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- 00:31, 26 March 2024 Insect Nutrition and Metabolism talk contribs created page Orgenizing the data from the LCMS mechine (Created page with "* In order to organize the data for further analysis , we use script in R * Please go over the presentations File:presentation1.ppt, File:presentation2.ppt, File:presentation2.ppt")
- 00:19, 26 March 2024 Insect Nutrition and Metabolism talk contribs created page File:Standard curve.png
- 00:19, 26 March 2024 Insect Nutrition and Metabolism talk contribs uploaded File:Standard curve.png
- 00:10, 26 March 2024 Insect Nutrition and Metabolism talk contribs created page File:Melting curve.png
- 00:10, 26 March 2024 Insect Nutrition and Metabolism talk contribs uploaded File:Melting curve.png
- 00:08, 26 March 2024 Insect Nutrition and Metabolism talk contribs created page File:Melting curve2.png
- 00:08, 26 March 2024 Insect Nutrition and Metabolism talk contribs uploaded File:Melting curve2.png
- 00:05, 26 March 2024 Insect Nutrition and Metabolism talk contribs created page File:Meting curve.png
- 00:05, 26 March 2024 Insect Nutrition and Metabolism talk contribs uploaded File:Meting curve.png
- 23:19, 25 March 2024 Insect Nutrition and Metabolism talk contribs created page File:Melting curve.pdf
- 23:19, 25 March 2024 Insect Nutrition and Metabolism talk contribs uploaded File:Melting curve.pdf
- 11:02, 25 March 2024 Insect Nutrition and Metabolism talk contribs created page Designing and testing the qPCR primers (Created page with "# For additional information [https://www.dropbox.com/scl/fi/070fhrrw6heimv1xxuxzq/real-time-pcr-handbook-life-technologies-update-flr.pdf?rlkey=eddim7471x9l8uqww1234yg6w&dl=0] ===House keeping genes=== # When checking gene expressed in the BSF it is important to use housekeeping gene as a reference. # Housekeeping genes, also known as reference genes, are a set of genes that are constitutively expressed in cells and are essential for basic cellular functions. # T...")
- 08:54, 28 January 2024 Insect Nutrition and Metabolism talk contribs created page File:RNA bands.jpeg
- 08:54, 28 January 2024 Insect Nutrition and Metabolism talk contribs uploaded File:RNA bands.jpeg
- 08:50, 28 January 2024 Insect Nutrition and Metabolism talk contribs created page Checking the RNA integrity on gel electrophoresis (Created page with "===Preparation of the gel=== * Prepare a 1.5-2% gel electrophoresis. * For example, for a small gel, add 0.53 g agarose and 35 ml TBE/TAE to an Erlenmeyer flask. * Microwave for 30-45 seconds, stop and swirl, and then continue towards a boil until the agarose is completely dissolved. * Let the agarose solution cool down and add 3 µl of 'redsafe' (the amount depends on the 'redsafe' company). * Pour the agarose into a gel tray with the well comb in place. * Use the s...")
- 08:39, 28 January 2024 Insect Nutrition and Metabolism talk contribs created page How to interpret nano-drop results (Created page with "===260/280 Ratio=== * The ratio of absorbance at 260 nm and 280 nm is used to assess the purity of DNA and RNA. * A ratio of ~2.0 is generally accepted as 'pure' for RNA. * If the ratio is appreciably lower in either case, it may indicate the presence of protein, phenol, or other contaminants that absorb strongly at or near 280 nm. ===260/230 Ratio=== *This ratio is used as a secondary measure of nucleic acid purity. * Expected 260/230 values are commonly in the ra...")
