Maloof Lab:Seed Storage

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  • Let seed dry on plant until siliques are completely brown (but before they split and drop all of their seed)
  • Collect seed and place an aliquot in a 1.5ml screwcap eppondorf tube
  • Create a database entry for your seed in the seed database
  • Place eppendorf tube in desiccator with lid loosely placed on top
  • Keep in desiccator for two weeks after dessicator reaches 15% humidity
  • Remove from desiccator and tighten cap quickly
  • Store at -20°C
  • Let tube warm to room temperature before opening

Note: seeds can jump out of tubes if there is no lid on them

modified from NASC protocol