BioMicroCenter:Pricing

From OpenWetWare
Jump to navigationJump to search

BMC Header 2020 3.png

HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

PRICING UPDATE 7/1/2022

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

NOVASEQ SEQUENCING

NOVASEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
NovaSeq: 400m reads $1,600 $1,760 $2,240 Per 400m reads (max 150PE) Requires BMC library prep, Max 96 samples
NovaSeq SP 100nt $3,425 $3,768 $4,795 Per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
NovaSeq SP 300nt** $4,850 $5,335 $6,790
NovaSeq SP 500nt $6,500 $7,150 $9,100
NovaSeq S1 100nt** $5,400 $5,940 $7,560
NovaSeq S1 200nt** $6,900 $7,590 $9,660
NovaSeq S1 300nt** $7,525 $8,8278 $10,535
NovaSeq S2 100nt** $9,625 $10,588 $13,475
NovaSeq S2 200nt** $12,000 $13,200 $16,800
NovaSeq S2 300nt** $12,500 $13,750 $17,500
NovaSeq S4 50nt $3,500 $3,850 $4,900 Per Lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
NovaSeq S4 300nt $5,000 $5,500 $7,000
    • - minimal inventory maintained

NEXTSEQ500 SEQUENCING

NEXTSEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
91nt kit
(eg. 40PE or 75SE)
$2,200 $2,420 $3,080 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
166nt kit
(eg. 80PE or 150SE)
$3,750 $4,125 $5,250
316nt kit
(eg. 150PE or 300SE)
$5,700 $6,270 $7,980

MISEQ SEQUENCING

MISEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
70nt V2 kit
(eg. 30PE or 70SE)
$1,200 $1,320 $1,680 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year
150nt V3 kits
(eg. 75PE)
$1,300 $1,430 $1,820
300nt V2 kit
(eg. 150PE)
$1,450 $1,595 $2,030
500nt V2 kit
(eg. 250PE)
$1,600 $1,760 $2,240
600nt V3 kit
(eg. 300PE)
$2,000 $2,200 $2,800
500nt NANO Run
(eg. 250PE)
$700 $770 $980

HISEQ2000 SEQUENCING

The HiSeq2000 reagents will no longer be available in 2023. Please convert experiments as rapidly as possible to other platforms.

HISEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
Single End 40nt $1,200 $1,320 $1,800 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1y.
Other read lengths 7/1/18: Custom only. Requires purchase of full flowcell.

OTHER ILLUMINA SERVICES

ILLUMINA SUPPORTING SERVICES CORE LAB MIT Non-MIT unit Notes
Quality Control Only -only one QC is included per sequencing lane. $20 $26 $30 per sample Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. $10 $13 $15 per sample Includes Fluorometric quantification and Frag.Analyzer
qPCR only (Illumina) $15 $16.5 $22.5 per sample Includes qPCR values from standard QC only
qPCR only - full plate(Illumina) $300 $330 $450 per sample excludes column 1 (used for controls)

LONG READ SEQUENCING

SEQUEL SEQUENCING CORE LAB MIT Non-MIT unit Notes0
20 hour v3 Chemistry $1,470 $1,617 $2,205 per SMRTCell Includes polymerase binding and movie.
PROMETHION SEQUENCING CORE LAB MIT Non-MIT unit Notes
72 hour Flowcell $950 $1,045 $1,330 per Flowcell Fastq stored for 1y. Trace data stored only 30d.

