BioMicroCenter:PricingFY2023b
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PRICING UPDATE 2/1/2023
SEQUENCING
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
NOVASEQ SEQUENCING
| NOVASEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| NovaSeq: 400m reads | $1,760 | $1,936 | $2,464 | Per 400m reads (max 150PE) | Requires BMC library prep, Max 96 samples |
| NovaSeq SP 100nt | $3,699 | $4,068 | $5,178 | Per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| NovaSeq SP 300nt** | $5,238 | $5,761 | $7,333.20 | ||
| NovaSeq SP 500nt | $7,020 | $7,722 | $9,828 | ||
| NovaSeq S1 100nt** | $5,832 | $6,415.20 | $8,164.80 | ||
| NovaSeq S1 200nt** | $7,452 | $8,197.20 | $10,432 | ||
| NovaSeq S1 300nt** | $8,127 | $8,939.70 | $11,377.80 | ||
| NovaSeq S2 100nt** | $10,395 | $11,434.50 | $14,553 | ||
| NovaSeq S2 200nt** | $12,960 | $14,256 | $18,144 | ||
| NovaSeq S2 300nt** | $13,500 | $14,850 | $18,900 | ||
| NovaSeq S4 50nt | $3,780 | $4,158 | $5,292 | Per Lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| NovaSeq S4 300nt | $5,400 | $5,940 | $7,560 |
- - minimal inventory maintained
NEXTSEQ500 SEQUENCING
| NEXTSEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 91nt kit (eg. 40PE or 75SE) |
$2,420 | $2,662 | $3,388 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 166nt kit (eg. 80PE or 150SE) |
$4,125 | $4,537.50 | $5,775 | ||
| 316nt kit (eg. 150PE or 300SE) |
$6,270 | $6,870 | $8,778 |
MISEQ SEQUENCING
| MISEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 70nt V2 kit (eg. 30PE or 70SE) |
$1,320 | $1,452 | $1,848 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 150nt V3 kits (eg. 75PE) |
$1,430 | $1,573 | $2,002 | ||
| 300nt V2 kit (eg. 150PE) |
$1,595 | $1,754.50 | $2,233 | ||
| 500nt V2 kit (eg. 250PE) |
$1,760 | $1,936 | $2,464 | ||
| 600nt V3 kit (eg. 300PE) |
$2,200 | $2,420 | $3,080 | ||
| 500nt NANO Run (eg. 250PE) |
$770 | $847 | $1,078 |
HISEQ2000 SEQUENCING
The HiSeq2000 reagents will no longer be available in 2023. Please convert experiments as rapidly as possible to other platforms.
| HISEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| Single End 40nt | $1,200 | $1,320 | $1,800 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1y. |
| Other read lengths | 7/1/18: Custom only. Requires purchase of full flowcell. | ||||
OTHER ILLUMINA SERVICES
| ILLUMINA SUPPORTING SERVICES | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| Quality Control Only -only one QC is included per sequencing lane. | $25 | $27.50 | $35 | per sample | Includes RT-PCR and Frag.Analyzer |
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. | $12 | $13.20 | $16.80 | per sample | Includes Fluorometric quantification and Frag.Analyzer |
| qPCR only (Illumina) | $18 | $19.80 | $25.20 | per sample | Includes qPCR values from standard QC only |
| qPCR only - full plate(Illumina) | $330 | $363 | $462 | per sample | excludes column 1 (used for controls) |
LONG READ SEQUENCING
| SEQUEL SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 20 hour v3 Chemistry | $1,470 | $1,617 | $2,205 | per SMRTCell | Includes polymerase binding and movie. |
| PROMETHION SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
| 72 hour Flowcell | $1,200 | $1,320 | $1,680 | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
LIBRARY GENERATION
ILLUMINA LIBRARY - DNA
| ILLUMINA Sample Preparation | Type | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. | |||||
| LIGATION BASED NEB UltraII |
STD | per sample | $105 | $115 | $157 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). |
| HTL | 24 | $825 | $907.50 | $1,155 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA). | |
| 96 | $1,625 | $1,787.50 | $2,275 | |||
| Tagmentation NEXTERA-XT |
STD | per Sample | $105 | $115 | $157 | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). |
| Tagmentation NEXTERA-FLEX |
$157 | $173 | $236 | |||
| NEXTERA-XT OR FLEX | HTL | 24 | $825 | $907.50 | $1,155 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA). |
| 96 | $1,625 | $1,787.50 | $2,275 | |||
| 16S Amplicon | HTL | 48 | $1,900 | $2,090 | $2,660 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). |
| add'l 48 | $650 | $715 | $910 | |||
| Other Amplicon | HTL | 48 | $1,600 | $1,760 | $2,240 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers. |
| add'l 48 | $630 | $693 | $945 | |||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||||
