Calibration curve

Equipment

• Brown veils and cups
• Inserts

Materials

• benzophenone
• Petroleum ether
• fatty acid mix (sigma) [[[1]]]

Protocol

Solutions A

For 30 ml - 29.7 ml petroleum ether + 300 ul benzophenone

Solution B

For 30 ml - 25 ml solution A + 25 ml petroleum ether

Calibration solution (in inserts)

• Blank - 1 ml petroleum ether
• Blank 2 - 1 ml solution B
• 1:2 dilution - 100 ul solution A + 100 ul FAME mix
• 1:4 dilution - 100 ul 1:2 dilution + 100 ul solution B
• 1:8 dilution - 100 ul 1:4 dilution + 100 ul solution B
• 1:16 dilution - 100 ul 1:8 dilution + 100 ul solution B
• 1:32 dilution - 100 ul 1:16 dilution + 100 ul solution B
• 1:64 dilution - 100 ul 1:32 dilution + 100 ul solution B