BioMicroCenter:Sequencing

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HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

SHORT READ SEQUENCING

ILLUMINA

The BioMicro Center provides broad support for Illumina sequencing. The Center has five high-throughput Illumina sequencers including one NovaSeq 6000, two NextSeq500s and two MiSeqs. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end.

Illumina Sequencing is performed on a lane-by-lane basis instead of for a specific number of reads. This enables us to support a very broad spectrum of Illumina Library types as well as handle custom sequencing applications.

SINGULAR GENOMICS

The BioMicro Center will begin providing support for the Singular G4 sequencer in spring of 2023. This short read sequencer uses the same sequencing-by-synthesis chemistry as Illumina with modest modifications. Critically, the G4 allows addressable lanes at very competitive pricing.

LONG READ SEQUENCING / PACBIO and OXFORD NANOPORE

The BioMicro Center has made significant investments in long read sequencers. The Center has access to two separate platforms: Pacific Biosciences Sequel and Oxford Nanopore PromethION P24.

Pacific BioSciences sequencing is performed on the Sequel I with v3 chemistry. PacBio requests can be made through iLabs.

Oxford Nanopore Sequencing is still a new technology in the core but we are developing expertise that may be useful to researchers on campus. The center houses the Oxford Nanopore PromethION P24. Contaminants known to cause issues on the Nanopores so far include: EDTA, ethanol, isopropanol, NaCl, guanidinium chloride, guanidinium isothiocyanate, and phenol. Nanopore requests can be made through iLabs.

Beyond the sequencers, the BioMicro Center has additional tools that are critical to long read sequencing. These include an AATI FemtoPulse and a Sage BluePippin.

SANGER SEQUENCING

The MIT BioMicro Center *does not* support Sanger sequencing. Many third party providers exist for this method. For those who prefer to utilize core facilities instead of commercial providers, Sanger sequencing is available through the DF/HCC DNA Resource at the Dana-Farber Cancer Institute