Maloof Lab:antibiotics

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Antibiotic stocks

	abb	stock conc.	LB plate conc.	Vol. in 500ml LB agar	MS plate conc	Vol. in 1L MS
ampicilin	ap	50 mg/ml	100 μg/ml	1 ml
kanamycin	km/kn	50 mg/ml	50 μg/ml	0.5 ml	50 μg/ml	1 ml
spectinomycin	sp	100 mg/ml	100 μg/ml	0.5 ml
chloramphenicol	cm	34 mg/ml in ethanol	34 μg/ml	0.5 ml
rifampicin	rif	34 mg/ml in methanol	150 μg/ml	2.2 ml
tetracycline	tc/tet	12 mg/ml	12 μg/ml	0.5 ml
hygromycin	hyg	20 mg/ml			20 μg/ml	1 ml
carbenicillin	carb	100 mg/ml			500 μg/ml	5 ml
thiamine		50 mg/ml			50 μg/ml	1 ml
gentamycin*	gent	effective dose 6.5 mg/ml	15 μg/ml	0.75 ml	75 μg/ml	1 ml
basta		20 mg/ml			20 μg/ml	1 ml
zeocin	Zeo	100 mg/ml	25 μg/ml	0.125 ml
* the potency of gentamycin varies from stock to stock, the volume added must be calculated according to the potency of the stock