BioMicroCenter:Sequencing

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HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

SHORT READ SEQUENCING

The BioMicro Center provides broad support for Illumina sequencing. The Center has five high-throughput Illumina sequencers including one HiSeq2000, two NextSeq500s and two MiSeqs. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end.

Numerous quality control metrics are built in to our Illumina sequencing and analysis pipeline to ensure high quality and highly reproducible data. The Center also has significant infrastructure to assist and creating Illumina libraries and in optimizing loading conditions.


LONG READ SEQUENCING

The BioMicro Center is beginning to make significant investments in long read sequencers. The Center has access to two separate platforms: Pacific Biosciences sequencing and Oxford Nanopore Minion.

Pacific BioSciences sequencing is performed on the RSII at the Umass Medical School Deep Sequencing Core Facility. PacBio requests can be made through iLabs.

Oxford Nanopore sequencing is still a new technology in the core but we are developing expertise that may be useful to researchers on campus. ONT minion runs are done on a custom bases only. Please email biomicro@mit.edu if you are interested in using the Minion.

Beyond the sequencers, the BioMicro Center has additional tools that are critical to long read sequencing. These include an AATI FemtoPulse and a Sage BluePippin.


SANGER SEQUENCING

The MIT BioMicro Center *does not* support Sanger sequencing. Many third party providers exist for this method. For those who prefer to utilize core facilities instead of commercial providers, Sanger sequencing is available through the DF/HCC DNA Resource at the Dana-Farber Cancer Institute