Maloof Lab:Seed Storage

• Let seed dry on plant until siliques are completely brown (but before they split and drop all of their seed)
• Collect seed and place an aliquot in a 1.5ml screwcap eppondorf tube
• Create a database entry for your seed in the seed database
• Place eppendorf tube in desiccator with lid loosely placed on top
• Keep in desiccator for two weeks after dessicator reaches 15% humidity
• Remove from desiccator and tighten cap quickly
• Store at -20°C
• Let tube warm to room temperature before opening

Note: seeds can jump out of tubes if there is no lid on them

modified from NASC protocol