User contributions for Insect Nutrition and Metabolism

24 May 2023

• 02:3202:32, 24 May 2023 diff hist +161‎ Manual protocol ‎No edit summary current
• 02:2402:24, 24 May 2023 diff hist +297‎ N RNA samples purification ‎ Created page with "==Materials== * DNAse ==Protocols== * Protocol for RNA Cleanup and Concentration Using A&A Biotechnology kit [https://www.dropbox.com/s/ircnq2d06odfrog/Clean-Up-RNA-Concentrator-EN.pdf?dl=0] ==DNA purification form== [https://www.dropbox.com/s/szmyicqx8f3xlt3/RNA clean up.xlsx?dl=0]" current

17 May 2023

• 05:5705:57, 17 May 2023 diff hist +6‎ Manual protocol ‎No edit summary
• 05:5605:56, 17 May 2023 diff hist +180‎ Manual protocol ‎No edit summary
• 05:5105:51, 17 May 2023 diff hist +260‎ Manual protocol ‎No edit summary

16 May 2023

• 06:2606:26, 16 May 2023 diff hist +160‎ Manual protocol ‎No edit summary
• 06:2206:22, 16 May 2023 diff hist +263‎ Manual protocol ‎No edit summary
• 06:0506:05, 16 May 2023 diff hist −11,678‎ Manual protocol ‎ Replaced content with "===Safety procedure=== ===Materials=== * 0.35 M sorbitol * 0.1 M Tris-HCL, pH9 * 5 mM EDTA, pH8 * 2 M NaCl * 2% CTAB (Cetrimonium bromide) * Sarkosyl (N-lauroylsarcosine sodium salt) * Polyvinylpyrrolidone (PVP) (40000 MW) 1 % [w/v] - ===Equipment=== ==Buffer A== * 0.35 M sorbitol * 0.1 M Tris-HCL, pH9 * 5 mM EDTA, pH8 '''Autoclave to sterilize''' ==Buffer B== * 0.2 M Tris-HCL, pH9 * 50 mM EDTA, pH8 * 2 M NaCl * 2% CTAB '''Autoclave to sterilize''' ==Buffer C== *..." Tag: Replaced
• 06:0406:04, 16 May 2023 diff hist +342‎ Manual protocol ‎No edit summary
• 05:5605:56, 16 May 2023 diff hist −273‎ Manual protocol ‎No edit summary
• 04:5304:53, 16 May 2023 diff hist +12,277‎ N Manual protocol ‎ Created page with " מאמר 1 dx.doi.org/10.17504/protocols.io.2yfgftn אנא זכרו לצטט עם הפרוטוקול- https://www.ncbi.nlm.nih.gov/pubmed/28856640 ציטוט פרוטוקול- Benjamin Schwessinger 2019. High quality DNA from Fungi for long read sequencing e.g. PacBio. protocols.iohttps://dx.doi.org/10.17504/protocols.io.2yfgftn הפקת DNA באיכות גבוה לרצף PacBio מותאם להפקת DNA מנבגי חלודה מחיטה ומשעורה. הכי..."
• 04:5204:52, 16 May 2023 diff hist +15,732‎ N QiaGen's kit protocol ‎ Created page with "מידע מהhandbook: הקיט מיועד לבידוד DNA כרומוזומלי בגדול של 20-150 kb (איור 1 עמוד 8) מתוך עמוד 13- הקיט מכיל בופרים שממיסים את מרכיבי התא, הכל קורה על גבי המסננת של הטיפ 20/G שעשוי שרף וקושר את הDNA בתנאי מלח וPH מתאימים, לכן יש להימנע מייבוש המסננת. חלבונים וזיהומים בעלי משקל..." current
• 04:4904:49, 16 May 2023 diff hist +53‎ N DNA extraction for genome sequencing ‎ Created page with "==Manual protocol== ==QiaGen's kit protocol==" current
• 04:4804:48, 16 May 2023 diff hist 0‎ DNA samples purification ‎No edit summary current
• 04:4804:48, 16 May 2023 diff hist +160‎ DNA samples purification ‎No edit summary
• 04:4404:44, 16 May 2023 diff hist +332‎ N DNA samples purification ‎ Created page with "==Protocols== * Protocol for DNA Cleanup and Concentration Using the Monarch [https://www.dropbox.com/s/fh441z99va5h54y/Monarch_PCRDNA_Cleanup.pdf?dl=0] * Wizard® SV Gel and PCR Clean-Up System [https://www.dropbox.com/s/0iekonqwxtny5np/Wizard SV Gel and PCR Clean-up System Quick Protocol FB072.pdf?dl=0]"
• 04:3904:39, 16 May 2023 diff hist +16‎ Laboratory for insect nutrition and metabolomics ‎No edit summary current
• 04:3804:38, 16 May 2023 diff hist +19‎ Laboratory for insect nutrition and metabolomics ‎No edit summary

13 May 2023

• 10:4410:44, 13 May 2023 diff hist +329‎ N Extraction of RNA ‎ Created page with "===Safety measures=== * '''After adding the chloroform, the work should be conducted in the chemical hood''' ==RNA extraction protocol== * Hybrid-R [https://www.dropbox.com/s/6s5yniyuq3sfrmu/Hybrid-R protocol.pdf?dl=0] ==RNA extraction form== * [https://www.dropbox.com/s/6s5yniyuq3sfrmu/Hybrid-R protocol.pdf?dl=0]" current

10 May 2023

• 21:4221:42, 10 May 2023 diff hist +21‎ Laboratory for insect nutrition and metabolomics ‎No edit summary
• 21:3621:36, 10 May 2023 diff hist −880‎ Amino acids composition ‎No edit summary current
• 21:3421:34, 10 May 2023 diff hist −1,285‎ Amino acids composition ‎No edit summary
• 21:3021:30, 10 May 2023 diff hist +2,732‎ N Amino acids composition ‎ Created page with "* The samples should be freeze-dried (4-10 mg of dried sample) * An account should be open at the Research Core Facilities Program at UC Davis [https://ppms.us/ucdavis/login/?pf=8] * Please print out this e-mail and include it with your samples. If you have a IOC/Recharge # (UC) or a PO # please note, or if you would rather be invoiced (and pay by credit card) please indicate here Please do not hesitate to call (530-752-7327 ) or e-mail (msf@ucdavis.edu) if you h..."

