User:Kristina M Holton/Ovarian Notebook 2007 9 13

Ambion Recoverall Kit: G02, G03, Drapkin

10-10 micron samples, split into 2 tubes (50 microns each)

• Deparaffinization Step 2: still appreciable parafin
  • G03: No visible tumor sample after xylene spin-down
• Deparafiinization Step 4-1: Add EtOH, vortex = cloudy, poorly pelleted after 2 minute spin-down
• Deparaffinzation Step 4-2: Spin down 2.5 mins, slightly better

T=3hrs: samples did not fully clarify

Nucleic Acid Isolation Step 2: Only partial clarification after add Isolation Buffer, large amount tissue not digested

AR 9/13/07	ng/ul	260/280	260/230	Vol	Total	Dilution	Total to Illumina
G02	438.36	2.02	1.37	120ul	52ug	60ul + 202ul H2O	262ul
G03	99.45	2.06	.36	120ul	12ug	60ul	60ul
Drapkin	153.59	1.94	.77	120ul	18ug	60ul + 32ul H20	92ul