BioMicroCenter:PricingFY2019
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SEQUENCING
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
HISEQ2000 SEQUENCING
| HISEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| Single End 40nt | $1,200 | $1,320 | $1,800 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y. |
| Other read lengths | 7/1/18: Custom only. Requires purchase of full flowcell. | ||||
NEXTSEQ500 SEQUENCING
| NEXTSEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 91nt kit (eg. 40PE or 75SE) |
$2,100 | $2,320 | $3,150 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |
| 166nt kit (eg. 80PE or 150SE) |
$3,200 | $3,520 | $4,800 | ||
| 316nt kit (eg. 150PE or 300SE) |
$4,900 | $5,390 | $7,350 |
MISEQ SEQUENCING
| MISEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 70nt V2 kit (eg. 30PE or 70SE) |
$1,155 | $1,270 | $1,732 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year |
| 150nt V3 kits (eg. 75PE) |
$1,250 | $1,375 | $1,875 | ||
| 300nt V2 kit (eg. 150PE) |
$1,365 | $1,500 | $2,050 | ||
| 500nt V2 kit (eg. 250PE) |
$1,575 | $1,732 | $2,362 | ||
| 600nt V3 kit (eg. 300PE) |
$1,890 | $2,080 | $2,835 | ||
| 500nt NANO Run (eg. 250PE) |
$675 | $742.50 | $1,012.50 |
OTHER ILLUMINA SERVICES
| ILLUMINA SUPPORTING SERVICES | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| Quality Control Only -only one QC is included per sequencing lane. | $20 | $26 | $30 | per sample | Includes RT-PCR and Frag.Analyzer |
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. | $10 | $13 | $15 | per sample | Includes Fluorometric quantification and Frag.Analyzer |
PACBIO SEQUENCING
| SEQUEL SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 10 hour v3 Chemistry | $1,275 | $1,405 | $1,915 | per SMRTCell | Includes polymerase binding and movie. |
| 20 hour v3 Chemistry | $1,400 | $1,540 | $2,100 |
LIBRARY GENERATION
ILLUMINA LIBRARY - DNA
| ILLUMINA Sample Preparation | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. | ||||
| Standard Illumina Library (LM-PCR) NEB UltraII |
per sample | $100 | $110 | $150 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). |
| 24 samples | $750 | $825 | $1075 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA). | |
| 96 samples | $1500 | $1650 | $2250 | ||
| Tagmentation (NEXTERA-XT) - 1ng input | 1 Sample | $100 | $110 | $150 | Includes tagmentation, indexing, enrichment, and quality control (SYBR and FA). |
| 16 (2 column) TECAN |
$1000 | $1100 | $1500 | ||
| 96 Mosquito |
$1500 | $1650 | $2250 | ||
| 384 Mosquito |
$3000 | $3300 | $4500 | ||
| 16S Amplicon | 48 samples (6 columns) |
$1,800 | $1,980 | $2,700 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). |
| add'l 48 samples (6 columns) |
$600 | $660 | $900 | ||
| Other Amplicon | 48 samples (6 columns) |
$1,440 | $1,584 | $2,160 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers. |
| add'l 48 samples (6 columns) |
$600 | $660 | $900 | ||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | |||||
| High Throughput Setup | 48 samples | $50 | $55 | $75 | |
| Sample Cleanup | per sample | $5 | $5.5 | $7.5 | SPRI cleanup of samples to remove impurities. |
| Quantification | per sample | $5 | $5.5 | $7.5 | Quantification of samples (FA) prior to arraying. |
ILLUMINA LIBRARY - RNA
| ILLUMINA Sample Preparation | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included | ||||
| Standard polyA based isolation Kapa mRNA Hyperprep |
per sample | $150 | $165 | $225 | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). |
| 24 TECAN |
$2000 | $2200 | $3000 | Includes polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). | |
| High Throughput 3' Digital Gene Expression (HT3DGE) | 24 TECAN |
$1000 | $1100 | $1500 | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. |
| 96 TECAN |
$4000 | $4400 | $6000 | ||
| Total RNA sequencing:Mammalian Kapa Hyperprep + RiboErase |
per sample | $225 | $247.5 | $337.5 | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. |
| Total RNA sequencing:Other Kapa mRNA Hyperprep + Illumina RiboZero |
per sample | $250 | $275 | $375 | |
| Low Input polyA+ Clontech SMARTseq v4 + NexteraXT |
1 sample | $250 | $275 | $375 | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. |
| Low Input polyA+ SMARTseq v2 + NexteraXT |
24 Mosquito |
$1600 | $1760 | $2400 | |
| 96 Mosquito |
$2500 | $2750 | $3750 | ||
| Low Input Mammalian Total Clontech ZapR Kit |
1 sample | $200 | $220 | $300 | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). |
| Tight Size Cut | per pool | $85 | $94 | $130 | +/- 40bp using PippinPrep |
| Small RNA | per sample | $275 | $303 | $413 | Uses NEB, BIOO or QIAGEN small RNA kit |
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | |||||
| High Throughput Setup | 48 samples | $50 | $55 | $75 | |
| Sample Cleanup | per sample | $5 | $5.5 | $7.5 | SPRI cleanup of samples to remove impurities. |
| Quantification | 12 samples | $5 | $5.5 | $7.5 | Quantification of samples (FA) prior to arraying. |
.
