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Secondary antibody incubation

Incubated for 45 minutes, then transferred the liquid to tube labeled "IRDye 700" for reuse.

Final wash schedule

  • 2 10m washes of PBST
    • First wash started: 1050
    • Second wash started: 1216
  • 2 10m washes of PBS
    • First wash started: 1230
    • Second wash started: 1243 --note: we washed for only 5m due to time constraints
  • Rinse with distilled water when finished
  • Wrap in aluminum foil, store in 4C refrigerator until imaging

Anybody want some data?