BioMicroCenter:Pricing

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HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

HISEQ2000 SEQUENCING

HISEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
Single End 40nt $1,200 $1,320 $1,800 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
Other read lengths 7/1/18: Custom only. Requires purchase of full flowcell.

NEXTSEQ500 SEQUENCING

NEXTSEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
91nt kit
(eg. 40PE or 75SE)
$2,100 $2,320 $3,150 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
166nt kit
(eg. 80PE or 150SE)
$3,200 $3,520 $4,800
316nt kit
(eg. 150PE or 300SE)
$4,900 $5,390 $7,350

MISEQ SEQUENCING

MISEQ SEQUENCING CORE LAB MIT Non-MIT unit Notes
70nt V2 kit
(eg. 30PE or 70SE)
$1,155 $1,270 $1,732 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
150nt V3 kits
(eg. 75PE)
$1,250 $1,375 $1,875
300nt V2 kit
(eg. 150PE)
$1,365 $1,500 $2,050
500nt V2 kit
(eg. 250PE)
$1,575 $1,732 $2,362
600nt V3 kit
(eg. 300PE)
$1,890 $2,080 $2,835
500nt NANO Run
(eg. 250PE)
$675 $742.50 $1,012.50

OTHER ILLUMINA SERVICES

ILLUMINA SUPPORTING SERVICES CORE LAB MIT Non-MIT unit Notes
Quality Control Only -only one QC is included per sequencing lane. $20 $26 $30 per sample Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. $10 $13 $15 per sample Includes Fluorometric quantification and Frag.Analyzer

PACBIO SEQUENCING

SEQUEL SEQUENCING CORE LAB MIT Non-MIT unit Notes
10 hour v3 Chemistry $1,275 $1,405 $1,915 per SMRTCell Includes polymerase binding and movie.
20 hour v3 Chemistry $1,400 $1,540 $2,100

LIBRARY GENERATION

ILLUMINA LIBRARY - DNA

ILLUMINA Sample Preparation Batch Size CORE LAB MIT Non-MIT Notes
STANDARD THROUGHPUT DNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
HIGH THROUGHPUT DNA
Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included.
Standard Illumina Library (LM-PCR)
NEB UltraII
per sample $100 $110 $150 Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
24 samples $750 $825 $1075 Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA).
96 samples $1500 $1650 $2250
Tagmentation (NEXTERA-XT) - 1ng input 1 Sample $100 $110 $150 Includes tagmentation, indexing, enrichment, and quality control (SYBR and FA).
16
(2 column)
TECAN
$1000 $1100 $1500
96
Mosquito
$1500 $1650 $2250
384
Mosquito
$3000 $3300 $4500
16S Amplicon 48 samples
(6 columns)
$1,800 $1,980 $2,700 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
Other Amplicon 48 samples
(6 columns)
$1,440 $1,584 $2,160 Includes normalization, enrichment, pooling and quality control (SYBR and FA).
Samples *must* be submitted amplified with appropriate linkers.
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $50 $55 $75
Sample Cleanup per sample $5 $5.5 $7.5 SPRI cleanup of samples to remove impurities.
Quantification per sample $5 $5.5 $7.5 Quantification of samples (FA) prior to arraying.

ILLUMINA LIBRARY - RNA

ILLUMINA Sample Preparation Batch Size CORE LAB MIT Non-MIT Notes
STANDARD RNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
HIGH THROUGHPUT RNA
Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included
Standard polyA based isolation
Kapa mRNA Hyperprep
per sample $150 $165 $225 Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
24
TECAN
$2000 $2200 $3000 Includes polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
High Throughput 3' Digital Gene Expression (HT3DGE) 24
TECAN
$1000 $1100 $1500 Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
96
TECAN
$4000 $4400 $6000
Total RNA sequencing:Mammalian
Kapa Hyperprep + RiboErase
per sample $225 $247.5 $337.5 Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
Total RNA sequencing:Other
Kapa mRNA Hyperprep + Illumina RiboZero
per sample $250 $275 $375
Low Input polyA+
Clontech SMARTseq v4 + NexteraXT
1 sample $250 $275 $375 Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
Low Input polyA+
SMARTseq v2 + NexteraXT
24
Mosquito
$1600 $1760 $2400
96
Mosquito
$2500 $2750 $3750
Low Input Mammalian Total
Clontech ZapR Kit
1 sample $200 $220 $300 Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA).
Tight Size Cut per pool $85 $94 $130 +/- 40bp using PippinPrep
Small RNA per sample $275 $303 $413 Uses NEB, BIOO or QIAGEN small RNA kit
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup 48 samples $50 $55 $75
Sample Cleanup per sample $5 $5.5 $7.5 SPRI cleanup of samples to remove impurities.
Quantification 12 samples $5 $5.5 $7.5 Quantification of samples (FA) prior to arraying.

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PACBIO LIBRARIES

PacBio Sample Preparation Batch Size CORE LAB MIT Non-MIT Notes
SMRTBell Only
eg:PCR products
Sample 1 $300 $330 $450 Includes QC, repair and addition of indexed SMRTBells
Samples 2-8 $150 $165 $225
Genomic Library Sample 1 $450 $495 $675 Includes Covaris gSPIN fragmentation, Pippin sizing, repair and SMRTbell as above
Sample 2-8 $225 $250 $340

OTHER GENOMICS SERVICES


QUALITY CONTROL

QUALITY CONTROL unit CORE LAB MIT Non-MIT Notes
Fragment Analyzer: RNA or DNA per sample $8 $9 $12
Fragment Analyzer: Preloaded row per row (12) $30 $33 $45 2ul per sample in each well using plates from BMC
FemtoPulse: RNA or DNA per sample $10 $11 $15
FemtoPulse: Preloaded Row per row (12) $50 $55 $75 2ul per sample in each well using plates from BMC
BioAnalyzer: Assisted Service (RNA or DNA) per sample $15 $18 $25
BioAnalyzer: Assisted Service (High Sensitivity protein) per sample $40 $44 $60 detection of proteins at < 30ng/ul.

MICROARRAYS

MICROARRAYS unit CORE LAB MIT Non-MIT Notes
AGILENT SCANNER per slide $50 $50 N/A Price per glass slide.


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REAL TIME PCR

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
Roche LightCycler 480 $10 N/A per plate CALENDAR *available for CORE only Contact Noelani Kamelamela for training
SYBR Green (KAPA/Roche) $36 N/A per ml Available for MIT only
96-well PLATES $6 N/A per plate Available for MIT only

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OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
PIPPIN PREP
Gel Isolation $50 $75 per gel (5 lanes) TBA
Training $25 $50 per session TBA Contact Noelani Kamelamela for training.
PLATE READER
Varioskan $18 $27 per hour CALENDAR Contact Noelani Kamelamela for training
ROBOTICS
Tecan EVO 150 $50 $55 per 1h block - based on scheduled time. CALENDAR
non-filter tips $6.00 $6.00 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $8.00 $8.00 per box
Training $50 $55 per session Contact Stuart
NANODROP
Nanodrop -- -- Walk up service * no charge



COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES unit CORE LAB Other MIT / non-MIT Notes
Informatics Support per hour $75 $100 / $130 Very limited support for non-MIT users
Informatics Share per 0.1FTE $1,000 N/A MIT only. Priority access to informatics.
Active Data storage per TB per year $200 $300 / N/A
Archival Storage (read only. Unmutable) per TB per year $100 $150 / N/A
Tape Archive / Recovery per tape $60 $70

FY2017 Pricing
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FY2011 Pricing