Laboratory for insect nutrition and metabolomics

Chemical analysis

• Fat content
• Protein Content
• Ash and Fiber content
• Fatty acids composition
• Amino acids composition

LCMS

• Sample preparation for LCMS
• Orgenizing the data from the LCMS mechine
• Analysis LCMS results

Molecular analysis

Extraction

• Extraction of DNA
• Extraction of RNA
• DNA extraction for genome sequencing

Purification

• How to interpret nano-drop results
• DNA samples purification
• RNA samples purification
• Checking the RNA integrity on gel electrophoresis

cDNA synthesis

• cDNA synthesis

RNA quantification (RT-PCR)

• Designing and testing the qPCR primers
• Experimental setting and analysing

DNA quantification (qPCR)

• Preparing the plate
• Preforming the PCR
• Prepering a standard curve to quantify the total yeast

submitting samples

• PacBio
• NGS

Sequencing

• analysing NGS
• assembling genomes
• Creating libraries for sequencing
• RNA-seq

Insect rearing

• Standard diet

Yeast rearing

• YPD broth