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IGEM:Harvard/2006/Cyanobacteria: Difference between revisions
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IGEM:Harvard/2006/Cyanobacteria (view source)
Revision as of 12:25, 27 October 2006
, 27 October 2006→Outline of Findings
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**[[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-7-5]] | **[[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-7-5]] | ||
*07/10/06: Some computer modeling has been done to see the effect of multiple unsyncronized clocks on phosphorylation state output. | *07/10/06: Some computer modeling has been done to see the effect of multiple unsyncronized clocks on phosphorylation state output. | ||
**[[/IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-7- | **[[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-7-10]] | ||
*07/21/06: Upon having trouble with site-specific mutagenesis on the KaiA and KaiBC operons from the cyanobacterial genome, we have decided to pursue synthesis of the constructs in parallel with continued extraction attempts. | |||
**[[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-7-21]] | |||
*08/01/06: Preliminary success with site-specific mutagenesis. | |||
**[[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-8-1]] | |||
*10/25/06: Constructs for Stage I have been completed; ready to move to Stage I of Western Blotting, to verify expression of KaiC and interaction of KaiA and KaiB with KaiC. | *10/25/06: Constructs for Stage I have been completed; ready to move to Stage I of Western Blotting, to verify expression of KaiC and interaction of KaiA and KaiB with KaiC. | ||
**[[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-24]] | **[[IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-24]] | ||
