Ethanol Seed Sterilization
- Tap seeds into 1.5mL tubes (labeled with VWR pen)
- Add 1mL 70% Ethanol, 0.1% Triton solution to each tube
- Vortex at 8 for 5 minutes
- Aspirate liquid off of seeds
- Add about 1mL 70% Ethanol to each tube
- In sterile hood, pipette seeds onto filter paper (labeled with pencil)
- Let Ethanol evaporate away from filter paper
- When filter paper is dry, tap seeds onto plates
- Tape plates, wrap in foil, and refrigerate at 4 degrees for 3-4 days before unwrapping and placing plates in growth chamber
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