Sample dilution
From OpenWetWare
Jump to navigationJump to search
Equipment
- Brown veils and cups
- Inserts
Materials
- benzophenone
- Petroleum ether
Protocol
Solutions A
For 30 ml - 29.7 ml petroleum ether + 300 ul benzophenone
Solution B
For 30 ml - 25 ml solution A + 25 ml petroleum ether
Sample dilution
- Each sample should be diluted to two concentrations: 1:125 and 1:5000
- From each sample 8 ul will be dilutes with 992 ul solution B (for 1:125 dilution)
- From each sample 25 ul (from the 1:125 dilution) will be dilutes with 975 ul solution B (for 1:5000 dilution)
