User contributions for Kristina M Holton

10 January 2008

• 13:4913:49, 10 January 2008 diff hist +74‎ N Quackenbush Lab:Research ‎ New page: == Breast Cancer == == Ovarian Cancer == == Colon Cancer == == CEGS == current
• 13:4013:40, 10 January 2008 diff hist −5‎ Template:Quackenbush ‎No edit summary current
• 13:3713:37, 10 January 2008 diff hist +2,129‎ N Quackenbush Lab:Publications ‎ New page: == Selected Publications == * Aryee M, Quackenbush J [http://www.ncbi.nlm.nih.gov/sites/entrez?db=PubMed&cmd=search&term=17459168 An Optimized Predictive Strategy for Interactome Mappin... current

8 January 2008

• 13:0813:08, 8 January 2008 diff hist +33‎ Quackenbush Lab ‎No edit summary current
• 13:0313:03, 8 January 2008 diff hist +1,057‎ Quackenbush Lab ‎No edit summary
• 12:5112:51, 8 January 2008 diff hist +28‎ Template:Quackenbush ‎No edit summary
• 12:4812:48, 8 January 2008 diff hist −344‎ Quackenbush Lab ‎No edit summary
• 12:4712:47, 8 January 2008 diff hist +605‎ N Quackenbush Lab ‎ New page: <!--Possible lab page template. To use simply copy the following text into you lab page and then replace each instance of LabName with your own lab's name--> {{Template:Quackenbush}} <div...
• 12:4212:42, 8 January 2008 diff hist +601‎ N Quackenbush ‎ New page: <!--Possible lab page template. To use simply copy the following text into you lab page and then replace each instance of LabName with your own lab's name--> {{Template:Quackenbush}} <div... current
• 12:3912:39, 8 January 2008 diff hist 0‎ Template:Quackenbush ‎No edit summary
• 12:3612:36, 8 January 2008 diff hist +61‎ N File:JQbanner.gif ‎ JQ - Functional Genomics and Computational Biology Laboratory current

4 January 2008

• 09:1609:16, 4 January 2008 diff hist +1,105‎ N Template:Quackenbush ‎ New page: 750px <div style="padding: 10px; color: #ffffff; background-color: #000; width: 730px"> <center> [[Quackenbush | <font face="trebuchet ms" style="color:#ffffff"> ''...

