User contributions for David Altman
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19 September 2023
- 10:0610:06, 19 September 2023 diff hist +1 Altman:Pictures No edit summary current
- 10:0610:06, 19 September 2023 diff hist +5 Altman:Pictures No edit summary
- 10:0510:05, 19 September 2023 diff hist 0 Altman:Pictures No edit summary
- 10:0510:05, 19 September 2023 diff hist 0 N File:SCRP symposium.jpg No edit summary current
- 10:0410:04, 19 September 2023 diff hist +124 Altman:Pictures No edit summary
- 10:0210:02, 19 September 2023 diff hist 0 N File:SCRP symposium.pdf No edit summary current
- 09:5809:58, 19 September 2023 diff hist +112 Altman →News current
8 September 2023
- 13:4313:43, 8 September 2023 diff hist −1 Altman:Lab Members No edit summary current
- 13:4313:43, 8 September 2023 diff hist +36 Altman:Lab Members No edit summary
- 13:3913:39, 8 September 2023 diff hist +8 Altman:Lab Members →Current Lab Members
- 13:3913:39, 8 September 2023 diff hist 0 N File:Margaris website.jpeg No edit summary current
5 September 2023
- 21:0921:09, 5 September 2023 diff hist −19 Altman:Lab Members →Collaborators
- 21:0721:07, 5 September 2023 diff hist +12 Altman:Lab Members →Collaborators
- 21:0721:07, 5 September 2023 diff hist 0 N File:Ciocanel website.jpeg No edit summary current
- 21:0621:06, 5 September 2023 diff hist +152 Altman:Lab Members →Collaborators
- 21:0121:01, 5 September 2023 diff hist +14 Altman:Lab Members →Collaborators
- 21:0121:01, 5 September 2023 diff hist −1 Altman:Lab Members →Collaborators
- 21:0121:01, 5 September 2023 diff hist 0 N File:Dawes website.jpeg No edit summary current
- 21:0021:00, 5 September 2023 diff hist +183 Altman:Lab Members →Collaborators
- 20:5120:51, 5 September 2023 diff hist +7 Altman:Lab Members →Current Lab Members
- 20:5020:50, 5 September 2023 diff hist 0 N File:Mathews website.jpeg No edit summary current
- 09:1509:15, 5 September 2023 diff hist 0 User:Altman →David Altman current
- 09:1409:14, 5 September 2023 diff hist 0 N File:ALTMAN CV 230905.pdf No edit summary current
- 09:1409:14, 5 September 2023 diff hist −2 User:Altman →David Altman
- 08:5808:58, 5 September 2023 diff hist +11 Altman:Lab Members →Current Lab Members
- 08:5808:58, 5 September 2023 diff hist 0 N File:Campi website.jpg No edit summary current
- 08:5808:58, 5 September 2023 diff hist 0 N File:Isaacson website.jpg No edit summary current
13 June 2023
- 22:0622:06, 13 June 2023 diff hist −3 Altman →Welcome to the Altman Lab!
- 22:0622:06, 13 June 2023 diff hist +57 Altman →Welcome to the Altman Lab!
- 22:0522:05, 13 June 2023 diff hist −3 Altman No edit summary
- 22:0522:05, 13 June 2023 diff hist 0 N File:Lablogo.jpg No edit summary current
- 21:2621:26, 13 June 2023 diff hist −1 Polymerizing and labeling actin No edit summary current
- 21:2621:26, 13 June 2023 diff hist +3 Polymerizing and labeling actin No edit summary
- 21:2521:25, 13 June 2023 diff hist +785 Polymerizing and labeling actin No edit summary
- 21:2021:20, 13 June 2023 diff hist +421 Polymerizing and labeling actin No edit summary
- 21:1521:15, 13 June 2023 diff hist +129 Polymerizing and labeling actin No edit summary
- 21:1421:14, 13 June 2023 diff hist +875 N Polymerizing and labeling actin Created page with "1) Thaw out 10 uL aliquot of the 2 mg/mL G-actin stock (Cytoskeleton, Inc). * MW actin: ~43 kDa 2) Add 30 uL of G-buffer to dilute sample to 0.5 mg/mL actin (a total volume of 40 uL) 3) Let sit on ice for at least 30 minutes 4) Add 3.5 uL of polymerization salts to the 40 uL sample * Final concentration of salts in the actin: 1 mM ATP, 50 mM KCl, 2 mM MgCl2 5) Add ~0.5 uL of 300 uM stock of TRITC-phalloidin (from the -20 freezer) Recipes G-buffer: 2 mM..."
