IGEM:Harvard/2006/Cyanobacteria: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 68: | Line 68: | ||
*[[Media:Cyano_week4.ppt |Week 4 progress update]] | *[[Media:Cyano_week4.ppt |Week 4 progress update]] | ||
*[[Media:Cyanobacteria_Presentation_Week_5.ppt |Week 5 progress update, upd. 10:10 7/17]] | *[[Media:Cyanobacteria_Presentation_Week_5.ppt |Week 5 progress update, upd. 10:10 7/17]] | ||
*[[Media:Cyanobacteria_Presentation_Week_6.ppt |Week 6 progress update, upd. 10: | *[[Media:Cyanobacteria_Presentation_Week_6.ppt |Week 6 progress update, upd. 10:02 7/24 HH]] | ||
==Team Members== | ==Team Members== |
Revision as of 07:01, 24 July 2006
<html><style type='text/css'> .tabs {
font-size:80%; font-weight:none; width: 100%; color: #FFFFFF; background:#FFFFFF url("/images/5/54/DarkgreenTab-bg.gif") repeat-x bottom;
}
.tabs li {
background:url("/images/3/36/DarkgeenTab-left.gif") no-repeat left top;
}
.tabs a,.tabs strong {
background:url("/images/d/d3/DarkgreenTab-right.gif") no-repeat right top; color:#FFFFFF; padding: 3px 10px 3px 4px;
}
.tabs strong{
color:#CCFF00; background-image:url("/images/b/b1/DarkgreenTab-right_on.gif");
}
.tabs a:hover{
color:#66FF00;
}
</style></html>
Synthesis
Agenda
See image at right for our long-term project outline.
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/3/36/Cyanobacteria_Flowchart.png/300px-Cyanobacteria_Flowchart.png)
Monday 6/26
- Figure out optimal growing conditions (complete)
- Regrow cyanobacteria colonies (complete)
- Draft email questions for Professor Golden (complete)
Tuesday 6/27
- PCR crash course (complete)
- Picnic! (complete)
- Check first batch of colonies again (complete)
- Complete more research in preparation for Professor Golden (complete)
- Order primers (complete)
Wednesday 6/28
- Research transformation protocols (mostly complete)
- Start creating presentation for Friday
- Talk to Professor Golden (complete)
- Grow additional liquid cyanobacteria cultures (new order of PCC 7942 is not here yet)
- Finalize goals (work in cyanobacteria or in e. coli)/ plan out the rest of summer (in progress)
Thursday 6/29
- Research Sigma factors (on hold)
- Design and order our first set of primers (for entire KaiABC sequence) (complete)
- Make presentation for Friday (complete)
Friday 6/30
- Present before Fourth of July weekend (cancelled)
Wednesday 7/5
- Research plasmids to ask Prof. Golden (moved from Thursday)
- Also, don't forget to ask her about CO2 and aerating our cultures
- Find out what equipment we would need for measuring bioluminescence
Thursday 7/6
- Make a model of the cyanobacteria
- We missed ice cream at Maxwell-Dworkin
Friday 7/7
- Make a model of the cyanobacteria (complete)
- We should be receiving the shipment of PCC 7942, otherwise we will call ATCC for the tracking information
- PCR PCC7942 (complete-- turned out negative)
- Plate PCC7942 (complete)
- Grow PCC7942 in liquid culture, both with thiosulfate and without (complete)
- TGIF in Bauer Center (complete)
Monday 7/10
- Make frozen stocks of our new grown PCC 7942 (complete)
- Research PCR protocols for cyanobacteria (maybe?) (complete)
- PCR (complete)
- Research DNA synthesis companies (complete)
- Design sequence to be synthesized (incomplete)
- Research homologous recombination design (incomplete)
Presentations
- Project proposal (week 2)
- Week 3 progress update
- Built incubator and obtained WH8102, PCC7942, and PCC6803 strains
- Week 4 progress update
- Week 5 progress update, upd. 10:10 7/17
