying.

.

LONG READ LIBRARIES

PacBio Sample Preparation Batch Size CORE LAB MIT Non-MIT Notes
SMRTBell Only
eg:PCR products
Sample 1 $315 $346 $472 Includes QC, repair and addition of indexed SMRTBells
Samples 2-8 $157 $173 $236
Genomic Library Sample 1 $472 $520 $709 Includes Covaris gSPIN fragmentation, Pippin sizing, repair and SMRTbell as above
Sample 2-8 $235 $258 $352
Oxford Nanopore Sample Preparation Nanopore Kit CORE LAB MIT Non-MIT Notes
Standard Ligation LSK109 $150 $165 $225 Oxford Nanopore prep methods remain highly experimental and are continually in flux.
As such, we can only estimate cost based on reagents/effort and cannot guarantee results.
Please consult the core prior to sample submission for the latest information.
SMARTseq -> Ligation Cv5+LSK109 $315 $346 $472
Rapid Prep (tagment - ~10-20kb) RAD004 $157 $173 $236
Long Insert Prep RAD004 (modified, target >100kb) $262 $289 $394
Direct RNA RNA002 $210 $231 $315

SINGLE CELL SERVICES

SEQWELL

10X USAGE Batch Size KI OTHER CORE LAB MIT Non-MIT Notes
Assisted Service
FULL SERVICE
Begin with cells
per array - - - - Includes all steps, QC of cDNA
Begins in KI Nanowell Core
FROM cDNA on BEADS per sample $350 $350 $385 $525 Includes exonuclease treatment, amplification, QC of cDNA and library production
Must coordinate with BMC staff on submission.
FROM AMPLIFIED cDNA per sample $100 $100 $110 $150 Includes QC of cDNA and library production only.
Reagents for SeqWell
SeqWell Array contact shanu@mit.edu for pricing and availability
SeqWell beads

10X CHROMIUM

10X USAGE Batch Size CORE LAB MIT Non-MIT Notes
Assisted Service
DIGITAL GENE EXPRESSION
5'RNA
3' RNA
setup $866 $953 $1299 charge per submission
per sample $1400 $1540 $2100 Includes QC of cDNA and creation of Illumina Library
Amplicon (TCR, BCR, antibody barcocde // per amplicon) setup $367 $404 $551 additional prep beyond DGE
per sample $30 $33 $45 Includes QC of cDNA and creation of Illumina Library
scATACseq
ATACseq setup $750 $825 $1125 charge per submission
per sample $1350 $1485 $2025 Includes QC of cDNA and creation of Illumina Library
scCNV
CNV setup $1365 $1501 $2047 charge per submission
per sample $1300 $1430 $1950 Includes QC of cDNA and creation of Illumina Library
CANCELLATION
per session $150 $165 $225 <24h notice
Walk-up Service
3'/5'RNA Beads and Reagents (v3) per samples $1350 $1485 $2025
ATACseq Beads and Reagents* per sample $1280 $1408 $1920
scCNV Beads and Reagents* per sample $1330 $1463 $1995
Chips (up to 8 sample) per chip $350 $385 $525 Includes service contract charge.
Training
Limited to 2 people
per method & per session
Reagents not included
$550 $605 $825
  • please let BMC know if you plan to run this method early so we will have reagents ready for you.

NAMOCELL

NAMOCELL Batch Size CORE LAB MIT Non-MIT Notes
Usage Per run $20 $22 $30 per hour
Cartridge 1 cartridge per sample $60 $66 $90

OTHER GENOMICS SERVICES


QUALITY CONTROL

QUALITY CONTROL unit CORE LAB MIT Non-MIT Notes
Fragment Analyzer: RNA or DNA per sample $8 $9 $12
Fragment Analyzer: Preloaded row per row (12) $44 $48 $66 2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA per sample $12 $13 $18
FemtoPulse: Preloaded Row per row (12) $66 $73 $99 2ul per sample in each well using plates from BMC
BioAnalyzer: Assisted Service (RNA or DNA) per sample $15 $18 $25
BioAnalyzer: Assisted Service (High Sensitivity protein) per sample $40 $44 $60 detection of proteins at < 30ng/ul.
qPCR only (Illumina quantification test) per sample $20 $22 $30 4 point serial dilution
Full plate qPCR (Illumina quantification test) per plate $300 $330 $450 full plate only (exclude column 1 for standards) - must be to BMC specifications.

MICROARRAYS

MICROARRAYS unit CORE LAB MIT Non-MIT Notes
AGILENT SCANNER per slide $50 $50 N/A Price per glass slide.

Agilent scanning services will end 6/30/2019
.

REAL TIME PCR

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
Roche LightCycler 480 $10 N/A per plate CALENDAR *available for CORE only Contact Noelani Kamelamela for training
SYBR Green (KAPA/Roche) $36 N/A per ml Available for MIT only
96-well PLATES $6 N/A per plate Available for MIT only

.

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
COVARIS SONICATOR
COVARIS E220 $20 $30 per hour ilab calendar tubes available for purchase.
OLIGO SYNTHESIS
Assisted $1000
+consumables
$1500
+consumables
per run includes setup and run of the instrument only.
Walkup $500
+consumables
NA per run includes =run of the instrument only.
Training $2000 N/A includes two instrument runs.
PIPPIN PREP
Gel Isolation $50 $75 per gel (5 lanes) TBA
Training $25 $50 per session TBA Contact Noelani Kamelamela for training.
PLATE READER
Varioskan $18 $27 per hour CALENDAR Contact Noelani Kamelamela for training
ROBOTICS
Tecan EVO 150 $50 $55 per 1h block - based on scheduled time. CALENDAR
non-filter tips $6.00 $6.00 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $8.00 $8.00 per box
Training $50 $55 per session Contact Austin Hendricks
NANODROP
Nanodrop -- -- Walk up service * no charge



COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES unit CORE LAB Other MIT / non-MIT Notes
Informatics Support per hour $85 $115 / $160 Very limited support for non-MIT users
Informatics Share per 0.1FTE $1,150 N/A MIT only. Priority access to informatics.
Ingenuity Pathway Analysis per dataset $100 $110/$150 Recovery of licence cost.
Active Data storage per TB per year $200 $300 / N/A
Archival Storage (read only. Unmutable) per TB per year $100 $150 / N/A
Tape Archive / Recovery per tape $60 $70


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