BioMicroCenter:Illumina Library Preparation

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HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY
Sample Preparation available in BioMicro

Generating high-quality data on the Illumina sequencing platform requires high-quality libraries. The BMC currently offers library preparation services for a variety of starting materials. Prior to sequencing, all samples must pass the BioMicro Center’s Sequencing Quality Control process, which verifies selection of inserts of a desired size and correct ligation of Illumina adapters.

Illumina Library Prep Services

Please follow the links in the table below for more information about our library preparation offerings.

DNA RNA
Standard DNA Methods include
  • LMPCR methods
    • NEB UltraII
  • Tagmentation Methods
    • Nextera XT
    • Nextera Flex
Standard RNA Methods include
  • Kapa Hyperprep
    polyA stranded method
  • Kapa RiboErase or Illumina RiboZero
    stranded rRNA depletion
  • SMARTer_Low-Input
    Low input polyA based
  • Clontech ZapR
    Low input rRNA depletion based.
  • smallRNA prep
High Throughput DNA Methods include
  • Amplicon / 16S
  • NexteraXT
  • low input NexteraXT
  • NEB UltraII
High Throughput RNA Methods include
  • High Throughput 3' Digital Gene Expression (HT3DGE)
  • SMARTseq v2

LONG READ LIBRARY PREP SERVICES

The following services are available through the BioMicro Center. Some of these services are still experimental.

  • Pacific Biosciences - SMRTbell
  • Pacific Biosciences - isoSeq
  • Pacific Biosciences - genomic libraries
  • Oxford Nanopore - DNA
  • Oxford Nanopore - Direct RNA


OTHER SERVICES

SIZE SELECTION

The BMC offers PippinPrep Size Selection post library construction if custom insert sizes are needed.

QUALITY CONTROL

All NGS libraries are quality controlled prior to use. The BioMicro Center offers two 'flavors' of quality control for Illumina libraries.

Standard quality control includes analysis on the Fragment Analyzer and quantification by four point qPCR quantification for each sample. This confirms both the sizing as well as the presence of Illumina adapters on the libraries.

Rapid Quality Control is less expensive and quicker. It includes analysis on the Fragment Analyzer and quantification by PicoGreen on the Varioskan for each sample. Final pools are quantified by qPCR before loading. While this method is significantly faster and less expensive, we cannot guarantee pool balance with it.