AVM BIOL368 Week 2

Molecular Biology

Purpose

The purpose of this assignment was to find an amino acid sequence that corresponded with a true breeding purple flower.

Method and Results

• Protocol followed: Part III: Molecular Biology

1. Click on the tab in Aipotu labeled "Molecular Biology"
2. Under "Greenhouse" choose flower Green-2, note that one allele is gene and the other is yellow.
3. Click the tab "Compare" and choose "Upper vs. Lower", the results are pictured below.

1. Repeat the compare tab for the Green-1, Red and White flowers. Results of changes in sequences pictured below.

1. Compare two different White Flowers (not all white alleles have the same DNA sequence). Results below.

1. Make the protein: MLVKEIAMYRFATHER and click "Fold Protein"
   • Note that this creates a purple flower
2. Click "Enter New DNA" in the lower gene window and repeat the same sequence

• Results pictured below

1. Compare the upper and lower sequences for the purple flower to prove that it is pure-breeding. Results below.

Scientific Conclusions

• It was found that the starting flowers: Green-1, Green-2, Red and White all had the amino acid sequence beginning with: MetSerAsnArgHisIleLeuLeuVal. Each sequence varied there after.
• The true-breeding purple flower had the amino acid sequence of N-MetLeuValLysGluIleAlaMetTyrArgPheAlaThrHisGluArg-C which varied quite a bit more than the other colors.
• It was also noted that there was the ability to breed flowers with the colors yellow, orange, black and blue as well.
• It was found that mutations occurred in the DNA sequences of many of the white flowers and if the promoter and terminator sequences were affected.

Acknowledgements

• Worked in collaboration with Isai_Lopez and Matthew Oki
• Template followed from week 2 assignment BIOL368/F16
• We had help from our professor, Dr. Dahlquist in class as well to complete this assignment.
• Collaborated with Biochemistry group member Mia Huddleston while determining the amino acid sequence

Note: While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source.

Avery Vernon-Moore 01:13, 7 September 2016 (EDT)

References

• Website used: Aipotu
  • All images and information were created using the Aipotu application

Useful Links:

• User: Avery_Vernon-Moore

User: Avery_Vernon-Moore

Weekly Assignments:

Assignment 1
Assignment 2
Assignment 3
Assignment 4
Assignment 5
Assignment 6
Assignment 7
Assignment 8
Assignment 9
Assignment 10
Assignment 11
Assignment 12
Assignment 14
Assignment 15

Individual Journals:

"Week 1: Create Wiki Page" 
Individual Journal 2
Individual Journal 3
Individual Journal 4
Individual Journal 5
Individual Journal 6
Individual Journal 7
Individual Journal 8
Individual Journal 9
Individual Journal 10
Individual Journal 11
Individual Journal 12
No Week 13 Assignment
Individual Journal 14
Individual Journal 15

Shared Journals:

Class Journal 1
Class Journal 2
Class Journal 3
Class Journal 4
Class Journal 5
Class Journal 6
Class Journal 7
Class Journal 8
Class Journal 9
Class Journal 10
Class Journal 11
Class Journal 12
Class Journal 14
Class Journal 15

• BIOL368/F16 Week 2