9 May 2023

• 02:1702:17, 9 May 2023 diff hist +101‎ Extraction of DNA ‎No edit summary current
• 02:1302:13, 9 May 2023 diff hist +190‎ Extraction of DNA ‎No edit summary

8 May 2023

• 22:2222:22, 8 May 2023 diff hist +64‎ Extraction of DNA ‎No edit summary
• 22:1922:19, 8 May 2023 diff hist +41‎ N Extraction of DNA ‎ Created page with "==Protocols of extraction kits== * QiaGen"
• 22:1822:18, 8 May 2023 diff hist +42‎ Laboratory for insect nutrition and metabolomics ‎No edit summary
• 05:1205:12, 8 May 2023 diff hist +17‎ Laboratory for insect nutrition and metabolomics ‎No edit summary

3 May 2023

• 06:3706:37, 3 May 2023 diff hist +8‎ Sample preparation for LCMS ‎No edit summary current
• 06:2606:26, 3 May 2023 diff hist +264‎ Sample preparation for LCMS ‎No edit summary
• 06:1306:13, 3 May 2023 diff hist +4,903‎ N Sample preparation for LCMS ‎ Created page with "# 10 BSF larvae from each replicate and treatment should ground with liquid nitrogen. 2. The larval powder should be freezed immediately in -80°C until lyophilization. 3. After lyophilization, 100 mg of larval powder dissolved in 700 microliters of precooled metabolite extraction buffer (80% methanol and 0.1% formic acid), resuspended by vortex, incubated on ice for 5 min at 4 °C. (Metabolite extraction buffer: To prepare working stock for 50 ml of extraction buffer,..."
• 06:1006:10, 3 May 2023 diff hist +34‎ Laboratory for insect nutrition and metabolomics ‎No edit summary
• 06:0906:09, 3 May 2023 diff hist +573‎ N Sample dilution ‎ Created page with "===Equipment=== * Brown veils and cups * Inserts ===Materials=== * benzophenone * Petroleum ether ==Protocol== ===Solutions A=== For 30 ml - 29.7 ml petroleum ether + 300 ul benzophenone ===Solution B=== For 30 ml - 25 ml '''solution A''' + 25 ml petroleum ether ===Sample dilution=== * Each sample should be diluted to two concentrations: 1:125 and 1:5000 * From each sample 8 ul will be dilutes with 992 ul '''solution B''' (for 1:125 dilution) * From each sample 25 ul..." current
• 06:0506:05, 3 May 2023 diff hist +16‎ Calibration curve ‎No edit summary current
• 06:0406:04, 3 May 2023 diff hist +438‎ Calibration curve ‎No edit summary
• 05:5905:59, 3 May 2023 diff hist +213‎ Calibration curve ‎No edit summary
• 05:5605:56, 3 May 2023 diff hist +206‎ N Calibration curve ‎ Created page with "===Equipment=== * Brown veils and cups * Inserts ===Materials=== * benzophenone * Petroleum ether ==Protocol== ===Solutions A=== for 30 ml - 29.7 ml petroleum ether + 300 ul benzophenone ===Solution B==="
• 05:5105:51, 3 May 2023 diff hist +51‎ Fatty acids composition ‎No edit summary current
• 05:4305:43, 3 May 2023 diff hist +37‎ Laboratory for insect nutrition and metabolomics ‎No edit summary
• 05:4205:42, 3 May 2023 diff hist −6‎ Laboratory for insect nutrition and metabolomics ‎No edit summary
• 05:4005:40, 3 May 2023 diff hist +647‎ Fatty acids composition ‎No edit summary

2 May 2023

• 06:5306:53, 2 May 2023 diff hist −773‎ Fatty acids composition ‎No edit summary
• 06:5106:51, 2 May 2023 diff hist −244‎ Fatty acids composition ‎No edit summary
• 05:5105:51, 2 May 2023 diff hist +1,768‎ N Fatty acids composition ‎ Created page with "===Safety measures=== * '''When preparing the Sulfuric acid - to do it the chemical hood (with lowered glass) with safety glasses and thick gloves''' * '''Pour the acid into the methanol in cooled (with ice) bucket''' * '''The work should be conducted in chemical hood''' * '''When dealing with hot equipment and sulfuric acid - wear thick gloves''' ===Equipment=== * '''before working with the Gooch make sure the clean them in the dish washer, burn them in 600 degrees an..."

8 March 2023

• 01:4001:40, 8 March 2023 diff hist +21‎ Ash and Fiber content ‎No edit summary current
• 01:3801:38, 8 March 2023 diff hist +23‎ Fat content ‎No edit summary current
• 01:3801:38, 8 March 2023 diff hist +524‎ Ash and Fiber content ‎No edit summary
• 01:2801:28, 8 March 2023 diff hist +491‎ Ash and Fiber content ‎No edit summary
• 01:0701:07, 8 March 2023 diff hist +75‎ Ash and Fiber content ‎No edit summary