Preparing the plate

• First, plan your plate, then prepare your SYBR-primer mix, and dilute your DNA accordingly.
• An example for a planned plate: Usually three technical triplicates are used.

File:PCR plate example.pdf

• NTC non template control. Allows detection of contamination of the PCR reagents. An NTC reaction contains all real-time PCR components except the template (DNA). Instead, put DDW water.
• Standard - for more details see section: Prepering a standard curve to quantify the total yeast