- Week 6 progress update, upd. 10:02 7/24 HH
Team Members
- Hetmann Hsieh (talk, edits)
- Jeffrey Lau (talk, edits)
- Zhipeng Sun (talk, edits)
- David Ramos (talk, edits)
Recent Changes
List of abbreviations:
- N
- This edit created a new page (also see list of new pages)
- m
- This is a minor edit
- b
- This edit was performed by a bot
- (±123)
- The page size changed by this number of bytes
14 June 2024
|
N 12:55 | UA Biophysics:Peptides and IA 3 changes history +1,837 [Elizabeth Suesca (3×)] | |||
|
12:55 (cur | prev) +44 Elizabeth Suesca talk contribs | ||||
|
12:54 (cur | prev) −68 Elizabeth Suesca talk contribs | ||||
N |
|
12:45 (cur | prev) +1,861 Elizabeth Suesca talk contribs (Created page with "Debido a la rápida aparición de mecanismos de resistencia a antibióticos en diferentes microorganismos patógenos potencialmente mortales a nivel mundial, existe un gran interés en la identificación de agentes antimicrobianos alternativos que puedan ayudar en el desarrollo de nuevas estrategias de tratamiento. Mediante la aplicación de una rutina de Deep Learning, desarrollada y publicada por el grupo de investigación proponente, se predecirá una lista de candida...") |
|
12:45 | Biophysics 3 changes history +204 [Elizabeth Suesca (3×)] | |||
|
12:45 (cur | prev) +4 Elizabeth Suesca talk contribs | ||||
|
12:44 (cur | prev) +1 Elizabeth Suesca talk contribs | ||||
|
12:29 (cur | prev) +199 Elizabeth Suesca talk contribs |
13 June 2024
14:39 | User creation log User account Yannvrb talk contribs was created by Yar talk contribs and password was sent by email |
|
13:57 | Magni:Back Door 2 changes history +199 [Lorenzo Pasotti (2×)] | |||
|
13:57 (cur | prev) +151 Lorenzo Pasotti talk contribs | ||||
|
13:54 (cur | prev) +48 Lorenzo Pasotti talk contribs (→E. coli strains) |
12 June 2024
|
N 13:10 | Karas Lab:YAEL Electrode Localization 2 changes history +859 [Aclesage (2×)] | |||
|
13:10 (cur | prev) +585 Aclesage talk contribs | ||||
N |
|
13:08 (cur | prev) +274 Aclesage talk contribs (Created page with "<b>Y</b>our <b>A</b>dvanced <b>E</b>lectrode <b>L</b>ocalizer (YAEL) is free and open-source software for localizing and visualizing electrodes. YAEL is a sister package to the RAVE software toolbox for the analysis of intracranial electroencephalogram (iEEG) data.") |
|
13:04 | Karas Lab:Lab Notebook 2 changes history +48 [Aclesage (2×)] | |||
|
13:04 (cur | prev) 0 Aclesage talk contribs | ||||
|
13:00 (cur | prev) +48 Aclesage talk contribs |
06:13 | Hu:Publications diffhist +33 Hugangqing talk contribs |
06:12 | Hu diffhist +44 Hugangqing talk contribs |
11 June 2024
N 19:16 | BioMicroCenter:PricingFY2025 diffhist +28,119 Stuart S. Levine talk contribs (Created page with "''' PRICING UPDATE 7/1/2024 ''' == SEQUENCING == Please note that samples submitted from '''CORE LAB'''s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason. <BR><BR> === NOVASEQ SEQUENCING === {| class="wikitable" border=1 ! NOVASEQ SEQUENCING !width=80| CORE LAB !width=80| MIT !width=80| Non-MIT !width=80| uni...") |
18:40 | BioMicroCenter:Pricing diffhist +54 Stuart S. Levine talk contribs (→COMPUTATIONAL SERVICES) |
|
08:18 | Forster Lab 2 changes history +153 [Anthony C. Forster (2×)] | |||
m |
|
08:18 (cur | prev) +4 Anthony C. Forster talk contribs (→The Boss!?) | |||
|
08:16 (cur | prev) +149 Anthony C. Forster talk contribs (→The Boss!?) |
08:03 | Pecreaux diffhist −1,628 Cedre-PecreauxLab talk contribs (→News!) |