| High Throughput Setup | 48 samples | $50 | $55 | $75 | ||
| Sample Cleanup | per sample | $5 | $5.50 | $7 | SPRI cleanup of samples to remove impurities. | |
| Quantification | per sample | $6 | $6.60 | $8.40 | Quantification of samples (FA) prior to arraying. | |
ILLUMINA LIBRARY - RNA
| ILLUMINA Sample Preparation | Type | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included | |||||
| polyA based isolation Kapa mRNA Hyperprep OR NEB UltraII RNAseq |
STD | per sample | $157 | $173 | $236 | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). |
| HTL (NEB only) |
24 | $1,400 | $1,540 | $1,960 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $2,800 | $3,080 | $3,920 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | ||
| High Throughput 3' Digital Gene Expression (HT3DGE) | HTL | 24 | $1,050 | $1,155 | $1,470 | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. |
| 96 | $2,100 | $2,310 | $2,940 | |||
| Total RNA sequencing:Mammalian Kapa Hyperprep + RiboErase OR NEB RNAseq +Ribodepletion |
STD | per sample | $235 | $258.5 | $352.5 | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. |
| HTL (NEB only, covaris RNA fragmentation) |
24 | $2,400 | $2,640 | $3,360 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $4,800 | $5,280 | $6,720 | |||
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex |
STD v4 |
per sample | $262 | $289 | $394 | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. |
| HTL v2 |
24 | $1880 | $1980 | $2520 | ||
| 96 | $2800 | $3080 | $3920 | |||
| Low Input Mammalian Total Clontech ZapR Kit |
STD | per sample | $210 | $231 | $315 | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). |
| HTL (covaris RNA fragmentation) |
24 | $2200 | $2420 | $3080 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $4400 | $4840 | $6160 | |||
| Small RNA | STD | per sample | $289 | $318 | $433 | Uses NEB, BIOO or QIAGEN small RNA kit |
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||||
| High Throughput Setup | 48 samples | $50 | $55 | $75 | ||
| Sample Cleanup | per sample | $5 | $5.50 | $7.50 | SPRI cleanup of samples to remove impurities. | |
| Quantification | per sample | $6 | $6.60 | $8.40 | Quantification of samples (FA) prior to arraying. | |
.
LONG READ LIBRARIES
| PacBio Sample Preparation | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| SMRTBell Only eg:PCR products |
Sample 1 | $315 | $346 | $472 | Includes QC, repair and addition of indexed SMRTBells |
| Samples 2-8 | $157 | $173 | $236 | ||
| Genomic Library | Sample 1 | $472 | $520 | $709 | Includes Covaris gSPIN fragmentation, Pippin sizing, repair and SMRTbell as above |
| Sample 2-8 | $235 | $258 | $352 | ||
| Oxford Nanopore Sample Preparation | Nanopore Kit | CORE LAB | MIT | Non-MIT | Notes |
| Standard Ligation | LSK | $160 | $176 | $224 | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information. |
| Native Barcoding (ligation indexing) |
- | $50 | $55 | $70 | |
| SMARTseq -> Ligation | Cv4+LSK | $300 | $330 | $420 | |
| Rapid Prep (tagment - ~10-20kb) | RAD | $150 | $165 | $210 | |
| Long Insert Prep | RAD (modified, target >100kb) | $262 | $289 | $394 | |
| Direct RNA | RNA002 | $160 | $176 | $225 |
SINGLE CELL & SPATIAL SERVICES
10X CHROMIUM
| 10X USAGE | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Assisted Service | |||||
| DIGITAL GENE EXPRESSION | |||||
| 5'RNA 3' RNA |
setup | $1,015 | $1,116.50 | $1,421 | charge per submission |
| per sample | $1,660 | $1,826 | $2,324 | Includes QC of cDNA and creation of Illumina Library | |
| Amplicon (CITE-SEQ, TCR, BCR, antibody barcocde, etc // per amplicon) | setup | $470 | $517 | $658 | additional prep beyond DGE |
| per sample | $40 | $44 | $56 | Includes QC of cDNA and creation of Illumina Library | |
| scATACseq | |||||
| ATACseq | setup | $870 | $957 | $1,218 | charge per submission |
| per sample | $1,585 | $1,743.50 | $2,219 | Includes QC and creation of Illumina Library | |
| Multiome (ATAC+3'RNA) | |||||
| MULTI | setup | $1,870 | $2,057 | $2,618 | charge per submission |
| per sample | $2,930 | $3,223 | $4,102 | Includes QC of cDNA and creation of Illumina Library | |
| CANCELLATION | |||||
| per session | $200 | $220 | $280 | <24h notice | |
- please let BMC know if you plan to run this method early so we will have reagents ready for you.
