Maloof Lab:Protocols

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Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616


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DNA extraction for 96 well plates, CTAB method

DNA precipitation for 96 well plates, with glycogen

Making ultra-competent E.coli (Inoue method)

E. coli (DH5α chemically-competent) transformation

Agrobacterium transformation (Freeze-Thaw method)

Cleaning ball bearings

Arabidopsis seed sterilization

Miniprep using Promega reagents but not the columns

Recipes for Miniprep solutions

MS plates for plant growth

MS plates for plant growth (buffered, no sucrose)

Exo-SAP PCR cleanup for sequencing

Seed Storage

Eleven Golden Rules of qRT-PCR (Uvardi et al.)

Trizol extraction

Tomato seed TMV treatment and sterilization

N. Benthamian Agro Infiltration



Electrophoresis Buffers

Loading Dye and 1kb Ladder

PCR taq, buffer and MgCl2

Lab Safety

Harmer/Maloof labs' safety page

Lab Tasks

Maloof lab tasks

Undergrad Instructions

Scripts, instructions and miscellaneous

Using the Epson scanner

Using the LI1800 light meter

Using the Black Comet light meter

Sequence Analysis with eBioX

Analyzing luminiscence over time with ImageJ

Hypocotyl measurement with ImageJ

Maloof/Harmer Primer Database Search (Only internal users)

Patricia's Motif search (Only internal users)

Maloof Gene Expression Plot Tool in different tissues for M82 vs PEN (Only internal users)

Maloof Gene Expression Plot Tool for M82, PEN, HAB & PIM (Only internal users)

Laser Tough Tag Settings to print

How to print barcodes

PDA measurement system

Measuring time-lapse hypocotyl movies with ImageJ

Earth champion instructions