User contributions for Kristina M Holton

10 January 2008

• 20:4920:49, 10 January 2008 diff hist +74 N Quackenbush Lab:Research New page: == Breast Cancer == == Ovarian Cancer == == Colon Cancer == == CEGS == current
• 20:4020:40, 10 January 2008 diff hist −5 Template:Quackenbush No edit summary current
• 20:3720:37, 10 January 2008 diff hist +2,129 N Quackenbush Lab:Publications New page: == Selected Publications == * Aryee M, Quackenbush J [http://www.ncbi.nlm.nih.gov/sites/entrez?db=PubMed&cmd=search&term=17459168 An Optimized Predictive Strategy for Interactome Mappin... current

8 January 2008

• 20:0820:08, 8 January 2008 diff hist +33 Quackenbush Lab No edit summary current
• 20:0320:03, 8 January 2008 diff hist +1,057 Quackenbush Lab No edit summary
• 19:5119:51, 8 January 2008 diff hist +28 Template:Quackenbush No edit summary
• 19:4819:48, 8 January 2008 diff hist −344 Quackenbush Lab No edit summary
• 19:4719:47, 8 January 2008 diff hist +605 N Quackenbush Lab New page: <!--Possible lab page template. To use simply copy the following text into you lab page and then replace each instance of LabName with your own lab's name--> {{Template:Quackenbush}} <div...
• 19:4219:42, 8 January 2008 diff hist +601 N Quackenbush New page: <!--Possible lab page template. To use simply copy the following text into you lab page and then replace each instance of LabName with your own lab's name--> {{Template:Quackenbush}} <div... current
• 19:3919:39, 8 January 2008 diff hist 0 Template:Quackenbush No edit summary
• 19:3619:36, 8 January 2008 diff hist +61 N File:JQbanner.gif JQ - Functional Genomics and Computational Biology Laboratory current

4 January 2008

• 16:1616:16, 4 January 2008 diff hist +1,105 N Template:Quackenbush New page: 750px <div style="padding: 10px; color: #ffffff; background-color: #000; width: 730px"> <center> [[Quackenbush | <font face="trebuchet ms" style="color:#ffffff"> ''...

