BioMicroCenter:PricingFY2022
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| HOME -- | SEQUENCING -- | LIBRARY PREP -- | HIGH-THROUGHPUT -- | COMPUTING -- | OTHER TECHNOLOGY |
SEQUENCING
Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.
PRICING UPDATE 7/1/2021
NOVASEQ SEQUENCING
pricing update effective 10/1/2020
| NOVASEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| NovaSeq: 400m reads | $1,600 | $1,760 | $2,240 | Per 400m reads (max 150PE) | Requires BMC library prep, Max 96 samples |
| NovaSeq SP 100nt | $3,365 | $3,701 | $4,711 | Per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| NovaSeq SP 300nt** | $5,200 | $5,720 | $7,280 | ||
| NovaSeq SP 500nt | $6,400 | $7,040 | $8,960 | ||
| NovaSeq S1 100nt** | $5,300 | $5,830 | $7,420 | ||
| NovaSeq S1 200nt** | $6,900 | $7,590 | $9,660 | ||
| NovaSeq S1 300nt** | $7,750 | $8,525 | $10,850 | ||
| NovaSeq S2 100nt** | $9,500 | $10,450 | $13,300 | ||
| NovaSeq S2 200nt** | $11,500 | $12,650 | $16,100 | ||
| NovaSeq S2 300nt** | $12,600 | $13,860 | $17,640 | ||
| NovaSeq S4 50nt | $3,500 | $3,850 | $4,900 | Per Lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| NovaSeq S4 300nt | $5,000 | $5,500 | $7,000 |
- - minimal inventory maintained
NEXTSEQ500 SEQUENCING
| NEXTSEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 91nt kit (eg. 40PE or 75SE) |
$2,270 | $2,497 | $3,178 | per flowcell | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 166nt kit (eg. 80PE or 150SE) |
$3,900 | $4,290 | $5,460 | ||
| 316nt kit (eg. 150PE or 300SE) |
$5,800 | $6,380 | $8,120 |
MISEQ SEQUENCING
| MISEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| 70nt V2 kit (eg. 30PE or 70SE) |
$1,250 | $1,375 | $1,750 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1 year |
| 150nt V3 kits (eg. 75PE) |
$1,350 | $1,485 | $1,890 | ||
| 300nt V2 kit (eg. 150PE) |
$1,475 | $1,623 | $2,100 | ||
| 500nt V2 kit (eg. 250PE) |
$1,703 | $1,874 | $2,310 | ||
| 600nt V3 kit (eg. 300PE) |
$2,043 | $2,248 | $2,898 | ||
| 500nt NANO Run (eg. 250PE) |
$730 | $803 | $1,022 |
HISEQ2000 SEQUENCING
The HiSeq2000 in the BioMicro Center is in the process of being retired. We strongly encourage users to move to one of the other sequencers if possible.
| HISEQ SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| Single End 40nt | $1,200 | $1,320 | $1,800 | per lane | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of FASTQ files for 1y. |
| Other read lengths | 7/1/18: Custom only. Requires purchase of full flowcell. | ||||
OTHER ILLUMINA SERVICES
| ILLUMINA SUPPORTING SERVICES | CORE LAB | MIT | Non-MIT | unit | Notes |
|---|---|---|---|---|---|
| Quality Control Only -only one QC is included per sequencing lane. | $20 | $26 | $30 | per sample | Includes RT-PCR and Frag.Analyzer |
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. | $10 | $13 | $15 | per sample | Includes Fluorometric quantification and Frag.Analyzer |
| qPCR only (Illumina) | $15 | $16.5 | $22.5 | per sample | Includes qPCR values from standard QC only |
| qPCR only - full plate(Illumina) | $300 | $330 | $450 | per sample | excludes column 1 (used for controls) |
LONG READ SEQUENCING
| SEQUEL SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes0 |
|---|---|---|---|---|---|
| 20 hour v3 Chemistry | $1,470 | $1,617 | $2,205 | per SMRTCell | Includes polymerase binding and movie. |
| PROMETHION SEQUENCING | CORE LAB | MIT | Non-MIT | unit | Notes |
| 72 hour Flowcell | $1,500 | $1,650 | $2,100 | per Flowcell | Fastq stored for 1y. Trace data stored only 30d. |
LIBRARY GENERATION
ILLUMINA LIBRARY - DNA
