Lidstrom:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 27: | Line 27: | ||
*[[Lidstrom:Ligation |Ligation (in progress)]] | *[[Lidstrom:Ligation |Ligation (in progress)]] | ||
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | *[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | ||
=====Fancy Manipulation===== | =====Fancy Manipulation===== | ||
Revision as of 07:11, 2 October 2012
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
DNA/RNA:
DNA
Manipulation
- Intro: Building with DNA (in progress)
- Digestion with Restriction Enzymes
- Gibson Assembly (In progress)
- Ligation (in progress)
- Overlap Extension PCR (in progress)
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Obervation
Misc.
- PCR (in progress)
- Loading Dye Recipe
- Purifying DNA (in progress)
- Miniprep (in progress)
- Oligo Orders (in progress)
- Diluting Primers
Protein
E. Coli
Enzyme Assays
General
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)
