User contributions for Insect Nutrition and Metabolism

9 September 2024

• 01:1401:14, 9 September 2024 diff hist +4‎ Prepering a standard curve to quantify the total yeast ‎No edit summary current
• 01:1301:13, 9 September 2024 diff hist +342‎ Prepering a standard curve to quantify the total yeast ‎No edit summary
• 01:1301:13, 9 September 2024 diff hist −1‎ Prepering a standard curve to quantify the total yeast ‎No edit summary
• 01:1201:12, 9 September 2024 diff hist +1‎ Prepering a standard curve to quantify the total yeast ‎No edit summary
• 01:1101:11, 9 September 2024 diff hist 0‎ N File:Standarde curve.png ‎No edit summary current
• 01:1101:11, 9 September 2024 diff hist +29‎ N Prepering a standard curve to quantify the total yeast ‎ Created page with "Image:Standarde_curve.png"
• 01:0801:08, 9 September 2024 diff hist +2‎ Preforming the PCR ‎No edit summary current Tag: Manual revert
• 01:0701:07, 9 September 2024 diff hist −4‎ Preforming the PCR ‎No edit summary
• 01:0701:07, 9 September 2024 diff hist +2‎ Preforming the PCR ‎No edit summary
• 01:0601:06, 9 September 2024 diff hist +27‎ Preforming the PCR ‎No edit summary
• 01:0401:04, 9 September 2024 diff hist 0‎ N File:PCR protocol.pdf ‎No edit summary current
• 01:0201:02, 9 September 2024 diff hist +133‎ Preforming the PCR ‎No edit summary
• 00:5900:59, 9 September 2024 diff hist +386‎ Preforming the PCR ‎No edit summary
• 00:5700:57, 9 September 2024 diff hist +501‎ N Preforming the PCR ‎ Created page with "===Perform qPCR with 2X Hy-SYBR power mix (using a different kit requires protocol adjustments) on a 96-well plate=== * Prepare a separate mix for each primer you are using. The SYBR mix is very viscous, so always prepare extra mix (~ x1.2) and mix well * Primers for total yeast quantification: YEASTF (5-GAGTCGAGTTGTTTGGGAATGC-3); YEASTR (5-TCTCTTTCCAAAGTTCTTTTCATCTT T-3)[https://www.dropbox.com/scl/fi/f0khsskz124gi5m7tvenu/Real-Time-Quantitative-PCR.pdf?rlkey=nd0jyn8..."
• 00:0600:06, 9 September 2024 diff hist +6‎ Preparing the plate ‎No edit summary current
• 00:0600:06, 9 September 2024 diff hist +285‎ Preparing the plate ‎No edit summary
• 00:0200:02, 9 September 2024 diff hist +6‎ Preparing the plate ‎No edit summary
• 00:0100:01, 9 September 2024 diff hist +27‎ Preparing the plate ‎No edit summary

8 September 2024

• 23:5823:58, 8 September 2024 diff hist −1‎ Preparing the plate ‎No edit summary
• 23:5823:58, 8 September 2024 diff hist 0‎ N File:PCR plate example.pdf ‎No edit summary current
• 23:4723:47, 8 September 2024 diff hist +173‎ N Preparing the plate ‎ Created page with "* First, plan your plate, then prepare your SYBR-primer mix, and dilute your DNA accordingly. * An example for a planned plate: Usually three technical triplicates are used."
• 23:4623:46, 8 September 2024 diff hist +112‎ Laboratory for insect nutrition and metabolomics ‎No edit summary current

1 April 2024

• 09:3609:36, 1 April 2024 diff hist 0‎ Analysing NGS ‎No edit summary current
• 09:3609:36, 1 April 2024 diff hist +3‎ Analysing NGS ‎No edit summary
• 09:3609:36, 1 April 2024 diff hist +197‎ Analysing NGS ‎No edit summary
• 09:3409:34, 1 April 2024 diff hist 0‎ Analysing NGS ‎No edit summary
• 09:3409:34, 1 April 2024 diff hist +917‎ Analysing NGS ‎No edit summary

26 March 2024

• 00:3400:34, 26 March 2024 diff hist +219‎ Orgenizing the data from the LCMS mechine ‎No edit summary current
• 00:3100:31, 26 March 2024 diff hist +191‎ N Orgenizing the data from the LCMS mechine ‎ Created page with "* In order to organize the data for further analysis , we use script in R * Please go over the presentations File:presentation1.ppt, File:presentation2.ppt, File:presentation2.ppt"
• 00:2800:28, 26 March 2024 diff hist +48‎ Laboratory for insect nutrition and metabolomics ‎No edit summary
• 00:1900:19, 26 March 2024 diff hist 0‎ N File:Standard curve.png ‎No edit summary current
• 00:1700:17, 26 March 2024 diff hist +465‎ Designing and testing the qPCR primers ‎No edit summary current
• 00:1400:14, 26 March 2024 diff hist +768‎ Designing and testing the qPCR primers ‎No edit summary
• 00:1100:11, 26 March 2024 diff hist +1‎ Designing and testing the qPCR primers ‎No edit summary
• 00:1000:10, 26 March 2024 diff hist 0‎ N File:Melting curve.png ‎No edit summary current
• 00:0900:09, 26 March 2024 diff hist +1‎ Designing and testing the qPCR primers ‎No edit summary Tag: Manual revert
• 00:0800:08, 26 March 2024 diff hist 0‎ N File:Melting curve2.png ‎No edit summary current
• 00:0700:07, 26 March 2024 diff hist −1‎ Designing and testing the qPCR primers ‎No edit summary
• 00:0600:06, 26 March 2024 diff hist +1‎ Designing and testing the qPCR primers ‎No edit summary
• 00:0500:05, 26 March 2024 diff hist 0‎ N File:Meting curve.png ‎No edit summary current
• 00:0300:03, 26 March 2024 diff hist −1‎ Designing and testing the qPCR primers ‎No edit summary

25 March 2024

• 23:2223:22, 25 March 2024 diff hist 0‎ Designing and testing the qPCR primers ‎No edit summary
• 23:2123:21, 25 March 2024 diff hist +29‎ Designing and testing the qPCR primers ‎No edit summary
• 23:1923:19, 25 March 2024 diff hist 0‎ N File:Melting curve.pdf ‎No edit summary current
• 23:1923:19, 25 March 2024 diff hist +20‎ Designing and testing the qPCR primers ‎No edit summary
• 23:1523:15, 25 March 2024 diff hist −6‎ Designing and testing the qPCR primers ‎No edit summary
• 23:1523:15, 25 March 2024 diff hist +1,059‎ Designing and testing the qPCR primers ‎No edit summary
• 23:1323:13, 25 March 2024 diff hist +872‎ Designing and testing the qPCR primers ‎No edit summary
• 11:0811:08, 25 March 2024 diff hist +80‎ Laboratory for insect nutrition and metabolomics ‎No edit summary
• 11:0611:06, 25 March 2024 diff hist +303‎ Designing and testing the qPCR primers ‎No edit summary