| ILLUMINA Sample Preparation | Type | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| STANDARD THROUGHPUT DNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT DNA Prices for sample batches. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included. | |||||
| LIGATION BASED NEB UltraII |
STD | per sample | $105 | $115 | $157 | Includes QC, adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA). |
| HTL | 24 | $790 | $869 | $1185 | Includes adapter ligation, size selection, barcoding, enrichment, and quality control spotcheck (SYBR and FA). | |
| 96 | $1575 | $1732 | $2362 | |||
| Tagmentation NEXTERA-XT |
STD | per Sample | $105 | $115 | $157 | Includes set-up, tagmentation, indexing, enrichment, and quality control (SYBR and FA). |
| Tagmentation NEXTERA-FLEX |
$157 | $173 | $236 | |||
| NEXTERA-XT OR FLEX | HTL | 24 | $790 | $869 | $1185 | Includes tagmentation, indexing, enrichment, and quality control spotcheck(SYBR and FA). |
| 96 | $1575 | $1732 | $2362 | |||
| 16S Amplicon | HTL | 48 | $1,890 | $2,080 | $2,835 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). |
| add'l 48 | $630 | $693 | $945 | |||
| Other Amplicon | HTL | 48 | $1,512 | $1,663 | $2,268 | Includes normalization, enrichment, pooling and quality control (SYBR and FA). Samples *must* be submitted amplified with appropriate linkers. |
| add'l 48 | $630 | $693 | $945 | |||
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||||
| High Throughput Setup | 48 samples | $50 | $55 | $75 | ||
| Sample Cleanup | per sample | $5 | $5.5 | $7.5 | SPRI cleanup of samples to remove impurities. | |
| Quantification | per sample | $5 | $5.5 | $7.5 | Quantification of samples (FA) prior to arraying. | |
ILLUMINA LIBRARY - RNA
| ILLUMINA Sample Preparation | Type | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| STANDARD RNA Prices for individual samples. Repreps will typically be attempted on failed samples without charge. |
HIGH THROUGHPUT RNA Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff. Initial QC not included | |||||
| polyA based isolation Kapa mRNA Hyperprep OR NEB UltraII RNAseq |
STD | per sample | $157 | $173 | $236 | Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA). |
| HTL (NEB only) |
24 | $1,310 | $1,444 | $1,969 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $2,620 | $2,888 | $3,937 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | ||
| High Throughput 3' Digital Gene Expression (HT3DGE) | HTL | 24 | $1050 | $1155 | $1575 | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC. |
| 96 | $2100 | $2310 | $3150 | |||
| Total RNA sequencing:Mammalian Kapa Hyperprep + RiboErase OR NEB RNAseq +Ribodepletion |
STD | per sample | $235 | $258.5 | $352.5 | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. |
| HTL (NEB only) |
24 | $2100 | $2310 | $3150 | Includes polyA isolation, generation of cDNA, library construction, indexing and spot QC (SYBR + FA). | |
| 96 | $4200 | $4620 | $6300 | |||
| Low Input polyA+ Clontech SMARTseq + NexteraXT or Flex |
STD v4 |
per sample | $262 | $289 | $394 | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC. |
| HTL v2 |
24 | $1680 | $1848 | $2520 | ||
| 96 | $2625 | $2888 | $3938 | |||
| Low Input Mammalian Total Clontech ZapR Kit |
STD | per sample | $210 | $231 | $315 | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). |
| Small RNA | STD | per sample | $289 | $318 | $433 | Uses NEB, BIOO or QIAGEN small RNA kit |
| ADDITIONAL FEES Samples not in proper input format will be subject to these additional charges. | ||||||
| High Throughput Setup | 48 samples | $50 | $55 | $75 | ||
| Sample Cleanup | per sample | $5 | $5.5 | $7.5 | SPRI cleanup of samples to remove impurities. | |
| Quantification | per sample | $5 | $5.5 | $7.5 | Quantification of samples (FA) prior to arraying. | |
.
