BioMicroCenter:Sequencing

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HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

SHORT READ SEQUENCING / ILLUMINA

The BioMicro Center provides broad support for Illumina sequencing. The Center has five high-throughput Illumina sequencers including one HiSeq2000, two NextSeq500s and two MiSeqs. We support a wide variety of applications, such as ChIP-Seq, miRNA sequencing and RNA-seq. Each lane can potentially accommodate dozens of indexed samples (depending on sequence complexity and desired coverage). Read lengths vary, depending on users, between 20nt and 325nt per end.

Illumina Sequencing is performed on a lane-by-lane basis instead of for a specific number of reads. This enables us to support a very broad spectrum of Illumina Library types as well as handle custom sequencing applications.

LONG READ SEQUENCING / PACBIO and OXFORD NANOPORE

The BioMicro Center has made significant investments in long read sequencers. The Center has access to two separate platforms: Pacific Biosciences Sequel and Oxford Nanopore Minion.

Pacific BioSciences sequencing is performed on the Sequel with v3 chemistry. PacBio requests can be made through iLabs.

Oxford Nanopore sequencing is still a new technology in the core but we are developing expertise that may be useful to researchers on campus. ONT minion runs are done on a custom basis only. Please email biomicro@mit.edu if you are interested in using the Minion.

Beyond the sequencers, the BioMicro Center has additional tools that are critical to long read sequencing. These include an AATI FemtoPulse and a Sage BluePippin.

SANGER SEQUENCING

The MIT BioMicro Center *does not* support Sanger sequencing. Many third party providers exist for this method. For those who prefer to utilize core facilities instead of commercial providers, Sanger sequencing is available through the DF/HCC DNA Resource at the Dana-Farber Cancer Institute