BioMicroCenter:RTPCR

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HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

Traditional Real Time PCR

Light Cycler 480 II Real-time PCR Machines

Instrument LC480
TYPE WALKUP - MIT only
UNIT Per Plate
FORMAT 96w/394w
SIGNUP ilabs
NEW USERS New users should request training by emailing biomicro@mit.edu
DONATED BY Prof Laurie Boyer, Prof Leonard Guarente, Dept of Biology

The LightCycler 480 Real-Time PCR System is a fully integrated multiwell-plate based real-time PCR platform for highly accurate qualitative and quantitative detection of nucleic acids. The instrument handles both 96 and 384w plates, rapidly switching between blocks (protocol below). The LC480 can measure Ct and melt curves and can do protein melt curves as well. The instrument handles SYBR and Taqman probes. ROC standards are not required on the instrument, though it can detect them.

There is a charge per plate to cover the cost of the service contract.

96 well plates and SYBR Green can also be purchased from the BioMicro Center for use on the LightCycler480

Please Note: There are three LightCycler systems (Thing 1, Thing 2, and Thing 3) that have a sign up maximum of four hours per day during business hours (10AM to 5PM), all time slots are available the night before even if it exceeds the maximum.


File:Block Changing Protocol LightCycler480.txt

LC480.jpg