LONG READ LIBRARIES
| PacBio Sample Preparation | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| SMRTBell Only eg:PCR products |
Sample 1 | $315 | $346 | $472 | Includes QC, repair and addition of indexed SMRTBells |
| Samples 2-8 | $157 | $173 | $236 | ||
| Genomic Library | Sample 1 | $472 | $520 | $709 | Includes Covaris gSPIN fragmentation, Pippin sizing, repair and SMRTbell as above |
| Sample 2-8 | $235 | $258 | $352 | ||
| Oxford Nanopore Sample Preparation | Nanopore Kit | CORE LAB | MIT | Non-MIT | Notes |
| Standard Ligation | LSK109 | $150 | $165 | $225 | Oxford Nanopore prep methods remain highly experimental and are continually in flux. As such, we can only estimate cost based on reagents/effort and cannot guarantee results. Please consult the core prior to sample submission for the latest information. |
| Native Barcoding (ligation indexing) |
- | $50 | $55 | $70 | |
| SMARTseq -> Ligation | Cv5+LSK109 | $315 | $346 | $472 | |
| Rapid Prep (tagment - ~10-20kb) | RAD004 | $157 | $173 | $236 | |
| Long Insert Prep | RAD004 (modified, target >100kb) | $262 | $289 | $394 | |
| Direct RNA | RNA002 | $210 | $231 | $315 |
SINGLE CELL & SPATIAL SERVICES
SEQWELL
| 10X USAGE | Batch Size | KI | OTHER CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| Assisted Service | ||||||
| FULL SERVICE Begin with cells |
per array | - | - | - | - | Includes all steps, QC of cDNA Begins in KI Nanowell Core |
| FROM cDNA on BEADS | per sample | $350 | $350 | $385 | $525 | Includes exonuclease treatment, amplification, QC of cDNA and library production Must coordinate with BMC staff on submission. |
| FROM AMPLIFIED cDNA | per sample | $100 | $100 | $110 | $150 | Includes QC of cDNA and library production only. |
| Reagents for SeqWell | ||||||
| SeqWell Array | contact shanu@mit.edu for pricing and availability | |||||
| SeqWell beads | ||||||
10X CHROMIUM
| 10X USAGE | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Assisted Service | |||||
| DIGITAL GENE EXPRESSION | |||||
| 5'RNA 3' RNA |
setup | $866 | $953 | $1299 | charge per submission |
| per sample | $1455 | $1601 | $2,037 | Includes QC of cDNA and creation of Illumina Library | |
| Amplicon (TCR, BCR, antibody barcocde // per amplicon) | setup | $367 | $404 | $551 | additional prep beyond DGE |
| per sample | $30 | $33 | $45 | Includes QC of cDNA and creation of Illumina Library | |
| scATACseq | |||||
| ATACseq | setup | $750 | $825 | $1125 | charge per submission |
| per sample | $1560 | $1716 | $2184 | Includes QC and creation of Illumina Library | |
| Multiome (ATAC+3'RNA) | |||||
| MULTI | setup | $1485 | $1633.5 | $2079 | charge per submission |
| per sample | $2575 | $2833 | $3605 | Includes QC of cDNA and creation of Illumina Library | |
| CANCELLATION | |||||
| per session | $200 | $220 | $280 | <24h notice | |
| Walk-up Service | |||||
| 3'/5'RNA Beads and Reagents (v3) | per samples | $1350 | $1485 | NA | |
| ATACseq Beads and Reagents* | per sample | $1450 | $1595 | NA | |
| multiome Beads and Reagents* | per sample | $2350 | $2585 | NA | |
| Chips (up to 8 sample) | per chip | $350 | $385 | NA | Includes service contract charge. |
| Training Limited to 2 people |
per method & per session Reagents not included |
$550 | $605 | NA | |
- please let BMC know if you plan to run this method early so we will have reagents ready for you.