- 21:1321:13, 13 June 2023 diff hist +879 N /Polymerizing and labeling actin Created page with "1) Thaw out 10 uL aliquot of the 2 mg/mL G-actin stock (Cytoskeleton, Inc). * (MW actin: ~43 kDa) 2) Add 30 uL of G-buffer to dilute sample to 0.5 mg/mL actin (a total volume of 40 uL) 3) Let sit on ice for at least 30 minutes 4) Add 3.5 uL of polymerization salts to the 40 uL sample * Final concentration of salts in the actin: 1 mM ATP, 50 mM KCl, 2 mM MgCl2 5) Add ~0.5 uL of 300 uM stock of TRITC-phalloidin (from the -20 freezer) Recipes G-buffer: 2 m..." current
- 21:1221:12, 13 June 2023 diff hist +80 Preparing Reagents No edit summary current
- 21:1021:10, 13 June 2023 diff hist +23 Gliding filament →Gliding Filament Assay current
- 21:1021:10, 13 June 2023 diff hist +23 Single Molecule Assays →Gliding Filament Assay current
- 21:0921:09, 13 June 2023 diff hist 0 Single Molecule Assays →Single Bead Optical Trap Assay using GFP-tagged motor
- 21:0921:09, 13 June 2023 diff hist +23 Single bead No edit summary current
- 21:0821:08, 13 June 2023 diff hist +23 Single Molecule Assays No edit summary
- 21:0721:07, 13 June 2023 diff hist +30 Studies of ARPE-19 Phagocytosis →Preparing POS fragments current
- 21:0521:05, 13 June 2023 diff hist +151 Preparing POS fragments No edit summary current
- 21:0421:04, 13 June 2023 diff hist +489 N Preparing POS fragments Created page with " 1) Re-suspend 10 POS in 5 mL of 10 mM HEPES * We have 1 M HEPES stock (pH 7.3) * Made 10 mM stock from 1 M frozen stock: 5 mL 1 M HEPES, raise to 500 mL and sterile filter 2) Pass 30 times through 20-gauge canula – in the cold room 3) Aliquot into 100 uL stocks 4) Assess protein concentration (Bradford assay) * From communciations with Linda Bapst, they measure ~1.4 ug/uL * We measured 450-650 ug/uL (standard curve was a little noisy) * Freeze in -80 for lon..."
- 21:0221:02, 13 June 2023 diff hist +500 N /Preparing POS fragments Created page with " 1) Re-suspend 10 POS in 5 mL of 10 mM HEPES * We have 1 M HEPES stock (pH 7.3) * Made 10 mM stock from 1 M frozen stock: 5 mL 1 M HEPES, raise to 500 mL and sterile filter 2) Pass 30 times through 20-gauge canula in the cold room 3) Aliquot into 100 uL stocks 4) Assess protein concentration (Bradford assay) * From communication with Dr. Linda Bapst, they measure 1.4 ug/uL * We measured 450-650 ug/uL (standard curve was a little noisy) * Freeze stocks of POS in..." current
- 20:5520:55, 13 June 2023 diff hist +66 Studies of ARPE-19 Phagocytosis →Studying phagocytosis in ARPE-19 cells
- 20:4920:49, 13 June 2023 diff hist 0 Coating beads with photoreceptor outer segments (POS) →Coating beads with photoreceptor outer segments (POS) current