3 January 2008

• 12:4012:40, 3 January 2008 diff hist +2,668‎ N User:Kristina M Holton/Ovarian Notebook 2007 9 26 ‎ New page: ''Ambion RecoverAll Protocol: 12 samples'' Notes on Protocol: *Deparaffinization Step 2: Sample 3: Red; Sample 9: Brown *Deparaffinization Step 3: Sample 11: small pellet; Sample 12: smal... current
• 12:3812:38, 3 January 2008 diff hist +216‎ User:Kristina M Holton/Ovarian Notebook ‎No edit summary current
• 12:3612:36, 3 January 2008 diff hist −36‎ User:Kristina M Holton/Ovarian Notebook ‎No edit summary
• 12:3612:36, 3 January 2008 diff hist +2,751‎ N User:Kristina M Holton/Ovarian Notebook 2007 9 25 ‎ New page: ''Total RNA Extraction Protocol: SW480 & SW620 cell lines'' Day 2 ''BioAnalyzer: NanoChip on cell line samples'' ''Qiagen RNeasy FFPE kit: 12 samples Notes on Protocol: *Step 6: Samples... current
• 12:3512:35, 3 January 2008 diff hist −2,751‎ User:Kristina M Holton/Ovarian Notebook/2006-7-25 ‎ Removing all content from page current
• 12:3512:35, 3 January 2008 diff hist +123‎ N User:Kristina M Holton/Ovarian Notebook 2007 9 24 ‎ New page: ''Total RNA Extraction Protocol: SW480 & SW620 cell lines'' Use 9/17/07 protocol 1 plate each Overnight incubation at -20C current
• 12:3512:35, 3 January 2008 diff hist −123‎ User:Kristina M Holton/Ovarian Notebook/2006-7-24 ‎ Removing all content from page current
• 12:3412:34, 3 January 2008 diff hist +2,751‎ N User:Kristina M Holton/Ovarian Notebook/2006-7-25 ‎ New page: ''Total RNA Extraction Protocol: SW480 & SW620 cell lines'' Day 2 ''BioAnalyzer: NanoChip on cell line samples'' ''Qiagen RNeasy FFPE kit: 12 samples Notes on Protocol: *Step 6: Samples...
• 12:2712:27, 3 January 2008 diff hist +123‎ N User:Kristina M Holton/Ovarian Notebook/2006-7-24 ‎ New page: ''Total RNA Extraction Protocol: SW480 & SW620 cell lines'' Use 9/17/07 protocol 1 plate each Overnight incubation at -20C
• 12:2612:26, 3 January 2008 diff hist +971‎ N User:Kristina M Holton/Ovarian Notebook/2006-7-17 ‎ New page: ''BioAnalyzer: NanoChip on FFPE Samples'' ''BioAnalyzer: MicroChip on FFPE Samples'' ''Total & miRNA Extraction Protocol: SW480 & SW620 cell lines'' 1 plate each *Run 1: Used RW1 inste... current
• 12:2312:23, 3 January 2008 diff hist +1,285‎ N User:Kristina M Holton/Ovarian Notebook 2007 9 14 ‎ New page: ''Qiagen RNeasy FFPE Kit: G02, G03, Drapkin'' 10-10 micron samples, split into 2 tubes (50 microns each) *Step 5: Well-pelleted, no visible tumor sample in G03 *Step 7: White & cloudy *S... current
• 12:2112:21, 3 January 2008 diff hist 0‎ User:Kristina M Holton/Ovarian Notebook 2007 9 13 ‎No edit summary current
• 12:2112:21, 3 January 2008 diff hist +4‎ User:Kristina M Holton/Ovarian Notebook 2007 9 13 ‎No edit summary
• 12:2012:20, 3 January 2008 diff hist +1,261‎ N User:Kristina M Holton/Ovarian Notebook 2007 9 13 ‎ New page: Ambion Recoverall Kit: G02, G03, Drapkin 10-10 micron samples, split into 2 tubes (50 microns each) *Deparaffinization Step 2: still appreciable parafin **G03: No visible tumor sample aft...
• 12:0912:09, 3 January 2008 diff hist +123‎ User:Kristina M Holton/Ovarian Notebook ‎No edit summary
• 12:0912:09, 3 January 2008 diff hist +8‎ User:Kristina M Holton/Ovarian Notebook ‎No edit summary
• 12:0612:06, 3 January 2008 diff hist +265‎ User:Kristina M Holton/Ovarian Notebook 2007 7 17 ‎No edit summary current
• 12:0512:05, 3 January 2008 diff hist +283‎ N User:Kristina M Holton/Ovarian Notebook 2007 7 17 ‎ New page: ''Bioanalyzer: 4 Ovarian Tissue Samples'' *Diluted 1:10 *Used Nano chip -> gel less viscous and easier to practice with *No rRNA bands, but miRNA bands ??? Suggestion: maybe too dilute *...
• 12:0312:03, 3 January 2008 diff hist −4‎ User:Kristina M Holton/Ovarian Notebook 2007 7 10 ‎No edit summary current
