Maloof Lab:Exo SAP

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Exo-SAP PCR cleanup for sequencing Mix 10 uL PCR product with 4 uL Exo-SAP Incubate at 37° for 20 minutes (degrades primers and dephosphorylates dNTPs). Incubate at 80° for 15 minutes (destroys the enzymes). Materials PCR product Exo-SAP (Amersham Biosciences, Cat#: US78200, or use the recipe below) 78 μL ddH2O 2 μL Exonuclease I (Amersham Biosciences, Cat# E70073X or E70073Z, 10U/uL) 20 μL Shrimp Alkaline Phosphatase (Amersham Biosciences, Cat# E70092X or E70092Z, 1U/uL) 100 μL Total