CDNA synthesis
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- Protocol file [[[1]]]
Protocol
- Measure the RNA amounts (ng/ul) using the nanodrop
- Calculate the amount of RNA to be used in each reaction (use the file above)
- Calculate the amounts of the other components of the reaction (use the file above)
- Run the PCR according to the program of:
- 42°C for 30 min
- 85°C for 10 min
- 4°C forever