NAMOCELL
| NAMOCELL | Batch Size | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Usage | Per run | $20 | $22 | $30 | per hour |
| Cartridge | 1 cartridge per sample | $60 | $66 | $90 |
VISIUM
| SLIDES | Batch Size | KI Only | Other CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|---|
| Test Array | slide | $1157.75 | $1501.5 | $1510.5 | $2216 | recommended 1x per tissue type |
| Sample Slide | 1 slide / 4 tiles | $5480 | $5614 | $6200 | $7672 | Includes H&E stain images + Library Generation |
OTHER GENOMICS SERVICES
QUALITY CONTROL
| QUALITY CONTROL | unit | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| Fragment Analyzer: RNA or DNA | per sample | $8 | $9 | $12 | |
| Fragment Analyzer: Preloaded row | per row (12) | $44 | $48 | $66 | 2ul per sample in each well using plates from BMC |
| FemtoPulse: RNA or DNA | per sample | $12 | $13 | $18 | |
| FemtoPulse: Preloaded Row | per row (12) | $66 | $73 | $99 | 2ul per sample in each well using plates from BMC |
| BioAnalyzer: Assisted Service (RNA or DNA) | per sample | $15 | $18 | $25 | |
| BioAnalyzer: Assisted Service (High Sensitivity protein) | per sample | $40 | $44 | $60 | detection of proteins at < 30ng/ul. |
| qPCR only (Illumina quantification test) | per sample | $20 | $22 | $30 | 4 point serial dilution |
| Full plate qPCR (Illumina quantification test) | per plate | $300 | $330 | $450 | full plate only (exclude column 1 for standards) - must be to BMC specifications. |
MICROARRAYS
| MICROARRAYS | unit | CORE LAB | MIT | Non-MIT | Notes |
|---|---|---|---|---|---|
| AGILENT SCANNER | per slide | $50 | $50 | N/A | Price per glass slide. |
Agilent scanning services will end 6/30/2019
.
REAL TIME PCR
| OTHER LAB SERVICES | CORE LAB / MIT | Non-MIT | unit | SIGN UP | Notes |
|---|---|---|---|---|---|
| Roche LightCycler 480 | $10 | N/A | per plate | CALENDAR *available for CORE only | Contact Noelani Kamelamela for training |
| SYBR Green (KAPA/Roche) | $36 | N/A | per ml | Available for MIT only | |
| 96-well PLATES | $6 | N/A | per plate | Available for MIT only |
.
OTHER LAB SERVICES
| OTHER LAB SERVICES | CORE LAB / MIT | Non-MIT | unit | SIGN UP | Notes |
|---|---|---|---|---|---|
| COVARIS SONICATOR | |||||
| COVARIS E220 | $20 | $30 | per hour | ilab calendar | tubes available for purchase. |
| OLIGO SYNTHESIS | |||||
| Assisted | $1000 +consumables |
$1500 +consumables |
per run | includes setup and run of the instrument only. | |
| Walkup | $500 +consumables |
NA | per run | includes =run of the instrument only. | |
| Training | $2000 | N/A | includes two instrument runs. | ||
| PIPPIN PREP | |||||
| Gel Isolation | $50 | $75 | per gel (5 lanes) | TBA | |
| Training | $25 | $50 | per session | TBA | Contact Noelani Kamelamela for training. |
| PLATE READER | |||||
| Varioskan | $18 | $27 | per hour | CALENDAR | Contact Noelani Kamelamela for training |
| ROBOTICS | |||||
| Tecan EVO 150 | $50 | $55 | per 1h block - based on scheduled time. | CALENDAR | |
| non-filter tips | $6.00 | $6.00 | per box | ** Please let us know if there is additional plasticware you would like us to stock. | |
| Filter tips | $8.00 | $8.00 | per box | ||
| Training | $50 | $55 | per session | Contact Austin Hendricks | |
| NANODROP | |||||
| Nanodrop | -- | -- | Walk up service | * no charge | |
COMPUTATIONAL SERVICES
| COMPUTATIONAL SERVICES | unit | CORE LAB | Other MIT / non-MIT | Notes |
|---|---|---|---|---|
| Informatics Support | per hour | $85 | $115 / $160 | Very limited support for non-MIT users |
| Informatics Share | per 0.1FTE | $1,200 | N/A | MIT only. Priority access to informatics. |
| Ingenuity Pathway Analysis | per dataset | $100 | $110/$150 | Recovery of licence cost. |
| Active Data storage | per TB per year | $125 | $300 / N/A | |
| Archival Storage (read only. Unmutable) | per TB per year | $75 | $150 / N/A | |
| Tape Archive / Recovery | per tape | $60 | $70 |