- 04:36, 19 December 2023 Insect Nutrition and Metabolism talk contribs created page Analysis LCMS results (Created page with "* Workflow for analysis using MetaboAnalyst [https://www.dropbox.com/scl/fi/7tcri1zvj5zlbaf5u2xjr/Metaboanalyst-workflow.docx?rlkey=vf2b9771nic2dgmjnnsx70ymm&dl=0] * Information about MetaboAnalyst [https://www.dropbox.com/scl/fi/w8ldsd44jod8ahbxksftc/MEtaboanalyst-5.pdf?rlkey=jbhtvlxy2fp22msfcpgxpjfmz&dl=0]")
- 04:24, 19 December 2023 Insect Nutrition and Metabolism talk contribs created page Analysing NGS (Created page with "* R script [https://www.dropbox.com/scl/fi/jyndvcutv5p3wkrx9yr6v/Itai_Dada2_unite.R?rlkey=pgdvz039mjhvsocprx91vs469&dl=0]")
- 04:02, 19 December 2023 Insect Nutrition and Metabolism talk contribs created page How to interoperate nano-drop results (Created page with "[https://www.dropbox.com/scl/fi/38zhpeuyzf4m6dfbd0b86/InterpretingSpecNanodrop.pdf?rlkey=cgb5t71cwgqqcaxxrksie2seu&dl=0]")
- 08:45, 26 November 2023 Insect Nutrition and Metabolism talk contribs created page YPD broth (Created page with "===Safety measures=== *'''when working with yeast - work only in the biological hoog - for your safety'''")
- 08:39, 26 November 2023 Insect Nutrition and Metabolism talk contribs created page Standard diet (Created page with "* 50% wheat bran * 30% Alfalfa * 20% Corn-flour Based on [https://academic.oup.com/jee/article-abstract/85/6/2291/846608]")
- 02:13, 8 June 2023 Insect Nutrition and Metabolism talk contribs created page CDNA synthesis (Created page with "* Protocol file [https://www.dropbox.com/s/1mk1u26398f80oj/cDNA synthesis protocol.xlsx?dl=0] ===Protocol=== # Measure the RNA amounts (ng/ul) using the nanodrop # Calculate the amount of RNA to be used in each reaction (use the file above)")
- 02:24, 24 May 2023 Insect Nutrition and Metabolism talk contribs created page RNA samples purification (Created page with "==Materials== * DNAse ==Protocols== * Protocol for RNA Cleanup and Concentration Using A&A Biotechnology kit [https://www.dropbox.com/s/ircnq2d06odfrog/Clean-Up-RNA-Concentrator-EN.pdf?dl=0] ==DNA purification form== [https://www.dropbox.com/s/szmyicqx8f3xlt3/RNA clean up.xlsx?dl=0]")
- 04:53, 16 May 2023 Insect Nutrition and Metabolism talk contribs created page Manual protocol (Created page with " מאמר 1 dx.doi.org/10.17504/protocols.io.2yfgftn אנא זכרו לצטט עם הפרוטוקול- https://www.ncbi.nlm.nih.gov/pubmed/28856640 ציטוט פרוטוקול- Benjamin Schwessinger 2019. High quality DNA from Fungi for long read sequencing e.g. PacBio. protocols.iohttps://dx.doi.org/10.17504/protocols.io.2yfgftn הפקת DNA באיכות גבוה לרצף PacBio מותאם להפקת DNA מנבגי חלודה מחיטה ומשעורה. הכי...")
- 04:52, 16 May 2023 Insect Nutrition and Metabolism talk contribs created page QiaGen's kit protocol (Created page with "מידע מהhandbook: הקיט מיועד לבידוד DNA כרומוזומלי בגדול של 20-150 kb (איור 1 עמוד 8) מתוך עמוד 13- הקיט מכיל בופרים שממיסים את מרכיבי התא, הכל קורה על גבי המסננת של הטיפ 20/G שעשוי שרף וקושר את הDNA בתנאי מלח וPH מתאימים, לכן יש להימנע מייבוש המסננת. חלבונים וזיהומים בעלי משקל...")
- 04:49, 16 May 2023 Insect Nutrition and Metabolism talk contribs created page DNA extraction for genome sequencing (Created page with "==Manual protocol== ==QiaGen's kit protocol==")
- 04:44, 16 May 2023 Insect Nutrition and Metabolism talk contribs created page DNA samples purification (Created page with "==Protocols== * Protocol for DNA Cleanup and Concentration Using the Monarch [https://www.dropbox.com/s/fh441z99va5h54y/Monarch_PCRDNA_Cleanup.pdf?dl=0] * Wizard® SV Gel and PCR Clean-Up System [https://www.dropbox.com/s/0iekonqwxtny5np/Wizard SV Gel and PCR Clean-up System Quick Protocol FB072.pdf?dl=0]")
- 10:44, 13 May 2023 Insect Nutrition and Metabolism talk contribs created page Extraction of RNA (Created page with "===Safety measures=== * '''After adding the chloroform, the work should be conducted in the chemical hood''' ==RNA extraction protocol== * Hybrid-R [https://www.dropbox.com/s/6s5yniyuq3sfrmu/Hybrid-R protocol.pdf?dl=0] ==RNA extraction form== * [https://www.dropbox.com/s/6s5yniyuq3sfrmu/Hybrid-R protocol.pdf?dl=0]")
- 21:30, 10 May 2023 Insect Nutrition and Metabolism talk contribs created page Amino acids composition (Created page with "* The samples should be freeze-dried (4-10 mg of dried sample) * An account should be open at the Research Core Facilities Program at UC Davis [https://ppms.us/ucdavis/login/?pf=8] * Please print out this e-mail and include it with your samples. If you have a IOC/Recharge # (UC) or a PO # please note, or if you would rather be invoiced (and pay by credit card) please indicate here Please do not hesitate to call (530-752-7327 ) or e-mail (msf@ucdavis.edu) if you h...")
