BioMicroCenter:Illumina Library Preparation
|HOME --||SEQUENCING --||LIBRARY PREP --||HIGH-THROUGHPUT --||COMPUTING --||OTHER TECHNOLOGY|
Generating high-quality data on the Illumina sequencing platform requires high-quality libraries. The BMC currently offers library preparation services for a variety of starting materials. Prior to sequencing, all samples must pass the BioMicro Center’s Sequencing Quality Control process, which verifies selection of inserts of a desired size and correct ligation of Illumina adapters.
Illumina Library Prep Services
Please follow the links in the table below for more information about our library preparation offerings.
|Standard DNA Methods include
||Standard RNA Methods include
|High Throughput DNA Methods include
||High Throughput RNA Methods include
== LONG READ LIBRARY PREP SERVICES == submit 1 ug if insert size of dsDNA <4 kb, 5 ug if >4 kb
The following services are available through the BioMicro Center. Some of these services are still experimental.
- Pacific Biosciences - SMRTbell
- Pacific Biosciences - isoSeq
- Pacific Biosciences - genomic libraries
- Oxford Nanopore - DNA
- Oxford Nanopore - Direct RNA
The BMC offers PippinPrep Size Selection post library construction if custom insert sizes are needed.
All NGS libraries are quality controlled prior to use. The BioMicro Center offers two 'flavors' of quality control for Illumina libraries.
Standard quality control includes analysis on the Fragment Analyzer and quantification by four point qPCR quantification for each sample. This confirms both the sizing as well as the presence of Illumina adapters on the libraries.
Rapid Quality Control is less expensive and quicker. It includes analysis on the Fragment Analyzer and quantification by PicoGreen on the Varioskan for each sample. Final pools are quantified by qPCR before loading. While this method is significantly faster and less expensive, we cannot guarantee pool balance with it.