Maloof Lab:Exo SAP

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Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

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Exo-SAP PCR cleanup for sequencing


  1. Mix 10 uL PCR product with 4 uL Exo-SAP
  2. Incubate at 37° for 20 minutes (degrades primers and dephosphorylates dNTPs).
  3. Incubate at 80° for 15 minutes (destroys the enzymes).



Materials

  1. PCR product
  2. Exo-SAP (Amersham Biosciences, Cat#: US78200, or use the recipe below)
78 μL ddH2O
2 μL Exonuclease I (Amersham Biosciences, Cat# E70073X or E70073Z, 10U/uL)
20 μL Shrimp Alkaline Phosphatase (Amersham Biosciences, Cat# E70092X or E70092Z, 1U/uL)
100 μL Total