- 22:19, 8 May 2023 Insect Nutrition and Metabolism talk contribs created page Extraction of DNA (Created page with "==Protocols of extraction kits== * QiaGen")
- 06:13, 3 May 2023 Insect Nutrition and Metabolism talk contribs created page Sample preparation for LCMS (Created page with "# 10 BSF larvae from each replicate and treatment should ground with liquid nitrogen. 2. The larval powder should be freezed immediately in -80°C until lyophilization. 3. After lyophilization, 100 mg of larval powder dissolved in 700 microliters of precooled metabolite extraction buffer (80% methanol and 0.1% formic acid), resuspended by vortex, incubated on ice for 5 min at 4 °C. (Metabolite extraction buffer: To prepare working stock for 50 ml of extraction buffer,...")
- 06:09, 3 May 2023 Insect Nutrition and Metabolism talk contribs created page Sample dilution (Created page with "===Equipment=== * Brown veils and cups * Inserts ===Materials=== * benzophenone * Petroleum ether ==Protocol== ===Solutions A=== For 30 ml - 29.7 ml petroleum ether + 300 ul benzophenone ===Solution B=== For 30 ml - 25 ml '''solution A''' + 25 ml petroleum ether ===Sample dilution=== * Each sample should be diluted to two concentrations: 1:125 and 1:5000 * From each sample 8 ul will be dilutes with 992 ul '''solution B''' (for 1:125 dilution) * From each sample 25 ul...")
- 05:56, 3 May 2023 Insect Nutrition and Metabolism talk contribs created page Calibration curve (Created page with "===Equipment=== * Brown veils and cups * Inserts ===Materials=== * benzophenone * Petroleum ether ==Protocol== ===Solutions A=== for 30 ml - 29.7 ml petroleum ether + 300 ul benzophenone ===Solution B===")
- 05:51, 2 May 2023 Insect Nutrition and Metabolism talk contribs created page Fatty acids composition (Created page with "===Safety measures=== * '''When preparing the Sulfuric acid - to do it the chemical hood (with lowered glass) with safety glasses and thick gloves''' * '''Pour the acid into the methanol in cooled (with ice) bucket''' * '''The work should be conducted in chemical hood''' * '''When dealing with hot equipment and sulfuric acid - wear thick gloves''' ===Equipment=== * '''before working with the Gooch make sure the clean them in the dish washer, burn them in 600 degrees an...")
- 08:08, 7 March 2023 Insect Nutrition and Metabolism talk contribs created page Ash and Fiber content (Created page with "===Safety measures=== * "''When preparing the Sulfuric acid - to do it the chemical hood (with lowered glass) with safety glasses and thick gloves''' * '''Pour the acid into the methanol in cooled (with ice) bucket''' * '''The work should be conducted in chemical hood''' * '''When dealing with hot equipment and sulfuric acid - wear thick gloves''' ===Equipment=== * Analytical balance * '''before working with the Gooch make sure the clean them in the dish washer, burn t...")
- 07:44, 7 March 2023 Insect Nutrition and Metabolism talk contribs created page File:Nitrogen.png
- 07:44, 7 March 2023 Insect Nutrition and Metabolism talk contribs uploaded File:Nitrogen.png
- 13:30, 27 February 2023 Insect Nutrition and Metabolism talk contribs created page Protein Content (Created page with "===Safety measures=== * '''The work should be conducted using the FOSS Kjeltec 8100 in the analytical laboratory at Tel-Hai''' * '''The work should be conducted in chemical hood''' * '''When dealing with hot equipment and sulfuric acid - wear thick gloves''' ===Equipment=== * Analytical balance * Kjeldahl tubes (at the number of samples) ===Materials=== * NaOH (0.1M) * HCl (0.1M) * Sulfuric acid * Hydrogen peroxide (30%) ==Protocol== # Weight approx. 0.2 gr of material...")
- 01:12, 26 February 2023 Insect Nutrition and Metabolism talk contribs created page Fat content (Created page with "===Equipment=== * Analytical balance * Soxhlet system * Cooling system (with ice!!!) * Evaporator * 250 ml flask * 50 ml chemical glass ===Materials=== * Hexan ==Protocol #")
- 05:33, 23 February 2023 Insect Nutrition and Metabolism talk contribs created page Laboratory for insect nutrition and metabolomics (Created page with "==Chemical analysis==")
- 03:59, 23 February 2023 Insect Nutrition and Metabolism talk contribs created page User:Insect Nutrition and Metabolism (Created page with "Welcome to the WIKI page of the Insect Nutrition and Metabolism laboratory")