LIBRARY GENERATION

ILLUMINA LIBRARY - DNA

ILLUMINA Sample Preparation Type Batch Size CORE LAB MIT Non-MIT Notes
STANDARD THROUGHPUT DNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
HIGH THROUGHPUT DNA
Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
LIGATION BASED
NEB UltraII
STD per sample $105 $115 $157 Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
HTL 24 $790 $869 $1185 Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
96 $1575 $1732 $2362
Tagmentation
NEXTERA-XT
STD per Sample $105 $115 $157 Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
Tagmentation
NEXTERA-FLEX
$157 $173 $236
NEXTERA-XT OR FLEX HTL 24 $790 $869 $1185 Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA).
96 $1575 $1732 $2362
16S Amplicon HTL 48 $1,890 $2,080 $2,835 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
add'l 48 $630 $693 $945
Other Amplicon HTL 48 $1,512 $1,663 $2,268 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
Samples *must* be submitted amplified with appropriate linkers.
add'l 48 $630 $693 $945
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $50 $55 $75
Sample Cleanup per sample $5 $5.5 $7.5 SPRI cleanup of samples to remove impurities.
Quantification per sample $5 $5.5 $7.5 Quantification of samples (FA) prior to arraying.

ILLUMINA LIBRARY - RNA

ILLUMINA Sample Preparation Type Batch Size CORE LAB MIT Non-MIT Notes
STANDARD RNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
HIGH THROUGHPUT RNA
Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included
polyA based isolation

Kapa mRNA Hyperprep
OR
NEB UltraII RNAseq
STD per sample $157 $173 $236 Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
HTL
(NEB only)
24 $1,310 $1,444 $1,969 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $2,620 $2,888 $3,937 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
High Throughput 3' Digital Gene Expression (HT3DGE) HTL 24 $1050 $1155 $1575 Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
96 $2100 $2310 $3150
Total RNA sequencing:Mammalian

Kapa Hyperprep + RiboErase
OR
NEB RNAseq +Ribodepletion
STD per sample $235 $258.5 $352.5 Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
HTL
(NEB only, covaris RNA fragmentation)
24 $2200 $2420 $3080 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $4400 $4840 $6160
Low Input polyA+
Clontech SMARTseq + NexteraXT or Flex
STD
v4
per sample $262 $289 $394 Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
HTL
v2
24 $1680 $1848 $2520
96 $2625 $2888 $3938
Low Input Mammalian Total
Clontech ZapR Kit
STD per sample $210 $231 $315 Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
HTL
(covaris RNA fragmentation)
24 $2200 $2420 $3080 Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA).
96 $4400 $4840 $6160
Small RNA STD per sample $289 $318 $433 Uses NEB, BIOO or QIAGEN small RNA kit
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $50 $55 $75
Sample Cleanup per sample $5 $5.5 $7.5 SPRI cleanup of samples to remove impurities.
Quantification per sample $5 $5.5 $7.5 Quantification of samples (FA) prior to arraying.

.

LONG READ LIBRARIES

PacBio Sample Preparation Batch Size CORE LAB MIT Non-MIT Notes
SMRTBell Only
eg:PCR products
Sample 1 $315 $346 $472 Includes QC, repair and addition of indexed SMRTBells
Samples 2-8 $157 $173 $236
Genomic Library Sample 1 $472 $520 $709 Includes Covaris gSPIN fragmentation, Pippin sizing, repair and SMRTbell as above
Sample 2-8 $235 $258 $352
Oxford Nanopore Sample Preparation Nanopore Kit CORE LAB MIT Non-MIT Notes
Standard Ligation LSK $160 $176 $224 Oxford Nanopore prep methods remain highly experimental and are continually in flux.
As such, we can only estimate cost based on reagents/effort and cannot guarantee results.
Please consult the core prior to sample submission for the latest information.
Native Barcoding
(ligation indexing)
- $50 $55 $70
SMARTseq -> Ligation Cv4+LSK $300 $330 $420
Rapid Prep (tagment - ~10-20kb) RAD $150 $165 $210
Long Insert Prep RAD (modified, target >100kb) $262 $289 $394
Direct RNA RNA002 $160 $176 $225

SINGLE CELL & SPATIAL SERVICES

10X CHROMIUM

10X USAGE Batch Size CORE LAB MIT Non-MIT Notes
Assisted Service
DIGITAL GENE EXPRESSION
5'RNA
3' RNA
setup $825 $908 $1155 charge per submission
per sample $1537 $1690 $2,151 Includes QC of cDNA and creation of Illumina Library
Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon) setup $350 $385 $490 additional prep beyond DGE
per sample $40 $44 $56 Includes QC of cDNA and creation of Illumina Library
scATACseq
ATACseq setup $715 $787 $1000 charge per submission
per sample $1475 $1623 $2065 Includes QC and creation of Illumina Library
Multiome (ATAC+3'RNA)
MULTI setup $1485 $1633.5 $2079 charge per submission
per sample $2720 $2992 $3808 Includes QC of cDNA and creation of Illumina Library
CANCELLATION
per session $200 $220 $280 <24h notice
  • please let BMC know if you plan to run this method early so we will have reagents ready for you.