• 12:0312:03, 3 January 2008 diff hist −4‎ User:Kristina M Holton/Ovarian Notebook 2007 7 9 ‎No edit summary current
• 12:0212:02, 3 January 2008 diff hist −4‎ User:Kristina M Holton/Ovarian Notebook 2007 7 11 ‎No edit summary current
• 12:0212:02, 3 January 2008 diff hist +448‎ N User:Kristina M Holton/Ovarian Notebook 2007 7 16 ‎ New page: ''Bioanalyzer for Ambion & Qiagen FFPE Kits'' First chip did not run -> voltage problem *Test plates OK *V=IR; V=Q/(eA/d) (V=Q/C) so...Q & I are fine, problem with loading gel (dialectric... current
• 12:0112:01, 3 January 2008 diff hist +479‎ N User:Kristina M Holton/Ovarian Notebook 2007 7 13 ‎ New page: ''Ambion RecoverAll Kit'' *Duodenum pellet - still small *Kidney pellet dropped in waste, then washed with EtOH and spun down again *Duodenum: slightly clear w/ precipitate; Kidney and Li... current
• 12:0012:00, 3 January 2008 diff hist +392‎ N User:Kristina M Holton/Ovarian Notebook 2007 7 11 ‎ New page: ''Qiagen FFPE Kit'' Three samples -> duodenum, kidney, liver *Duodenum->small pellet, not as turbid as kidney and liver when adding to gDNA column *Used the higher amount of the two *Proto...
• 11:5911:59, 3 January 2008 diff hist +58‎ N User:Kristina M Holton/Ovarian Notebook 2007 7 10 ‎ New page: '''Homogenizer still flaky-by 9,000 rpm display flashes'''
• 11:5811:58, 3 January 2008 diff hist +245‎ N User:Kristina M Holton/Ovarian Notebook 2007 7 9 ‎ New page: ''miRNA Extraction and Purification'' Patient: JBragger *Used 3ml QIAzol *First centrifuge step not needed *Split into 4 MiniPrep columns *Total yield: 240ul, 60ug '''When cleaning - hom...
• 11:5711:57, 3 January 2008 diff hist 0‎ User:Kristina M Holton/Ovarian Notebook 2007 7 3 ‎No edit summary current
• 11:5611:56, 3 January 2008 diff hist 0‎ User:Kristina M Holton/Ovarian Notebook 2007 7 2 ‎No edit summary current
• 11:5211:52, 3 January 2008 diff hist 0‎ User:Kristina M Holton/Ovarian Notebook 2007 7 6 ‎No edit summary current
• 11:4911:49, 3 January 2008 diff hist +6‎ User:Kristina M Holton/Ovarian Notebook 2007 7 6 ‎No edit summary
• 11:4911:49, 3 January 2008 diff hist +235‎ N User:Kristina M Holton/Ovarian Notebook 2007 7 6 ‎ New page: ''miRNA Extraction and Purification'' Patient: PB Used 3ml QIAzol First centrifugation step needed (step 9) Supernatant for tube 1->yellowish after 2nd centrifugation After MiniPrep tube 1...
• 11:4711:47, 3 January 2008 diff hist +12‎ User:Kristina M Holton/Ovarian Notebook 2007 7 2 ‎No edit summary
• 11:4611:46, 3 January 2008 diff hist +3‎ User:Kristina M Holton/Ovarian Notebook 2007 7 3 ‎No edit summary
• 11:4511:45, 3 January 2008 diff hist +203‎ N User:Kristina M Holton/Ovarian Notebook 2007 7 3 ‎ New page: ''miRNA Extraction and Purification Using 2nd Protocol'' Patient: DR 2ml might not be enough QIAzol for tissue grinder -> increased to about 3ml Did not need first centrifuge step Good yi...
• 11:4511:45, 3 January 2008 diff hist +859‎ N User:Kristina M Holton/Ovarian Notebook 2007 7 2 ‎ New page: ''miRNA Extraction using 1st protocol and Qiagen miRNEasy Kit'' Large Fibroma -> very very fatty Used 3ml TRIzol from Invitrogen While standing, TRIzol and fat start to pellet at the botto...
• 11:4411:44, 3 January 2008 diff hist +627‎ N User:Kristina M Holton/Ovarian Notebook ‎ New page: <calendar> name=User:Kristina_M_Holton/Ovarian_Notebook date=2006/07/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar> <calendar> name=User:Kristina_M_Holton/Ovar...
• 11:4111:41, 3 January 2008 diff hist −31‎ User:Kristina M Holton/Notebook ‎No edit summary current
• 11:4011:40, 3 January 2008 diff hist +137‎ User:Kristina M Holton/Notebook ‎No edit summary