CDNA synthesis

From OpenWetWare
Jump to navigationJump to search
  • Protocol file [[[1]]]


  1. Measure the RNA amounts (ng/ul) using the nanodrop
  2. Calculate the amount of RNA to be used in each reaction (use the file above)
  3. Calculate the amounts of the other components of the reaction (use the file above)
  4. Run the PCR according to the program of:
  • 42°C for 30 min
  • 85°C for 10 min
  • 4°C forever