NAMOCELL

NAMOCELL Batch Size CORE LAB MIT Non-MIT Notes
Usage Per run $20 $22 $30 per hour
Cartridge 1 cartridge per sample $60 $66 $90

VISIUM

SLIDES Batch Size KI Only Other CORE LAB MIT Non-MIT Notes
Test Array slide $1157.75 $1501.5 $1510.5 $2216 recommended 1x per tissue type
Sample Slide 1 slide / 4 tiles $5480 $5614 $6200 $7672 Includes H&E stain images + Library Generation

OTHER GENOMICS SERVICES


QUALITY CONTROL

QUALITY CONTROL unit CORE LAB MIT Non-MIT Notes
Fragment Analyzer: RNA or DNA per sample $8 $9 $12
Fragment Analyzer: Preloaded row per row (12) $44 $48 $66 2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA per sample $12 $13 $18
FemtoPulse: Preloaded Row per row (12) $66 $73 $99 2ul per sample in each well using plates from BMC
BioAnalyzer: Assisted Service (RNA or DNA) per sample $15 $18 $25
BioAnalyzer: Assisted Service (High Sensitivity protein) per sample $40 $44 $60 detection of proteins at < 30ng/ul.
qPCR only (Illumina quantification test) per sample $20 $22 $30 4 point serial dilution
Full plate qPCR (Illumina quantification test) per plate $300 $330 $450 full plate only (exclude column 1 for standards) - must be to BMC specifications.

REAL TIME PCR

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
Roche LightCycler 480 $10 N/A per plate CALENDAR *available for CORE only Contact Christopher Hallee for training
SYBR Green (KAPA/Roche) $36 N/A per ml Available for MIT only
96-well PLATES $6 N/A per plate Available for MIT only

.

OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
COVARIS SONICATOR
COVARIS E220 $20 $30 per hour ilab calendar tubes available for purchase.
OLIGO SYNTHESIS
Assisted $1000
+consumables
$1500
+consumables
per run includes setup and run of the instrument only.
Walkup $500
+consumables
NA per run includes =run of the instrument only.
Training $2000 N/A includes two instrument runs.
PIPPIN PREP
Gel Isolation $50 $75 per gel (5 lanes) TBA
Training $25 $50 per session TBA Contact Noelani Kamelamela for training.
PLATE READER
Varioskan $18 $27 per hour CALENDAR Contact Noelani Kamelamela for training
ROBOTICS
Tecan EVO 150 $50 $55 per 1h block - based on scheduled time. CALENDAR
non-filter tips $6.00 $6.00 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $8.00 $8.00 per box
Training $50 $55 per session Contact Sam Mildrum
NANODROP
Nanodrop -- -- Walk up service * no charge



COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES unit CORE LAB Other MIT / non-MIT Notes
Informatics Support per hour $90 $125 / $180 Very limited support for non-MIT users
Informatics Share per 0.1FTE $1,250 N/A MIT only. Priority access to informatics.
Ingenuity Pathway Analysis per dataset $100 $110/$150 Recovery of licence cost.
Active Data storage per TB per year $100 $300 / N/A
Tape Archive / Recovery per tape $60 $70
Informatics Training Classes per session $50 $100


OLD
FY2023 Pricing
FY2022 Pricing
FY2021 Pricing
FY2019 Pricing
FY2017 Pricing
FY2016 Pricing
FY2015 Pricing
FY2014 Pricing
FY2013 Pricing
FY2012 Pricing
FY2011 Pricing