PACBIO LIBRARIES
| PacBio Sample Preparation | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| SMRTBell Only eg:PCR products |
Sample 1 | $300 | $330 | $450 | Includes QC, repair and addition of indexed SMRTBells |
| Samples 2-8 | $150 | $165 | $225 | ||
| Genomic Library | Sample 1 | $450 | $495 | $675 | Includes Covaris gSPIN fragmentation, Pippin sizing, repair and SMRTbell as above |
| Sample 2-8 | $225 | $250 | $340 |
SINGLE CELL SERVICES
SEQWELL
| 10X USAGE | Batch Size | KI | OTHER CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| Assisted Service | ||||||
| FULL SERVICE Begin with cells |
per array | - | - | - | - | Includes all steps, QC of cDNA Begins in KI Nanowell Core |
| FROM cDNA on BEADS | per sample | $350 | $350 | $385 | $525 | Includes exonuclease treatment, amplification, QC of cDNA and library production Must coordinate with BMC staff on submission. |
| FROM AMPLIFIED cDNA | per sample | $100 | $100 | $110 | $150 | Includes QC of cDNA and library production only. |
| Reagents for SeqWell | ||||||
| SeqWell Array | contact shanu@mit.edu for pricing and availability | |||||
| SeqWell beads | ||||||
10X CHROMIUM
| 10X USAGE | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Assisted Service | |||||
| 3'RNA (v3) | per sample | $1800 | $1980 | $2700 | Includes QC of cDNA and creation of Illumina Library |
| Walk-up Service | |||||
| 3'RNA Beads and Reagents (v3) | per samples | $1350 | $1485 | $2025 | |
| ATACseq Beads and Reagents* | per sample | $1280 | $1408 | $1920 | |
| scCNV Beads and Reagents* | per 4 samples | $1330 | $1463 | $1995 | |
| Chips (up to 8 sample) | per chip | $350 | $385 | $525 | Includes service contract charge. |
| Training Limited to 2 people |
per method & per session Reagents not included |
$500 | $550 | $750 | |
- please let BMC know if you plan to run this method early so we will have reagents ready for you.
NAMOCELL
| NAMOCELL | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Cartridge | 1 cartridge per sample | $75 | $85 | $125 | Includes service contract charge |
OTHER GENOMICS SERVICES
QUALITY CONTROL
| QUALITY CONTROL | unit | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Fragment Analyzer: RNA or DNA | per sample | $8 | $9 | $12 | |
| Fragment Analyzer: Preloaded row | per row (12) | $30 | $33 | $45 | 2ul per sample in each well using plates from BMC |
| FemtoPulse: RNA or DNA | per sample | $10 | $11 | $15 | |
| FemtoPulse: Preloaded Row | per row (12) | $50 | $55 | $75 | 2ul per sample in each well using plates from BMC |
| BioAnalyzer: Assisted Service (RNA or DNA) | per sample | $15 | $18 | $25 | |
| BioAnalyzer: Assisted Service (High Sensitivity protein) | per sample | $40 | $44 | $60 | detection of proteins at < 30ng/ul. |
MICROARRAYS
| MICROARRAYS | unit | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| AGILENT SCANNER | per slide | $50 | $50 | N/A | Price per glass slide. |
Agilent scanning services will end 6/30/2019
.
REAL TIME PCR
| OTHER LAB SERVICES | CORE LAB / MIT | Non-MIT | unit | SIGN UP | Notes |
|---|---|---|---|---|---|
| Roche LightCycler 480 | $10 | N/A | per plate | CALENDAR *available for CORE only | Contact Noelani Kamelamela for training |
| SYBR Green (KAPA/Roche) | $25 | N/A | per ml | Available for MIT only | |
| 96-well PLATES | $6 | N/A | per plate | Available for MIT only |
.
OTHER LAB SERVICES
| OTHER LAB SERVICES | CORE LAB / MIT | Non-MIT | unit | SIGN UP | Notes |
|---|---|---|---|---|---|
| COVARIS SONICATOR | |||||
| COVARIS E220 | $20 | $30 | per hour | ilab calendar | tubes available for purchase. |
| PIPPIN PREP | |||||
| Gel Isolation | $50 | $75 | per gel (5 lanes) | TBA | |
| Training | $25 | $50 | per session | TBA | Contact Noelani Kamelamela for training. |
| PLATE READER | |||||
| Varioskan | $18 | $27 | per hour | CALENDAR | Contact Noelani Kamelamela for training |
| ROBOTICS | |||||
| Tecan EVO 150 | $50 | $55 | per 1h block - based on scheduled time. | CALENDAR | |
| non-filter tips | $6.00 | $6.00 | per box | ** Please let us know if there is additional plasticware you would like us to stock. | |
| Filter tips | $8.00 | $8.00 | per box | ||
| Training | $50 | $55 | per session | Contact Stuart | |
| NANODROP | |||||
| Nanodrop | -- | -- | Walk up service | * no charge | |
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES | unit | CORE LAB | Other MIT / non-MIT | Notes |
|---|---|---|---|---|
| Informatics Support | per hour | $80 | $110 / $150 | Very limited support for non-MIT users |
| Informatics Share | per 0.1FTE | $1,100 | N/A | MIT only. Priority access to informatics. |
| Active Data storage | per TB per year | $160 | $240 / N/A | |
| Archival Storage (read only. Unmutable) | per TB per year | $80 | $120 / N/A | |
| Tape Archive / Recovery | per tape | $60 | $70 |