3 January 2008

• 19:4019:40, 3 January 2008 diff hist +2,668 N User:Kristina M Holton/Ovarian Notebook 2007 9 26 New page: ''Ambion RecoverAll Protocol: 12 samples'' Notes on Protocol: *Deparaffinization Step 2: Sample 3: Red; Sample 9: Brown *Deparaffinization Step 3: Sample 11: small pellet; Sample 12: smal... current
• 19:3819:38, 3 January 2008 diff hist +216 User:Kristina M Holton/Ovarian Notebook No edit summary current
• 19:3619:36, 3 January 2008 diff hist −36 User:Kristina M Holton/Ovarian Notebook No edit summary
• 19:3619:36, 3 January 2008 diff hist +2,751 N User:Kristina M Holton/Ovarian Notebook 2007 9 25 New page: ''Total RNA Extraction Protocol: SW480 & SW620 cell lines'' Day 2 ''BioAnalyzer: NanoChip on cell line samples'' ''Qiagen RNeasy FFPE kit: 12 samples Notes on Protocol: *Step 6: Samples... current
• 19:3519:35, 3 January 2008 diff hist −2,751 User:Kristina M Holton/Ovarian Notebook/2006-7-25 Removing all content from page current
• 19:3519:35, 3 January 2008 diff hist +123 N User:Kristina M Holton/Ovarian Notebook 2007 9 24 New page: ''Total RNA Extraction Protocol: SW480 & SW620 cell lines'' Use 9/17/07 protocol 1 plate each Overnight incubation at -20C current
• 19:3519:35, 3 January 2008 diff hist −123 User:Kristina M Holton/Ovarian Notebook/2006-7-24 Removing all content from page current
• 19:3419:34, 3 January 2008 diff hist +2,751 N User:Kristina M Holton/Ovarian Notebook/2006-7-25 New page: ''Total RNA Extraction Protocol: SW480 & SW620 cell lines'' Day 2 ''BioAnalyzer: NanoChip on cell line samples'' ''Qiagen RNeasy FFPE kit: 12 samples Notes on Protocol: *Step 6: Samples...
• 19:2719:27, 3 January 2008 diff hist +123 N User:Kristina M Holton/Ovarian Notebook/2006-7-24 New page: ''Total RNA Extraction Protocol: SW480 & SW620 cell lines'' Use 9/17/07 protocol 1 plate each Overnight incubation at -20C
• 19:2619:26, 3 January 2008 diff hist +971 N User:Kristina M Holton/Ovarian Notebook/2006-7-17 New page: ''BioAnalyzer: NanoChip on FFPE Samples'' ''BioAnalyzer: MicroChip on FFPE Samples'' ''Total & miRNA Extraction Protocol: SW480 & SW620 cell lines'' 1 plate each *Run 1: Used RW1 inste... current
• 19:2319:23, 3 January 2008 diff hist +1,285 N User:Kristina M Holton/Ovarian Notebook 2007 9 14 New page: ''Qiagen RNeasy FFPE Kit: G02, G03, Drapkin'' 10-10 micron samples, split into 2 tubes (50 microns each) *Step 5: Well-pelleted, no visible tumor sample in G03 *Step 7: White & cloudy *S... current
• 19:2119:21, 3 January 2008 diff hist 0 User:Kristina M Holton/Ovarian Notebook 2007 9 13 No edit summary current
• 19:2119:21, 3 January 2008 diff hist +4 User:Kristina M Holton/Ovarian Notebook 2007 9 13 No edit summary
• 19:2019:20, 3 January 2008 diff hist +1,261 N User:Kristina M Holton/Ovarian Notebook 2007 9 13 New page: Ambion Recoverall Kit: G02, G03, Drapkin 10-10 micron samples, split into 2 tubes (50 microns each) *Deparaffinization Step 2: still appreciable parafin **G03: No visible tumor sample aft...
• 19:0919:09, 3 January 2008 diff hist +123 User:Kristina M Holton/Ovarian Notebook No edit summary
• 19:0919:09, 3 January 2008 diff hist +8 User:Kristina M Holton/Ovarian Notebook No edit summary
• 19:0619:06, 3 January 2008 diff hist +265 User:Kristina M Holton/Ovarian Notebook 2007 7 17 No edit summary current
• 19:0519:05, 3 January 2008 diff hist +283 N User:Kristina M Holton/Ovarian Notebook 2007 7 17 New page: ''Bioanalyzer: 4 Ovarian Tissue Samples'' *Diluted 1:10 *Used Nano chip -> gel less viscous and easier to practice with *No rRNA bands, but miRNA bands ??? Suggestion: maybe too dilute *...
• 19:0319:03, 3 January 2008 diff hist −4 User:Kristina M Holton/Ovarian Notebook 2007 7 10 No edit summary current
• 19:0319:03, 3 January 2008 diff hist −4 User:Kristina M Holton/Ovarian Notebook 2007 7 9 No edit summary current
• 19:0219:02, 3 January 2008 diff hist −4 User:Kristina M Holton/Ovarian Notebook 2007 7 11 No edit summary current
• 19:0219:02, 3 January 2008 diff hist +448 N User:Kristina M Holton/Ovarian Notebook 2007 7 16 New page: ''Bioanalyzer for Ambion & Qiagen FFPE Kits'' First chip did not run -> voltage problem *Test plates OK *V=IR; V=Q/(eA/d) (V=Q/C) so...Q & I are fine, problem with loading gel (dialectric... current
• 19:0119:01, 3 January 2008 diff hist +479 N User:Kristina M Holton/Ovarian Notebook 2007 7 13 New page: ''Ambion RecoverAll Kit'' *Duodenum pellet - still small *Kidney pellet dropped in waste, then washed with EtOH and spun down again *Duodenum: slightly clear w/ precipitate; Kidney and Li... current
• 19:0019:00, 3 January 2008 diff hist +392 N User:Kristina M Holton/Ovarian Notebook 2007 7 11 New page: ''Qiagen FFPE Kit'' Three samples -> duodenum, kidney, liver *Duodenum->small pellet, not as turbid as kidney and liver when adding to gDNA column *Used the higher amount of the two *Proto...
• 18:5918:59, 3 January 2008 diff hist +58 N User:Kristina M Holton/Ovarian Notebook 2007 7 10 New page: '''Homogenizer still flaky-by 9,000 rpm display flashes'''
• 18:5818:58, 3 January 2008 diff hist +245 N User:Kristina M Holton/Ovarian Notebook 2007 7 9 New page: ''miRNA Extraction and Purification'' Patient: JBragger *Used 3ml QIAzol *First centrifuge step not needed *Split into 4 MiniPrep columns *Total yield: 240ul, 60ug '''When cleaning - hom...
• 18:5718:57, 3 January 2008 diff hist 0 User:Kristina M Holton/Ovarian Notebook 2007 7 3 No edit summary current
• 18:5618:56, 3 January 2008 diff hist 0 User:Kristina M Holton/Ovarian Notebook 2007 7 2 No edit summary current
• 18:5218:52, 3 January 2008 diff hist 0 User:Kristina M Holton/Ovarian Notebook 2007 7 6 No edit summary current
• 18:4918:49, 3 January 2008 diff hist +6 User:Kristina M Holton/Ovarian Notebook 2007 7 6 No edit summary
• 18:4918:49, 3 January 2008 diff hist +235 N User:Kristina M Holton/Ovarian Notebook 2007 7 6 New page: ''miRNA Extraction and Purification'' Patient: PB Used 3ml QIAzol First centrifugation step needed (step 9) Supernatant for tube 1->yellowish after 2nd centrifugation After MiniPrep tube 1...
• 18:4718:47, 3 January 2008 diff hist +12 User:Kristina M Holton/Ovarian Notebook 2007 7 2 No edit summary
• 18:4618:46, 3 January 2008 diff hist +3 User:Kristina M Holton/Ovarian Notebook 2007 7 3 No edit summary
• 18:4518:45, 3 January 2008 diff hist +203 N User:Kristina M Holton/Ovarian Notebook 2007 7 3 New page: ''miRNA Extraction and Purification Using 2nd Protocol'' Patient: DR 2ml might not be enough QIAzol for tissue grinder -> increased to about 3ml Did not need first centrifuge step Good yi...
• 18:4518:45, 3 January 2008 diff hist +859 N User:Kristina M Holton/Ovarian Notebook 2007 7 2 New page: ''miRNA Extraction using 1st protocol and Qiagen miRNEasy Kit'' Large Fibroma -> very very fatty Used 3ml TRIzol from Invitrogen While standing, TRIzol and fat start to pellet at the botto...
• 18:4418:44, 3 January 2008 diff hist +627 N User:Kristina M Holton/Ovarian Notebook New page: <calendar> name=User:Kristina_M_Holton/Ovarian_Notebook date=2006/07/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar> <calendar> name=User:Kristina_M_Holton/Ovar...
• 18:4118:41, 3 January 2008 diff hist −31 User:Kristina M Holton/Notebook No edit summary current
• 18:4018:40, 3 January 2008 diff hist +137 User:Kristina M Holton/Notebook No edit summary