NAMOCELL
| NAMOCELL | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Usage | Per run | $20 | $22 | $30 | per hour |
| Cartridge | 1 cartridge per sample | $60 | $66 | $90 |
VISIUM
| SLIDES | Batch Size | KI Only | Other CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| Test Array | slide | $1,157.75 | $1,501.50 | $1,510.50 | $2,216 | recommended 1x per tissue type |
| Sample Slide | 1 slide / 4 tiles | $5,440 | $5,614 | $5,984 | $7,616 | Includes H&E stain images + Library Generation |
OTHER GENOMICS SERVICES
QUALITY CONTROL
| QUALITY CONTROL | unit | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Fragment Analyzer: RNA or DNA | per sample | $10 | $11 | $14 | |
| Fragment Analyzer: Preloaded row | per row (12) | $50 | $55 | $70 | 2ul per sample in each well using plates from BMC |
| FemtoPulse: RNA or DNA | per sample | $14 | $15.4 | $19.6 | |
| FemtoPulse: Preloaded Row | per row (12) | $70 | $77 | $98 | 2ul per sample in each well using plates from BMC |
| BioAnalyzer: Assisted Service (RNA or DNA) | per sample | $15 | $18 | $25 | |
| BioAnalyzer: Assisted Service (High Sensitivity protein) | per sample | $40 | $44 | $60 | detection of proteins at < 30ng/ul. |
| qPCR only (Illumina quantification test) | per sample | $18 | $19.8 | $25.2 | 4 point serial dilution |
| Full plate qPCR (Illumina quantification test) | per plate | $330 | $363 | $462 | full plate only (exclude column 1 for standards) - must be to BMC specifications. |
REAL TIME PCR
| OTHER LAB SERVICES | CORE LAB / MIT | Non-MIT | unit | SIGN UP | Notes |
|---|---|---|---|---|---|
| Roche LightCycler 480 | $10 | N/A | per plate | CALENDAR *available for CORE only | Contact Christopher Hallee for training |
| SYBR Green (KAPA/Roche) | $36 | N/A | per ml | Available for MIT only | |
| 96-well PLATES | $6 | N/A | per plate | Available for MIT only |
.
OTHER LAB SERVICES
| OTHER LAB SERVICES | CORE LAB / MIT | Non-MIT | unit | SIGN UP | Notes |
|---|---|---|---|---|---|
| COVARIS SONICATOR | |||||
| COVARIS E220 | $20 | $30 | per hour | ilab calendar | tubes available for purchase. |
| OLIGO SYNTHESIS | |||||
| Assisted | $1000 +consumables |
$1500 +consumables |
per run | includes setup and run of the instrument only. | |
| Walkup | $500 +consumables |
NA | per run | includes =run of the instrument only. | |
| Training | $2000 | N/A | includes two instrument runs. | ||
| PIPPIN PREP | |||||
| Gel Isolation | $75 | $105 | per gel (5 lanes) | TBA | |
| Training | $25 | $50 | per session | TBA | Contact Noelani Kamelamela for training. |
| PLATE READER | |||||
| Varioskan | $18 | $27 | per hour | CALENDAR | Contact Noelani Kamelamela for training |
| ROBOTICS | |||||
| Tecan EVO 150 | $50 | $55 | per 1h block - based on scheduled time. | CALENDAR | |
| non-filter tips | $7.00 | $7.70 | per box | ** Please let us know if there is additional plasticware you would like us to stock. | |
| Filter tips | $10.00 | $11.00 | per box | ||
| Training | $65 | $71.5 | per hour | Contact Tatum Murdock | |
| NANODROP | |||||
| Nanodrop | -- | -- | Walk up service | * no charge | |
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES | unit | CORE LAB | Other MIT / non-MIT | Notes |
|---|---|---|---|---|
| Informatics Support | per hour | $90 | $125 / $180 | Very limited support for non-MIT users |
| Informatics Share | per 0.1FTE | $1,250 | N/A | MIT only. Priority access to informatics. |
| Ingenuity Pathway Analysis | per dataset | $100 | $110/$150 | Recovery of licence cost. |
| Active Data storage | per TB per year | $100 | $300 / N/A | |
| Tape Archive / Recovery | per tape | $60 | $70 | |
| Informatics Training Classes | per session | $50 | $100 |
